Anti-IGSF8 antibody (ab74890)

Overview

• Product nameAnti-IGSF8 antibodySee all IGSF8 primary antibodies ...
• Description
  Rabbit polyclonal to IGSF8
• Tested applicationsWB more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Cow
• Immunogen

  Synthetic peptide conjugated to KLH derived from within residues 450 - 550 of Human IGSF8.

  Read Abcam's proprietary immunogen policy

  (Peptide available as ab74889.)

• Positive controlThis antibody gave a positive signal in Human Brain Tissue Lysate.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 1% BSA, PBS, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Cancer
  • Tumor immunology
  • Tumor-associated antigens

  • Microbiology
  • Interspecies Interaction
  • Host Virus Interaction

  • Immunology
  • Immune System Diseases
  • Antiviral Signaling

Applications

Target

• FunctionMay play a key role in diverse functions ascribed to CD81 and CD9 such as oocytes fertilization or hepatitis C virus function. May regulate proliferation and differentiation of keratinocytes. May be a negative regulator of cell motility: suppresses T-cell mobility coordinately with CD81, associates with CD82 to suppress prostate cancer cell migration, regulates epidermoid cell reaggregation and motility on laminin-5 with CD9 and CD81 as key linkers. May also play a role on integrin-dependent morphology and motility functions. May participate in the regulation of neurite outgrowth and maintenance of the neural network in the adult brain.
• Tissue specificityExpressed in brain, kidney, testis, liver and placenta with moderate expression in all other tissues. Detected on a majority of B-cells, T-cells, and natural killer cells but not on monocytes, polynuclear cells and platelets.
• Sequence similaritiesContains 4 Ig-like C2-type (immunoglobulin-like) domains.
• DomainThe Ig-like C2-type domains 3 and 4 are required for interaction with CD81.
  The short cytoplasmic domain is very basic, interacts with membrane PIPs, and mediates plasma membrane localization.
• Cellular localizationCell membrane.

Target information above from: UniProt accession Q969P0 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 93185 Human
  • Entrez Gene: 140559 Mouse
  • Omim: 606644 Human
  • SwissProt: Q969P0 Human
  • SwissProt: Q8R366 Mouse
  • Unigene: 332012 Human
  • Unigene: 271717 Mouse

• Alternative names
  CD316 antibodyCD316 antigen antibodyCD81 partner 3 antibody

  CD81P3 antibodyEWI-2 antibodyEWI2 antibodyGlu-Trp-Ile EWI motif-containing protein 2 antibodyigsf-8 antibodyIgSF8 antibodyIGSF8_HUMAN antibodyImmunoglobulin superfamily member 8 antibodyImmunoglobulin superfamily member 8 precursor antibodyKCT-4 antibodyKCT4 antibodyKeratinocytes-associated transmembrane protein 4 antibodyLIR-D1 antibodyPGRL antibodyProstaglandin regulatory-like protein antibody

  see all

Anti-IGSF8 antibody images

• 

  Western blot - IGSF8 antibody (ab74890)
  Anti-IGSF8 antibody (ab74890) at 1 µg/ml + Brain (Human) Tissue Lysate - adult normal tissue (ab29466) at 10 µg
  
  Secondary
  Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
  
  Performed under reducing conditions.
  
  Predicted band size : 65 kDa
  Observed band size : 70 kDa (why is the actual band size different from the predicted?)
  
  
  Exposure time : 20 minutes