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Products:Signal Transduction >> Growth Factors/Hormones >> Insulin / Insulin-like
Overview
Product name
IGFBP2 protein (Active)
Protein description
Recombinant full length IGFBP2 (Human), a 31.5 kDa protein consisting of 289 amino acid residues including the IGF-BP domain and thyroglobulin type-I domain, expressed in Insect cells.
Properties
Purity
>95% by SDS-PAGE
Purification notes
Greater than 98% by SDS-PAGE gel and HPLC analyses. Endotoxin level is less than 0.1 ng per µg (1EU/µg).
Biological activity
Biological Activity : Determined by its ability to inhibit IGF-I induced proliferation of FDC-P1 cells.
Form
Lyophilised
Storage instructions
Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
Concentration
Concentration information loading...
Sequence notes
EVLFRCPPCT PERLAACGPP PVAPPAAVAA VAGGARMPCA ELVREPGCGC CSVCARLEGE ACQVYTPRCG QGLRCYPHPG SELPLQALVM GEGTCEKRRD AEYGASPEQV ADNGDDHSEG GLVENHVDST MNMLGGGGSA GRKPLKSGMK ELAVFREKVT EQHRQMGKGG KHHLGLEEPK KLRPPPARTP CQQELDQVLE RISTMRLPDE RGPLEHLYSL HIPNCDKHGL YNLKQCKMSL NGQRGECWCV NPNTGKLIQG APTIRGDPEC HLFYNEQQEA RGVHTQRMQ
Research areas
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> Other
Cancer >> Growth factors >> Insulin and insulin-like
Signal Transduction >> Growth Factors/Hormones >> Insulin / Insulin-like
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Western blot - IGFBP2 protein (Active) (ab63223)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab63223 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent concentration. (Ab63223 can be used as a WB positive control in conjunction with ab91404.)
FuncS: Use at an assay dependent dilution.
SDS-PAGE: Use at an assay dependent dilution.
Protein info
Function
Inhibits IGF-mediated growth and developmental rates. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
Sequence similarities
Contains 1 IGFBP N-terminal domain.
Contains 1 thyroglobulin type-1 domain.
Domain
The C-terminus is required for IGF-binding and growth inhibition.
Post-translational
modifications
O-glycosylated.
Cellular localization
Secreted.
Target information above from: UniProt accessionP18065
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
IGFBP2 protein (Active) images:
Western blot - IGFBP2 protein (Active) (ab63223)
Anti-IGFBP2 antibody (ab91404) at 1/500 dilution + IGFBP2 protein (Active) (ab63223) at 0.01 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 30 seconds
References for IGFBP2 protein (Active) (ab63223)
ab63223 has not yet been referenced specifically in any publications.
Publishing research using ab63223? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"