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Products:Signal Transduction >> Growth Factors/Hormones >> Insulin / Insulin-like
Overview
Product name
Anti-IGFBP2 antibody
See all IGFBP2 products (7) ...
Description
Rabbit polyclonal to IGFBP2
Specificity
Recognizes bovine IGFBP 2 at 32kD. Slight cross reactivity with IGFBP 1, 3, 4 and 5.
Tested applications
IP, WBmore details
Cross reactivity
Reacts with
Mouse, Rabbit, Goat, Horse, Hamster, Cow, Human
Immunogen
Bovine IGFBP 2 purified by immunoaffinity and HPLC chromatographies from conditioned medium of Madin Darby Bovine Kidney cells.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
0.1M Tris HCl, pH 7.5, 50% glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> Other
Cancer >> Growth factors >> Insulin and insulin-like
Signal Transduction >> Growth Factors/Hormones >> Insulin / Insulin-like
Applications
Show applications key
Our Abpromise guarantee covers the use of ab4244 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP
Application notes
IP: 4µl immunoprecipitated IGFBP 2 from 600µl of concentrated T98G serum free culture supernatant.
WB: 1/1000 - 1/2000. This detects IGFBP 2 in concentrated T98G serum free culture supernatant. T98G cell culture supernatant was resolved by electrophoresis, transferred to nitrocellulose and probed with anti IGFBP 2 (1/1000). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Predicted molecular weight: 32kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Inhibits IGF-mediated growth and developmental rates. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
Sequence similarities
Contains 1 IGFBP N-terminal domain.
Contains 1 thyroglobulin type-1 domain.
Domain
The C-terminus is required for IGF-binding and growth inhibition.
Post-translational
modifications
O-glycosylated.
Cellular localization
Secreted.
Target information above from: UniProt accessionP18065
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BP 2 antibody
- BP2 antibody
- IBP 2 antibody
see all
Database links
- Entrez Gene: 282260 Cow
- Entrez Gene: 3485 Human
- Entrez Gene: 16008 Mouse
- Omim: 146731 Human
- SwissProt: P13384 Cow
- SwissProt: P18065 Human
- SwissProt: P47877 Mouse
- SwissProt: Q91VK7 Mouse
see all
References for Anti-IGFBP2 antibody (ab4244)
This product has been referenced in:
- Lawlor Ket al. Pathway-based biomarker search by high-throughput proteomics profiling of secretomes. J Proteome Res 8:1489-503 (2009).Read more (PubMed: 19199430) »
See 1 publication for this product
Publishing research using ab4244? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"