IGFBP5 protein (ab49835)
Constituents: 10mM Sodium citrate, pH 4
Endotoxin level is less than 0.1 ng per µg (1EU/µg).
Our Abpromise guarantee covers the use of ab49835 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||WB: Use at an assay dependent concentration. Ab49835 can be used as a WB positive control in conjunction with ab84229.|
|Inhibition Assay||Inhib: Use a concentration of 0.3 µg/ml. The ED50 was determined by its ability to inhibit IGF-II induced proliferation of MCF-7 is < 0.3 µg/ml in the presence of 15 ng/ml of human IGF-II.|
|Functional Studies||FuncS: Use at an assay dependent dilution.|
|SDS-PAGE||SDS-PAGE: Use at an assay dependent dilution.|
- IBP 5IBP-5IBP5
- IBP5_HUMANIGF binding protein 5IGF BP5IGF-binding protein 5IGFBP 5IGFBP-5IGFBP5Insulin like growth factor binding protein 5Insulin-like growth factor-binding protein 5
Contains 1 thyroglobulin type-1 domain.
IGFBP5 protein images
Anti-IGFBP5 antibody (Biotin) (ab84229) at 0.1 µg/ml +
IGFBP5 protein (ab49835) at 0.01 µg
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 30 seconds