IKB alpha (phospho S32 + S36) antibody [39A1431] (ab12135)

Product Name 

IKB alpha (phospho S32 + S36) antibody [39A1431]

Product type 

Primary antibodies

Description 

Mouse monoclonal [39A1431] to IKB alpha (phospho S32 + S36)

Immunogen 

Synthetic peptide containing phosphorylated serines at amino acid residues 32 and 36 of human phosphorylated IKB alpha.

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

Ab12135 recognises IKB alpha (phosphorylated).

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
WB: Use at a concentration of 1 - 2 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 35.6 kDa).

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Jurkat cells treated for 30 min with 100 µg/ml ALLN (N-Acetyl-Leu-Leu-Norleucinal), followed by incubation with or without 1 nM TNF-a cell extract.

Cellular localization 

Cytoplasmic

Research areas 

Immunology >> Innate Immunity >> TLR Signaling
Chromatin and Nuclear Signaling >> Nuclear Signaling Pathways >> NFkB pathway
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> NFkB Pathway

Relevance 

Three major forms of IKB like molecules have been identified and each is characterised by multiple copies of ankyrin repeats. IKB alpha and IKB beta appear to be the major regulatory forms of IKB in most cells. These proteins interact with p65 or cRel containing forms of NFkB and block nuclear import by masking the nuclear localisation sequences of NFkB. The activation of NFkB involves the inducible phosphorylation and subsequent degradation of IKB. Immunoblotting easily detects the hyperphosphorylated forms of IKB alpha, but not phosphorylated IKB beta. Interestingly, IKB alpha and IKB beta mediate different NFkB responses. IkB alpha appears to control more transient activation of NFkB in response to an inducer, while IKB beta controls a persistent response. Bcl3 interacts with p50 and p52 containing forms of NFkB, but rather than being an inhibitor it appears to function to stimulate transcription. The degradation of IKB is confirmed by immunoblotting.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

39A1431

Isotype 

IgG1

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: PBS

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.500 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

See below for IKB alpha (phospho S32 + S36) antibody [39A1431] images, references, products related to ab12135 and other tools.

IKB alpha (phospho S32 + S36) antibody [39A1431] images:

References for IKB alpha (phospho S32 + S36) antibody [39A1431] (ab12135)

  This product has been used in:

Cai B et al. Anti-inflammatory adjuvant in resuscitation fluids improves survival in hemorrhage. Crit Care Med 37:860-8 (2009). WB; Rat. PubMed: 19237889

If you publish research using ab12135 please let us know so that we can cite the reference on this datasheet.

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