IGFBP3 antibody (ab4248)

Product Name 

IGFBP3 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to IGFBP3

Immunogen 

Recombinant full length protein (Human IGFBP 3 expressed in CHO cells).

Reacts with 

Hu

Does not react with 
(species key)

Rat, Cow, Hrs, Pig

Specificity 

Recognizes human IGFBP 3.
Slight crossreactivity with other IGF binding proteins: IGFBP 1: ~10%, IGFBP 2: <0.2%, IGFBP 4: <0.2%, IGFBP 5: <0.2%.

Tested applications 
(see key)

IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
IP: 4?l immunoprecipitated IGFBP 2 from 600?l of concentrated T98G serum free culture supernatant.
WB: 1/250 - 1/1000. Predicted molecular weight: 40 kDa.
Not tested in other applications.

Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Secreted

Research areas 

Cardiovascular >> Atherosclerosis >> Apoptosis
Cardiovascular >> Atherosclerosis >> Diabetes associated
Signal Transduction >> Growth Factors/Hormones >> Insulin / Insulin-like
Cardiovascular >> Angiogenesis >> Angiogenic Factors
Signal Transduction >> Protein Phosphorylation >> Tyrosine Phosphatases
Cell Biology >> Apoptosis >> Receptors >> Associated Proteins

Relevance 

Insulin like growth factor binding protein 3 (IGFBP3) is a member of the superfamily of insulin like growth factor (IGF) binding proteins which include six high affinity IGF binding proteins (IGFBP) and at least four low affinity binding proteins referred to as IGFBP related proteins (IGFBPrP). The IGFBP members are cysteine rich proteins with conserved cysteine residues clustered in the amino terminal and the carboxy terminal regions of the molecule. The cDNA sequence encoding the mature human IGFBP3 is fused to the signal peptide of CD33.
Human IGFBP3 is the major IGF binding protein in plasma where it exists in a ternary complex with IGFI or IGFII and an acid labile subunit. IGFBPs hold a central position in IGF ligand-receptor interactions through influences on both the bioavailability and distribution of IGFs in the extracellular environment. Insulin like growth factor binding protein 3 (IGFBP3) can modulate the mitogenic and metabolic effects of the insulin like growth factors (IGFs). Insulin like growth factor binding protein 3 (IGFBP3) is expressed in multiple tissues. The highest expression level is found in the non paranchymal cells of the liver. The expression levels are higher during extrauterine life and peak during puberty. Insulin like growth factors (IGFs) and IGF binding proteins (IGFBPs) play important roles in cell growth and differentiation. IGFBP3 is one of the factors in serum that is responsible for high serum induced apoptosis in PC3 cells, a prostate cancer cell line. IGFBP3 is important in controlling glucose homeostasis with increased urinary levels in type I diabetes with persistent microalbuminuria.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Whole antiserum

Storage buffer 

n/a

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

References for IGFBP3 antibody (ab4248)

  This product has been used in:

Walker G et al. Insulin-like growth factor binding proteins IGFBP3, IGFBP4, and IGFBP5 predict endocrine responsiveness in patients with ovarian cancer. Clin Cancer Res 13:1438-44 (2007). PubMed: 17332286

If you publish research using ab4248 please let us know so that we can cite the reference on this datasheet.

Customer reviews (feedback) regarding IGFBP3 antibody

Customer FAQs regarding IGFBP3 antibody

Protocols for IGFBP3 antibody

Price and availability of products related to IGFBP3 antibody

IGFBP3 antibody - related products: