You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Related products

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »
 

Anti-IKB alpha antibody [E130] (ab32518)

CodeSizePriceAbpointsAvailability
    
Add to basket
 
  • -
  •   
  •   
  •   
  •  
  •   
  •   
Updating...

Overview

Product name

Anti-IKB alpha antibody [E130]
See all IKB alpha products (25) ...

Description

Rabbit monoclonal [E130] to IKB alpha

Specificity

This antibody detects both the phosphorylated and non-phosphorylated form of the serine 32 region of IKB alpha.

Tested applications

WB, IHC-P, ICC, IP, IHC-Pmore details

Cross reactivity

Reacts with

Mouse, Rat, Hamster, Human

Predicted to work with

Cow, Pig

Immunogen

A synthetic peptide corresponding to residues surrounding phosphoserine 32 of human IkappaB-alpha. This antibody recognizes both phosphorylated and non-phosphorylated forms.

Positive control

Hela cell lysate and human prostate carcinoma tissue.

General notes

Produced under U.S. Patent No. 5,675,063.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine. pH 7.4

Purity

Tissue culture supernatant

Clonality

Monoclonal

Clone number

E130

Isotype

IgG

Research areas

Immunology >> Innate Immunity >> TLR Signaling
Cancer >> Signal transduction >> Nuclear signaling >> NFkB pathway
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> NFkB pathway
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> NFkB Pathway

  • Western blot - IKB alpha antibody [E130] (ab32518)Western blot - IKB alpha antibody [E130] (ab32518) image (enlarge)

  • Immunohistochemistry (Paraffin-embedded sections) - IKB alpha antibody [E130] (ab32518)Immunohistochemistry (Paraffin-embedded sections) - IKB alpha antibody [E130] (ab32518) image (enlarge)

  • Immunoprecipitation - IKB alpha antibody [E130] (ab32518)Immunoprecipitation - IKB alpha antibody [E130] (ab32518) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab32518 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ICC: 1/100.
IHC-P: 1/50 - 1/100.
IP: 1/25.
WB: 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 36 kDa).

Is unsuitable for FACS.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to tranlocate to the nucleus and activate transcription.

Involvement in disease

Defects in NFKBIA are the cause of ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant (ADEDAID) [MIM:612132]. Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. ADEDAID is an ectodermal dysplasia associated with decreased production of pro-inflammatory cytokines and certain interferons, rendering patients susceptible to infection.

Sequence similarities

Belongs to the NF-kappa-B inhibitor family.
Contains 5 ANK repeats.

Post-translational
modifications

Phosphorylated; disables inhibition of NF-kappa-B DNA-binding activity. Phosphorylation at positions 32 and 36 is prerequisite to recognition by UBE2D3 leading to polyubiquitination and subsequent degradation.
Sumoylated; sumoylation requires the presence of the nuclear import signal.
Monoubiquitinated at Lys-21 and/or Lys-22 by UBE2D3. Ubiquitin chain elongation is then performed by CDC34 in cooperation with the SCF(FBXW11) E3 ligase complex, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. The resulting polyubiquitinatin leads to protein degredation. Also ubiquitinated by SCF(BTRC) following stimulus-dependent phosphorylation at Ser-32 and Ser-36.
Deubiquitinated by porcine reproductive and respiratory syndrome virus Nsp2 protein, which thereby interefers with NFKBIA degradation and impairs subsequenbt NF-kappa-B activation.

Cellular localization

Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export.

Target information above from: UniProt accessionP25963 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • I kappa B alpha antibody
  • I kappa B alpha antibody
  • I(Kappa)B(alpha) antibody
  • I(Kappa)B(alpha) antibody
  • I-kappa-B-alpha antibody
  • IkappaBalpha antibody
  • IkB-alpha antibody
  • IKBA antibody
  • IKBA antibody
  • IKBA_HUMAN antibody
  • IKBalpha antibody
  • MAD 3 antibody
  • MAD 3 antibody
  • MAD3 antibody
  • Major histocompatibility complex enhancer binding protein MAD3 antibody
  • Major histocompatibility complex enhancer binding protein MAD3 antibody
  • Major histocompatibility complex enhancer-binding protein MAD3 antibody
  • NF kappa B inhibitor alpha antibody
  • NF-kappa-B inhibitor alpha antibody
  • NFKBI antibody
  • NFKBI antibody
  • NFKBIA antibody
  • NFKBIA antibody
  • Nuclear factor of kappa light chain gene enhancer in B cells antibody
  • Nuclear factor of kappa light chain gene enhancer in B cells antibody
  • Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha antibody
  • Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha antibody
see all

Database links

see all

Anti-IKB alpha antibody [E130] images:

  Western blot - IKB alpha antibody [E130] (ab32518)

Western blot - IKB alpha antibody [E130] (ab32518)

Anti-IKB alpha antibody [E130] (ab32518) at 1/10000 dilution + HeLa cell lysate

Predicted band size : 36 kDa
Observed band size : 35 kDa (why is the actual band size different from the predicted?)

  Immunohistochemistry (Paraffin-embedded sections) - IKB alpha antibody [E130] (ab32518)

Immunohistochemistry (Paraffin-embedded sections) - IKB alpha antibody [E130] (ab32518)

Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using ab32518 at 1/50 dilution.

  Immunoprecipitation - IKB alpha antibody [E130] (ab32518)

Immunoprecipitation - IKB alpha antibody [E130] (ab32518)

ab32518 used to immunoprecipitate IKB alpha from human HeLa whole cell lysate. The antibody was further used to Western blot the protein.

Lane 1 IKB alpha IP

Lane 2 Control immunoprecipitate

Lane 3 Input (20%)

This image is courtesy of an anonymous Abreview

See Abreview

  Immunoprecipitation - IKB alpha antibody [E130] (ab32518)

Immunoprecipitation - IKB alpha antibody [E130] (ab32518)

ab32518 used to immunoprecipitate IKB alpha from rat PC12 whole cell lysate. The antibody was further used to Western blot the protein.

Lane 1 IKB alpha IP

Lane 2 Control immunoprecipitate

Lane 3 Input (20%)

This image is courtesy of an anonymous Abreview

See Abreview

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - IKB alpha antibody [E130] (ab32518)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - IKB alpha antibody [E130] (ab32518)

ab32518 at 1/100 staining mouse kidney tissue sections by IHC-P. The tissue was paraformaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed before the tissue was blocked and incubated with the antibody for 1 hour. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

This image is courtesy of an anonymous Abreview

See Abreview

  Immunocytochemistry/ Immunofluorescence - IKB alpha antibody [E130] (ab32518)

Immunocytochemistry/ Immunofluorescence - IKB alpha antibody [E130] (ab32518)

ICC/IF image of ab32518 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab32518, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-IKB alpha antibody [E130] (ab32518)

This product has been referenced in:

  • King AEet al. An additive interaction between the NFkappaB and estrogen receptor signalling pathways in human endometrial epithelial cells. Hum Reprod 25:510-8 (2010). IHC-P; Human.Read more (PubMed: 19955102) »
  • Li ZHet al. Hepatitis C virus core protein induces malignant transformation of biliary epithelial cells by activating nuclear factor-kappaB pathway. J Gastroenterol Hepatol 25:1315-20 (2010). WB, IP; Human.Read more (PubMed: 20594262) »

See all 10 publications for this product

Publishing research using ab32518? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"