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Anti-IKB alpha (phospho S32 + S36) antibody [39A1431]
See all IKB alpha products (25) ...
Mouse monoclonal [39A1431] to IKB alpha (phospho S32 + S36)
Ab12135 recognises IKB alpha (phosphorylated).
WB, Flow Cytmore details
Reacts with
Mouse, Rat, Human
Synthetic peptide containing phosphorylated serines at amino acid residues 32 and 36 of human phosphorylated IKB alpha.
Jurkat cells treated for 30 min with 100 µg/ml ALLN (N-Acetyl-Leu-Leu-Norleucinal), followed by incubation with or without 1 nM TNF-a cell extract.
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: PBS
Concentration information loading...
Immunogen affinity purified
Monoclonal
39A1431
IgG1
Our Abpromise guarantee covers the use of ab12135 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 2 µg/ml.Detects a band of approximately 40 kDa (predicted molecular weight: 35.6 kDa).
Flow Cyt: Use at an assay dependent concentration.
Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to tranlocate to the nucleus and activate transcription.
Defects in NFKBIA are the cause of ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant (ADEDAID) [MIM:612132]. Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. ADEDAID is an ectodermal dysplasia associated with decreased production of pro-inflammatory cytokines and certain interferons, rendering patients susceptible to infection.
Belongs to the NF-kappa-B inhibitor family.
Contains 5 ANK repeats.
Phosphorylated; disables inhibition of NF-kappa-B DNA-binding activity. Phosphorylation at positions 32 and 36 is prerequisite to recognition by UBE2D3 leading to polyubiquitination and subsequent degradation.
Sumoylated; sumoylation requires the presence of the nuclear import signal.
Monoubiquitinated at Lys-21 and/or Lys-22 by UBE2D3. Ubiquitin chain elongation is then performed by CDC34 in cooperation with the SCF(FBXW11) E3 ligase complex, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. The resulting polyubiquitinatin leads to protein degredation. Also ubiquitinated by SCF(BTRC) following stimulus-dependent phosphorylation at Ser-32 and Ser-36.
Deubiquitinated by porcine reproductive and respiratory syndrome virus Nsp2 protein, which thereby interefers with NFKBIA degradation and impairs subsequenbt NF-kappa-B activation.
Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export.
Target information above from: UniProt accessionP25963
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Western blot - Anti-IKB alpha (phospho S32 + S36) antibody [39A1431] (ab12135)
![Western blot - Anti-IKB alpha (phospho S32 + S36) antibody [39A1431] (ab12135)](/ps/datasheet/Images/12/ab12135/a5.jpg)
Predicted band size : 35.6 kDa
Western blot - IKB alpha (phospho S32 + S36) antibody [39A1431] (ab12135)
![Western blot - IKB alpha (phospho S32 + S36) antibody [39A1431] (ab12135)](/ps/datasheet/images/12/ab12135/IKB-alpha-Primary-antibodies-ab12135-3.jpg)
Anti-IKB alpha (phospho S32 + S36) antibody [39A1431] (ab12135) at 1/500 dilution + Lysate prepared from human HeLa cells at 10 µg
Secondary
HRP-conjugated sheep monoclonal to mouse IgG at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 35.6 kDa
Observed band size : 40 kDa (why is the actual band size different from the predicted?)
Exposure time : 5 minutes
This image is a courtesy of Anonymous Abreview
This product has been referenced in:
See all 4 publications for this product
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![Western blot - Anti-IKB alpha (phospho S32 + S36) antibody [39A1431] (ab12135)](/ps/datasheet/Images/12/ab12135/a5.jpg)
Predicted band size : 35.6 kDa
![Western blot - IKB alpha (phospho S32 + S36) antibody [39A1431] (ab12135)](/ps/datasheet/images/12/ab12135/IKB-alpha-Primary-antibodies-ab12135-3.jpg)
Anti-IKB alpha (phospho S32 + S36) antibody [39A1431] (ab12135) at 1/500 dilution + Lysate prepared from human HeLa cells at 10 µg
Secondary
HRP-conjugated sheep monoclonal to mouse IgG at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 35.6 kDa
Observed band size : 40 kDa (why is the actual band size different from the predicted?)
Exposure time : 5 minutes
This image is a courtesy of Anonymous Abreview
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