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Product Name
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IGFBP5 protein
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Product type
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Proteins and Peptides
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Peptide
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Recombinant human IGFBP5 protein (amino acids 21 -272), a 28.6 kDa protein consisting of 253 amino acid residues.
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Tested applications
(see key)
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FuncS, Inhib, SDS-PAGE
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Application notes
(see key)
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Recommended dilutions
FuncS: Use at an assay dependent dilution.
Inhib: Use at a concentration of 0.3 µg/ml.
The ED50 was determined by its ability to inhibit IGF-II induced proliferation of MCF-7 is < 0.3 µg/ml in the presence of 15 ng/ml of human IGF-II.
SDS-PAGE: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Research areas
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Signal Transduction >> Growth Factors/Hormones >> Insulin / Insulin-like
Signal Transduction >> Signaling Pathway >> Lipid Signaling >> Other
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Relevance
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IGFBP5 is one of a family of proteins that bind to insulin-like growth factors (IGFs). IGFBP5 is a multifunctional protein which acts not only as a traditional binding protein but also functions as a growth factor independent of IGFs to stimulate bone formation. Currently there are seven named IGF-BPs that form high affinity complexes with both IGFI and IGFII. IGFBP5 is the major IGF-binding protein present in bone tissue and helps potentiate the action of IGFI on smooth muscle cells, fibroblasts or osteoblasts. Data shows that IGFBP5 acts as a growth inhibitor and pro-apoptotic agent in breast cancer cells. IGFBP5 overexpressing mice show an increase in neonatal mortality, reduced female fertility, whole-body growth inhibition and retarded muscle development.
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Purity
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>95% by SDS-PAGE
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Storage buffer
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Preservative: None
Constituents: 10mM Sodium citrate, pH 4
Endotoxin level is less than 0.1 ng per µg (1EU/µg).
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Purification notes
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Greater than 98% by SDS-PAGE gel and HPLC analyses.
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Form
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Lyophilised
Reconstitute in water to a concentration of 0.1-1.0 mg/ml.
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Expression system
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E. coli
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Sequence
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MLGSFVHCEP CDEKALSMCP PSPLGCELVK EPGCGCCMTC ALAEGQSCGV YTERCAQGLR CLPRQDEEKP LHALLHGRGV CLNEKSYREQ VKIERDSREH EEPTTSEMAE ETYSPKIFRP KHTRISELKA EAVKKDRRKK LTQSKFVGGA ENTAHPRIIS APEMRQESEQ GPCRRHMEAS LQELKASPRM VPRAVYLPNC DRKGFYKRKQ CKPSRGRKRG ICWCVDKYGM KLPGMEYVDG DFQCHTFDSS NVS
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this IGFBP5 protein is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to IGFBP5
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IGFBP5 protein - more information
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