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Products:Signal Transduction >> Signaling Pathway >> Lipid Signaling >> Other
Overview
Product name
IGFBP1 protein (Active)
Protein description
Recombinant Human IGF-BP1, a 25.4 kDa protein consisting of 235 amino acid residues (Isoform A).
Expression host
E. coli
Properties
Purification notes
Greater than 98% by SDS-PAGE gel and HPLC analyses. Endotoxin level is less than 0.1 ng per µg (1EU/µg).
Biological activity
Biological Activity : The ED50, as determined by its ability to inhibit IGF-I induced proliferation of MCF-7, is < 0.5 µg/ml in the presence of 6 ng/ml of human IGF-I.
Form
Lyophilised:
Reconsitute to 1mg/ml with sterile water.
Storage instructions
The lyophilized protein is stable for a few weeks at room temperature, but best stored at -20°C.
Concentration
Concentration information loading...
Sequence notes
MAPWQCAPCS AEKLALCPPV SASCSEVTRS AGCGCCPMCA LPLGAACGVA TARCARGLSC RALPGEQQPL HALTRGQGAC VQESDASAPH AAEAGSPESP ESTEITEEEL LDNFHLMAPS EEDHSILWDA ISTYDGSKAL HVTNIKKWKE PCRIELYRVV ESLAKAQETS GEEISKFYLP NCNKNGFYHS RQCETSMDGE AGLCWCVYPW NGKRIPGSPE IRGDPNCQIY FNVQN
Research areas
Cardiovascular >> Atherosclerosis >> Diabetes associated
Signal Transduction >> Growth Factors/Hormones >> Insulin / Insulin-like
Signal Transduction >> Signaling Pathway >> Lipid Signaling >> Other
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Western blot - IGFBP1 protein (Active) (ab50216)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab50216 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent concentration. (Ab50216 can be used as a WB positive control in conjunction with ab94985.)
SDS-PAGE: Use at an assay dependent dilution.
FuncS: Use at an assay dependent dilution.
Protein info
Function
IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Promotes cell migration.
Sequence similarities
Contains 1 IGFBP N-terminal domain.
Contains 1 thyroglobulin type-1 domain.
Post-translational
modifications
Phosphorylated; probably by casein kinase II. Alters the affinity of the protein for IGFs.
Cellular localization
Secreted.
Target information above from: UniProt accessionP08833
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
IGFBP1 protein (Active) images:
Western blot - IGFBP1 protein (Active) (ab50216)
Anti-IGFBP1 antibody [33627.11] (ab94985) at 1 µg/ml + IGFBP1 protein (Active) (ab50216) at 0.1 µg
Secondary
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 30 seconds
References for IGFBP1 protein (Active) (ab50216)
ab50216 has not yet been referenced specifically in any publications.
Publishing research using ab50216? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"