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Anti-IKB alpha (phospho S32 + S36) antibody [39A1431] (ab12135)

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Overview

Product name

Anti-IKB alpha (phospho S32 + S36) antibody [39A1431]
See all IKB alpha products (25) ...

Description

Mouse monoclonal [39A1431] to IKB alpha (phospho S32 + S36)

Specificity

Ab12135 recognises IKB alpha (phosphorylated).

Tested applications

WB, Flow Cytmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

Synthetic peptide containing phosphorylated serines at amino acid residues 32 and 36 of human phosphorylated IKB alpha.

Positive control

Jurkat cells treated for 30 min with 100 µg/ml ALLN (N-Acetyl-Leu-Leu-Norleucinal), followed by incubation with or without 1 nM TNF-a cell extract.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: PBS

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Monoclonal

Clone number

39A1431

Isotype

IgG1

Research areas

Immunology >> Innate Immunity >> TLR Signaling
Cancer >> Signal transduction >> Nuclear signaling >> NFkB pathway
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> NFkB pathway
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> NFkB Pathway

  • Western blot - Anti-IKB alpha (phospho S32 + S36) antibody [39A1431] (ab12135)Western blot - Anti-IKB alpha (phospho S32 + S36) antibody [39A1431] (ab12135) image (enlarge)

  • Western blot - IKB alpha (phospho S32 + S36) antibody [39A1431] (ab12135)Western blot - IKB alpha (phospho S32 + S36) antibody [39A1431] (ab12135) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab12135 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to tranlocate to the nucleus and activate transcription.

Involvement in disease

Defects in NFKBIA are the cause of ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant (ADEDAID) [MIM:612132]. Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. ADEDAID is an ectodermal dysplasia associated with decreased production of pro-inflammatory cytokines and certain interferons, rendering patients susceptible to infection.

Sequence similarities

Belongs to the NF-kappa-B inhibitor family.
Contains 5 ANK repeats.

Post-translational
modifications

Phosphorylated; disables inhibition of NF-kappa-B DNA-binding activity. Phosphorylation at positions 32 and 36 is prerequisite to recognition by UBE2D3 leading to polyubiquitination and subsequent degradation.
Sumoylated; sumoylation requires the presence of the nuclear import signal.
Monoubiquitinated at Lys-21 and/or Lys-22 by UBE2D3. Ubiquitin chain elongation is then performed by CDC34 in cooperation with the SCF(FBXW11) E3 ligase complex, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. The resulting polyubiquitinatin leads to protein degredation. Also ubiquitinated by SCF(BTRC) following stimulus-dependent phosphorylation at Ser-32 and Ser-36.
Deubiquitinated by porcine reproductive and respiratory syndrome virus Nsp2 protein, which thereby interefers with NFKBIA degradation and impairs subsequenbt NF-kappa-B activation.

Cellular localization

Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export.

Target information above from: UniProt accessionP25963 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • I kappa B alpha antibody
  • I(Kappa)B(alpha) antibody
  • I-kappa-B-alpha antibody
  • IkappaBalpha antibody
  • IkB-alpha antibody
  • IKBA antibody
  • IKBA_HUMAN antibody
  • IKBalpha antibody
  • MAD 3 antibody
  • MAD3 antibody
  • Major histocompatibility complex enhancer binding protein MAD3 antibody
  • Major histocompatibility complex enhancer-binding protein MAD3 antibody
  • NF kappa B inhibitor alpha antibody
  • NF-kappa-B inhibitor alpha antibody
  • NFKBI antibody
  • NFKBIA antibody
  • Nuclear factor of kappa light chain gene enhancer in B cells antibody
  • Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha antibody
see all

Database links

see all

Anti-IKB alpha (phospho S32 + S36) antibody [39A1431] images:

  Western blot - Anti-IKB alpha (phospho S32 + S36) antibody [39A1431] (ab12135)

Western blot - Anti-IKB alpha (phospho S32 + S36) antibody [39A1431] (ab12135)



Predicted band size : 35.6 kDa

  Western blot - IKB alpha (phospho S32 + S36) antibody [39A1431] (ab12135)

Western blot - IKB alpha (phospho S32 + S36) antibody [39A1431] (ab12135)

Anti-IKB alpha (phospho S32 + S36) antibody [39A1431] (ab12135) at 1/500 dilution + Lysate prepared from human HeLa cells at 10 µg

Secondary
HRP-conjugated sheep monoclonal to mouse IgG at 1/5000 dilution
developed using the ECL technique

Performed under reducing conditions.

Predicted band size : 35.6 kDa
Observed band size : 40 kDa (why is the actual band size different from the predicted?)


Exposure time : 5 minutes

This image is a courtesy of Anonymous Abreview

See Abreview

References for Anti-IKB alpha (phospho S32 + S36) antibody [39A1431] (ab12135)

This product has been referenced in:

  • Li ZHet al. Hepatitis C virus core protein induces malignant transformation of biliary epithelial cells by activating nuclear factor-kappaB pathway. J Gastroenterol Hepatol 25:1315-20 (2010). WB; Human.Read more (PubMed: 20594262) »
  • Maalouf SWet al. Inflammatory responses in epithelia: endotoxin-induced IL-6 secretion and iNOS/NO production are differentially regulated in mouse mammary epithelial cells. J Inflamm (Lond) 7:58 (2010). WB; Mouse.Read more (PubMed: 21118556) »

See all 4 publications for this product

Publishing research using ab12135? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"