Products:Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> NFkB Pathway
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Have you tested this antibody yet in mouse cells? If not, what is the peptide sequence of the immunogen (i.e. is the sequence the same for human and mouse?) Please let me know. |
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ANSWER: |
Thank you for your enquiry. Our source for this antibody has informed me that ab12135 does cross-react with mouse and so I have added this information to the online datasheet. Please contact us again if you have any additional questions. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Predicted band size : 35.6 kDa
Anti-IKB alpha (phospho S32 + S36) antibody [39A1431] (ab12135) at 1/500 dilution + Lysate prepared from human HeLa cells at 10 µg
Secondary
HRP-conjugated sheep monoclonal to mouse IgG at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 35.6 kDa
Observed band size : 40 kDa (why is the actual band size different from the predicted?)
Exposure time : 5 minutes
This image is a courtesy of Anonymous Abreview
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