Products:Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> NFkB Pathway
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ab7217 has been referenced in 4 publications.
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ab7217 staining IKB alpha in human stomach tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using 10mM citrate buffer pH 6.0. Samples were then blocked with 5% serum for 1 hour at 23ºC followed by incubation with the primary antibody, at a 1/200 dilution, for 1 hour at 23ºC. An undiluted HRP-conjugated goat anti-rabbit IgG polyclonal was used as secondary antibody.
Image courtesy of an anonymous Abreview.
Whole cell lysate from HeLa cells used at total protein input 200µg. Immunoprecipitation step performed using Protein A/G. Diluted at 2µg/mg lysate, 16 hours at 4°C in NETN buffer.ab7217 used as western blot antibody at a 1/1000 dilution to confirm successful Immunoprecipitation.
Image courtesy of an anonymous Abreview.
ab7217 staining IKB alpha in human stomach tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with acetone and then blocked with 5% serum for 1 hour at 23ºC followed by incubation with the primary antibody at a 1/200 dilution for 1 hour at 23ºC. An undiluted HRP-conjugated goat anti-rabbit IgG polyclonal was used as secondary antibody.
Image courtesy of an anonymous Abreview.
ab7217 staining IKB alpha in NIH3T3 mouse fibroblast cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in formaldehyde, permeabilised in 0.025% Triton X and then blocked using 5% serum for 1 hour at 23ºC. Samples were then incubated with primary antibody at 1/500 for 1 hour at 23ºC. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 568 (red) used at a 1/1000 dilution.
Image courtesy of an anonymous Abreview.
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