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Products:Signal Transduction >> Signaling Pathway >> Lipid Signaling >> Other
Overview
Product name
IGFBP5 protein
Protein description
Recombinant human IGFBP5 protein (amino acids 21 -272), a 28.6 kDa protein consisting of 253 amino acid residues.
Expression host
E. coli
Properties
Purity
>95% by SDS-PAGE
Purification notes
Greater than 98% by SDS-PAGE gel and HPLC analyses.
Form
Lyophilised:
Reconstitute in water to a concentration of 0.1-1.0 mg/ml.
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 10mM Sodium citrate, pH 4
Endotoxin level is less than 0.1 ng per µg (1EU/µg).
Concentration
Concentration information loading...
Sequence notes
MLGSFVHCEP CDEKALSMCP PSPLGCELVK EPGCGCCMTC ALAEGQSCGV YTERCAQGLR CLPRQDEEKP LHALLHGRGV CLNEKSYREQ VKIERDSREH EEPTTSEMAE ETYSPKIFRP KHTRISELKA EAVKKDRRKK LTQSKFVGGA ENTAHPRIIS APEMRQESEQ GPCRRHMEAS LQELKASPRM VPRAVYLPNC DRKGFYKRKQ CKPSRGRKRG ICWCVDKYGM KLPGMEYVDG DFQCHTFDSS NVS
Research areas
Signal Transduction >> Growth Factors/Hormones >> Insulin / Insulin-like
Signal Transduction >> Signaling Pathway >> Lipid Signaling >> Other
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Western blot - IGFBP5 protein (ab49835)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab49835 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent concentration. (Ab49835 can be used as a WB positive control in conjunction with ab84229.)
Inhib: Use a concentration of 0.3 µg/ml(The ED50 was determined by its ability to inhibit IGF-II induced proliferation of MCF-7 is < 0.3 µg/ml in the presence of 15 ng/ml of human IGF-II. )
FuncS: Use at an assay dependent dilution.
SDS-PAGE: Use at an assay dependent dilution.
Protein info
Function
IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
Tissue specificity
Osteosarcoma, and at lower levels in liver, kidney and brain.
Sequence similarities
Contains 1 IGFBP N-terminal domain.
Contains 1 thyroglobulin type-1 domain.
Cellular localization
Secreted.
Target information above from: UniProt accessionP24593
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
IGFBP5 protein images:
Western blot - IGFBP5 protein (ab49835)
Anti-IGFBP5 antibody (Biotin) (ab84229) at 0.1 µg/ml + IGFBP5 protein (ab49835) at 0.01 µg
Secondary
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 30 seconds
References for IGFBP5 protein (ab49835)
This product has been referenced in:
- Abrass CK & Hansen KM Insulin-like growth factor-binding protein-5-induced laminin gamma1 transcription requires filamin A. J Biol Chem 285:12925-34 (2010).Read more (PubMed: 20167606) »
See 1 publication for this product
Publishing research using ab49835? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"