IGFBP2 antibody (ab4244)

Product Name 

IGFBP2 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to IGFBP2

Immunogen 

Bovine IGFBP 2 purified by immunoaffinity and HPLC chromatographies from conditioned medium of Madin Darby Bovine Kidney cells.

Reacts with 
(species key)

Hu, Ms, Cow, Goat, Hm, Hrs, Rb

Specificity 

Recognizes bovine IGFBP 2 at 32kD.
Slight cross reactivity with IGFBP 1, 3, 4 and 5.

Tested applications 
(see key)

IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
IP: 4µl immunoprecipitated IGFBP 2 from 600µl of concentrated T98G serum free culture supernatant.
WB: 1/1000 - 1/2000. This detects IGFBP 2 in concentrated T98G serum free culture supernatant. T98G cell culture supernatant was resolved by electrophoresis, transferred to nitrocellulose and probed with anti IGFBP 2 (1/1000). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Predicted molecular weight: 32kDa.
Not tested in other applications.

Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Secreted

Research areas 

Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> Other
Cancer >> Growth factors >> Insulin and insulin-like
Signal Transduction >> Growth Factors/Hormones >> Insulin / Insulin-like

Relevance 

IGFBP2 (insulin-like growth factor binding protein 2) is a member of the ISGBP family which bind various IGFs. IGFBP2 is overexpressed in a wide spectrum of other cancers, including glioma, prostate cancer, synovial sarcoma, neuroblastoma, colon cancer, adrenocortical cancer, lung cancer, Wilms' tumor, and hepatoblastoma. The overexpression of IGFBP2 also correlates with the aggressiveness of some tumors. IGFBP2 activates the expression of matrix metalloprotease 2, which contributes to cell invasiveness

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

0.1M Tris HCl, pH 7.5, 50% glycerol

Material safety datasheet (MSDS) for this product:
Glycerol MSDS

Form 

Liquid

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

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