Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Signal Transduction >> Growth Factors/Hormones >> Insulin / Insulin-like
Overview
Product name
Anti-IGFBP2 antibody
See all IGFBP2 products (7) ...
Description
Rabbit polyclonal to IGFBP2
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Sheep, Cow, Pig
Immunogen
Synthetic peptide within residues: GRKPLKSGMK ELAVFREKVT EQHRQMGKGG KHHLGLEEPK KLRPPPARTP , corresponding to internal sequence amino acids 180-229 of Human IGFBP2 (NP_000588).
GRKPLKSGMK ELAVFREKVT EQHRQMGKGG KHHLGLEEPK KLRPPPARTP
Positive control
HepG2 cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> Other
Cancer >> Growth factors >> Insulin and insulin-like
Signal Transduction >> Growth Factors/Hormones >> Insulin / Insulin-like
-
Western blot - IGFBP2 antibody (ab84964)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab84964 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 45 kDa (predicted molecular weight: 35 kDa).
Target
Function
Inhibits IGF-mediated growth and developmental rates. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
Sequence similarities
Contains 1 IGFBP N-terminal domain.
Contains 1 thyroglobulin type-1 domain.
Domain
The C-terminus is required for IGF-binding and growth inhibition.
Post-translational
modifications
O-glycosylated.
Cellular localization
Secreted.
Target information above from: UniProt accessionP18065
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BP 2 antibody
- BP2 antibody
- IBP 2 antibody
see all
Database links
- Entrez Gene: 282260 Cow
- Entrez Gene: 3485 Human
- Entrez Gene: 16008 Mouse
- Entrez Gene: 397064 Pig
- Entrez Gene: 25662 Rat
- Omim: 146731 Human
- SwissProt: P13384 Cow
- SwissProt: P18065 Human
see all
Anti-IGFBP2 antibody images:
Western blot - IGFBP2 antibody (ab84964)
Anti-IGFBP2 antibody (ab84964) at 1 µg/ml (5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg
Secondary
anti-Rabbit IgG HRP at 1/50000 dilution
Predicted band size : 35 kDa
Observed band size : 45 kDa (why is the actual band size different from the predicted?)
Gel concentration: 12%
References for Anti-IGFBP2 antibody (ab84964)
ab84964 has not yet been referenced specifically in any publications.
Publishing research using ab84964? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"