Overview

  • Product nameIGFBP4 Human ELISA Kit
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 0.25 ng/ml
  • Range
    0.41 ng/ml - 100 ng/ml
  • Recovery

    95 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 97.38 86% - 106%
    Serum 93.98 83% - 105%
    Plasma 94.69 94% - 104%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s IGFBP4 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IGFBP4 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Human IGFBP4 coated on a 96-well plate. Standards and samples are pipetted into the wells and IGFBP4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IGFBP4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IGFBP4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionIGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
  • Sequence similaritiesContains 1 IGFBP N-terminal domain.
    Contains 1 thyroglobulin type-1 domain.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • AI875747
    • BP 4
    • BP4
    • Deb2
    • HT29 IGFBP
    • IBP 4
    • IBP-4
    • IBP4
    • IBP4_HUMAN
    • IGF binding protein 4
    • IGF BP4
    • IGF-binding protein 4
    • IGFBP 4
    • IGFBP-4
    • IGFBP4
    • Insulin like growth factor binding protein 4
    • Insulin-like growth factor-binding protein 4
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100542 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

IGFBP4 Human ELISA Kit images

  • Representative standard curve using ab100542

  • Representative standard curve using ab100542

Protocols

References for IGFBP4 Human ELISA Kit (ab100542)

ab100542 has not yet been referenced specifically in any publications.

Product Wall

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I would suggest reading through carefully Section 2 (Assay Summary) and Section 6 (Preparation of the Reagents).

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There is a Protocol booklet which can be found under the Protocol section (at the bottom of the datasheet - blue hyperlink) OR under the Protocol Tab (top right side) which can be downloaded. Could you please advis...

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Thank you for contacting us.

We have in our catalogue the IGFBP4 protein (active form). The reference is ab125753 and the link to the datasheet is http://www.abcam.com/ab125753.

A tissue that is recommended as a positive control for ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"