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Overview

  • Product nameIGFBP5 protein
  • Protein descriptionRecombinant human IGFBP5 protein (amino acids 21 -272), a 28.6 kDa protein consisting of 253 amino acid residues.
  • Expression hostE. coli

    • Properties

  • Purity> 95 % by SDS-PAGE
  • Purification notesGreater than 98% by SDS-PAGE gel and HPLC analyses.
  • FormLyophilised:Reconstitute in water to a concentration of 0.1-1.0 mg/ml.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 10mM Sodium citrate, pH 4
    Endotoxin level is less than 0.1 ng per µg (1EU/µg).
  • Concentration information loading...
  • Sequence notesMLGSFVHCEP CDEKALSMCP PSPLGCELVK EPGCGCCMTC ALAEGQSCGV YTERCAQGLR CLPRQDEEKP LHALLHGRGV CLNEKSYREQ VKIERDSREH EEPTTSEMAE ETYSPKIFRP KHTRISELKA EAVKKDRRKK LTQSKFVGGA ENTAHPRIIS APEMRQESEQ GPCRRHMEAS LQELKASPRM VPRAVYLPNC DRKGFYKRKQ CKPSRGRKRG ICWCVDKYGM KLPGMEYVDG DFQCHTFDSS NVS
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab49835 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    WB WB: Use at an assay dependent concentration. Ab49835 can be used as a WB positive control in conjunction with ab84229.
    Inhibition Assay Inhib: Use a concentration of 0.3 µg/ml. The ED50 was determined by its ability to inhibit IGF-II induced proliferation of MCF-7 is < 0.3 µg/ml in the presence of 15 ng/ml of human IGF-II.
    Functional Studies FuncS: Use at an assay dependent dilution.
    SDS-PAGE SDS-PAGE: Use at an assay dependent dilution.

    Protein info

    • Alternative names
        IBP 5IBP-5IBP5
        IBP5_HUMANIGF binding protein 5IGF BP5IGF-binding protein 5IGFBP 5IGFBP-5IGFBP5Insulin like growth factor binding protein 5Insulin-like growth factor-binding protein 5
      see all
  • FunctionIGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
  • Tissue specificityOsteosarcoma, and at lower levels in liver, kidney and brain.
  • Sequence similaritiesContains 1 IGFBP N-terminal domain.
    Contains 1 thyroglobulin type-1 domain.
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession P24593 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    IGFBP5 protein images

    • Western blot - IGFBP5 protein (ab49835)
      Western blot - IGFBP5 protein (ab49835)
      Anti-IGFBP5 antibody (Biotin) (ab84229) at 0.1 µg/ml + IGFBP5 protein (ab49835) at 0.01 µg

      Secondary
      Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040) at 1/5000 dilution
      developed using the ECL technique

      Performed under reducing conditions.

      Exposure time : 30 seconds

    References for IGFBP5 protein (ab49835)

    This product has been referenced in:
    • Abrass CK & Hansen KM Insulin-like growth factor-binding protein-5-induced laminin gamma1 transcription requires filamin A. J Biol Chem 285:12925-34 (2010). Read more (PubMed: 20167606) »

    See 1 Publication for this product

    Publishing research using ab49835 ? Please let us know so that we can cite the reference in this datasheet

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"