Western blot - IKB alpha antibody [E130] (ab32518)

Anti-IKB alpha antibody [E130] (ab32518) at 1/10000 dilution + HeLa cell lysate

Predicted band size : 36 kDa
Observed band size : 35 kDa (why is the actual band size different from the predicted?)

Immunohistochemistry (Paraffin-embedded sections) - IKB alpha antibody [E130] (ab32518)

Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using ab32518 at 1/50 dilution.

Immunoprecipitation - IKB alpha antibody [E130] (ab32518)

ab32518 used to immunoprecipitate IKB alpha from human HeLa whole cell lysate. The antibody was further used to Western blot the protein.

Lane 1 IKB alpha IP

Lane 2 Control immunoprecipitate

Lane 3 Input (20%)

This image is courtesy of an anonymous Abreview

Immunoprecipitation - IKB alpha antibody [E130] (ab32518)

ab32518 used to immunoprecipitate IKB alpha from rat PC12 whole cell lysate. The antibody was further used to Western blot the protein.

Lane 1 IKB alpha IP

Lane 2 Control immunoprecipitate

Lane 3 Input (20%)

This image is courtesy of an anonymous Abreview

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - IKB alpha antibody [E130] (ab32518)

ab32518 at 1/100 staining mouse kidney tissue sections by IHC-P. The tissue was paraformaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed before the tissue was blocked and incubated with the antibody for 1 hour. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

This image is courtesy of an anonymous Abreview

Immunocytochemistry/ Immunofluorescence - IKB alpha antibody [E130] (ab32518)

ICC/IF image of ab32518 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab32518, 1/1000 dilution) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.