IGFBP4 Human ELISA Kit (ab100542)
- Product nameIGFBP4 Human ELISA Kit
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity< 0.25 ng/ml
- Range0.41 ng/ml - 100 ng/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 97.38 86% - 106% Serum 93.98 83% - 105% Plasma 94.69 94% - 104%
- Species reactivityReacts with: Human
- Product overview
ab100542 Human IGFBP4 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IGFBP4 in serum, plasma, cell culture supernatants and urine. This assay employs an antibody specific for Human IGFBP4 coated on a 96-well plate. Standards and samples are pipetted into the wells and IGFBP4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IGFBP4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IGFBP4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples
- Tested applicationsELISA more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 1 x 96 tests 20X Wash Buffer Concentrate Item B 1 x 25ml 600X HRP-Streptavidin Concentrate Item G 1 x 200µl Assay Diluent A diluent buffer, For Standard/Sample (serum/plasma) diluent. 1 x 30ml Assay Diluent B 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent. 1 x 15ml Detection Antibody IGF-BP-4.: biotinylated anti-human IGF-BP-4. 2 vials IGF BP4 Microplate (12 strips x 8 wells) coated with anti-human IGFBP-4 1 unit IGF BP4 Standard Human(recombinant) 2 vials Stop Solution Item I 1 x 8ml TMB One-Step Substrate Reagent Item H 1 x 12ml
Contains 1 thyroglobulin type-1 domain.
- AI875747BP 4BP4
- Deb2HT29 IGFBPIBP 4IBP-4IBP4IBP4_HUMANIGF binding protein 4IGF BP4IGF-binding protein 4IGFBP 4IGFBP-4IGFBP4Insulin like growth factor binding protein 4Insulin-like growth factor-binding protein 4
Our Abpromise guarantee covers the use of ab100542 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for IGFBP4 Human ELISA Kit (ab100542)
ab100542 has not yet been referenced specifically in any publications.