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Western blot - IKB alpha antibody [E130] (ab32518)
![Western blot - IKB alpha antibody [E130] (ab32518)](/ps/datasheet/images/av32518wb.gif)
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Immunohistochemistry (Paraffin-embedded sections) - IKB alpha antibody [E130] (ab32518)
![Immunohistochemistry (Paraffin-embedded sections) - IKB alpha antibody [E130] (ab32518)](/ps/datasheet/images/ab32518ihc.jpg)
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Immunoprecipitation - IKB alpha antibody [E130] (ab32518)
![Immunoprecipitation - IKB alpha antibody [E130] (ab32518)](/ps/datasheet/images/ab32518_2.jpg)
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Product Name
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IKB alpha antibody [E130]
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See all IKB alpha antibodies (19)...
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Product type
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Primary antibodies
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Description
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Rabbit monoclonal [E130] to IKB alpha
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Immunogen
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A synthetic peptide corresponding to human IkappaB-alpha.
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Reacts with
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Hu, Ms, Rat, Hm
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Predicted to react
(species key)
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Cow, Pig
(Due to sequence homology)
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Tested applications
(see key)
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ICC, IHC-P, IHC-P, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC: 1/100.
IHC-P: 1/50 - 1/100.
IP: 1/25.
WB: 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 36 kDa).
Is unsuitable for FACS.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Hela cell lysate and human prostate carcinoma tissue.
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Cellular localization
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Cytoplasmic
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Research areas
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Immunology >> Innate Immunity >> TLR Signaling
Chromatin and Nuclear Signaling >> Nuclear Signaling Pathways >> NFkB pathway
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> NFkB Pathway
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Relevance
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Three major forms of IKB like molecules have been identified and each is characterised by multiple copies of ankyrin repeats. IKB alpha and IKB beta appear to be the major regulatory forms of IKB in most cells. These proteins interact with p65 or cRel containing forms of NFkB and block nuclear import by masking the nuclear localisation sequences of NFkB. The activation of NFkB involves the inducible phosphorylation and subsequent degradation of IKB. Immunoblotting easily detects the hyperphosphorylated forms of IKB alpha, but not phosphorylated IKB beta. Interestingly, IKB alpha and IKB beta mediate different NFkB responses. IkB alpha appears to control more transient activation of NFkB in response to an inducer, while IKB beta controls a persistent response. Bcl3 interacts with p50 and p52 containing forms of NFkB, but rather than being an inhibitor it appears to function to stimulate transcription. The degradation of IKB is confirmed by immunoblotting.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Monoclonal
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Clone number
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E130
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Isotype
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IgG
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Purity
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Tissue culture supernatant
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Storage buffer
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Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine. pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Notes
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Produced under U.S. Patent No. 5,675,063.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this IKB alpha antibody [E130] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for IKB alpha antibody [E130] images, references, products related to ab32518 and other tools.
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IKB alpha antibody [E130] images:
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 Immunohistochemistry (Paraffin-embedded sections) - IKB alpha antibody [E130] (ab32518)
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using ab32518 at 1/50 dilution.
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Immunoprecipitation - IKB alpha antibody [E130] (ab32518)
ab32518 used to immunoprecipitate IKB alpha from human HeLa whole cell lysate. The antibody was further used to Western blot the protein. Lane 1 IKB alpha IP Lane 2 Control immunoprecipitate Lane 3 Input (20%) This image is courtesy of an anonymous Abreview
See Abreview
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Immunoprecipitation - IKB alpha antibody [E130] (ab32518)
![Immunoprecipitation - IKB alpha antibody [E130] (ab32518)](/ps/datasheet/images/ab32518_4.jpg)
ab32518 used to immunoprecipitate IKB alpha from rat PC12 whole cell lysate. The antibody was further used to Western blot the protein. Lane 1 IKB alpha IP Lane 2 Control immunoprecipitate Lane 3 Input (20%) This image is courtesy of an anonymous Abreview
See Abreview
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - IKB alpha antibody [E130] (ab32518)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - IKB alpha antibody [E130] (ab32518)](/ps/datasheet/images/ab32518_5.jpg)
ab32518 at 1/100 staining mouse kidney tissue sections by IHC-P. The tissue was paraformaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed before the tissue was blocked and incubated with the antibody for 1 hour. An HRP conjugated goat anti-rabbit antibody was used as the secondary. This image is courtesy of an anonymous Abreview
See Abreview
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Immunocytochemistry/ Immunofluorescence - IKB alpha antibody [E130] (ab32518)
![Immunocytochemistry/ Immunofluorescence - IKB alpha antibody [E130] (ab32518)](\ps\datasheet\images\32\ab32518\IKB-alpha-Primary-antibodies-ab32518-5.jpg)
ICC/IF image of ab32518 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab32518, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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References for IKB alpha antibody [E130] (ab32518)
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This product has been used in: (two most recent references)
King AE et al. An additive interaction between the NFkappaB and estrogen receptor signalling pathways in human endometrial epithelial cells. Hum Reprod 25:510-8 (2010). IHC-P; Human. PubMed: 19955102
Bournazou I et al. Apoptotic human cells inhibit migration of granulocytes via release of lactoferrin. J Clin Invest 119:20-32 (2009). WB; Human. PubMed: 19033648
See all 9 publication references for this product.
If you publish research using ab32518 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to IKB alpha
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IKB alpha antibody [E130] - more information
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Customer reviews (feedback) regarding IKB alpha antibody [E130] |
Customer FAQs regarding IKB alpha antibody [E130] |
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IKB alpha antibody [E130] - related products:
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IKB alpha antibody [E130] - other tools:
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Contact Abcam with a Technical Enquiry about IKB alpha antibody [E130] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
![Western blot - IKB alpha antibody [E130] (ab32518)](/index.html?datasheet=32518#av32518wb.gif)
![Immunohistochemistry (Paraffin-embedded sections) - IKB alpha antibody [E130] (ab32518)](/index.html?datasheet=32518#ab32518ihc.jpg)
![Immunoprecipitation - IKB alpha antibody [E130] (ab32518)](/index.html?datasheet=32518#ab32518_2.jpg)
