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Do you have an ELISA protocol for this antibody? |
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ANSWER: |
Thank you for your enquiry. This antibody was tested in Indirect ELISA using the immunogen human recombinant IGF-binding Protein 5. The immunogen was coated on microtiter plates, blocked and stained with ab4257 at a dilution of 1:50. We do not have a specific ELISA protocol for the antibody. I hope this information helps, please do not hesitate to contact us if you need any more advice or information. |
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Customer would like to know what band size is expected with ab4257. |
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ANSWER: |
Thank you very much for your patience, I have only just heard back from the antibody's originator. The molecular weight is 23 kDa (252 amino acids) according to a reference which describes all 8 ILGFBP isoforms. J. Kostecka, J. Blahovec. Endocrine Regulations. vol 33:90-94, 1999. Insulin-Like Growth Factor Binding Proteins and their Functions (Minireview) |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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