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Product Name
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IGFBP5 antibody
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See all IGFBP5 antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to IGFBP5
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Immunogen
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HPLC purified IGFBP 5 isolated from conditioned medium of transfected CHO cells.
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Reacts with
(species key)
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Hu, Rat
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Specificity
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IGFBP 1 = < 0.5%, IGFBP 2 = < 0.1%, IGFBP 3 = < 0.1%, IGFBP 4 = < 0.5%.
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Tested applications
(see key)
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IHC-P, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-P: 1/200 (from PMID 17575226)
IP: 4µl immunoprecipitates IGFBP 5 from 500µl concentrated T98G serum free culture supernatant.
WB: 1/1000. Detects IGFBP 5 in concentrated serum free T98G culture supernatant as well as fragments of 23 and 18kDa. Predicted molecular weight: 31 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Secreted
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Research areas
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Signal Transduction >> Growth Factors/Hormones >> Insulin / Insulin-like
Signal Transduction >> Signaling Pathway >> Lipid Signaling >> Other
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Relevance
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IGFBP5 is one of a family of proteins that bind to insulin-like growth factors (IGFs). IGFBP5 is a multifunctional protein which acts not only as a traditional binding protein but also functions as a growth factor independent of IGFs to stimulate bone formation. Currently there are seven named IGF-BPs that form high affinity complexes with both IGFI and IGFII. IGFBP5 is the major IGF-binding protein present in bone tissue and helps potentiate the action of IGFI on smooth muscle cells, fibroblasts or osteoblasts. Data shows that IGFBP5 acts as a growth inhibitor and pro-apoptotic agent in breast cancer cells. IGFBP5 overexpressing mice show an increase in neonatal mortality, reduced female fertility, whole-body growth inhibition and retarded muscle development.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Whole antiserum
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Storage buffer
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n/a
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this IGFBP5 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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References for IGFBP5 antibody (ab4255)
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This product has been used in:
Smyth JF et al. Antiestrogen therapy is active in selected ovarian cancer cases: the use of letrozole in estrogen receptor-positive patients. Clin Cancer Res 13:3617-22 (2007). PubMed: 17575226
If you publish research using ab4255 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to IGFBP5
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IGFBP5 antibody - more information
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Customer reviews (feedback) regarding IGFBP5 antibody |
Customer FAQs regarding IGFBP5 antibody |
Protocols for IGFBP5 antibody |
Price and availability of products related to IGFBP5 antibody |
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Contact Abcam with a Technical Enquiry about IGFBP5 antibody |
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