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Products:Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> NFkB Pathway
Overview
Product name
Anti-IKB alpha (phospho S32 + S36) antibody
See all IKB alpha products (25) ...
Description
Rabbit polyclonal to IKB alpha (phospho S32 + S36)
Specificity
This antibody is specific for IKB alpha, only when phosphorylated at Serine 32/36.
Tested applications
WB, ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Cow, Human
Immunogen
A synthesized phosphopeptide derived from human IKB alpha around the phosphorylation site of Serine 32/36 (H-D-SP-G-L-D-SP-M-K)
Positive control
IHC: Breast carcinoma tissue
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab47752 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Predicted molecular weight: 36 kDa.
ELISA
ELISA: 1/10000ELISA: 1/10000
IHC-P: 1/50 - 1/100.
Target
Function
Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to tranlocate to the nucleus and activate transcription.
Involvement in disease
Defects in NFKBIA are the cause of ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant (ADEDAID) [MIM:612132]. Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. ADEDAID is an ectodermal dysplasia associated with decreased production of pro-inflammatory cytokines and certain interferons, rendering patients susceptible to infection.
Sequence similarities
Belongs to the NF-kappa-B inhibitor family.
Contains 5 ANK repeats.
Post-translational
modifications
Phosphorylated; disables inhibition of NF-kappa-B DNA-binding activity. Phosphorylation at positions 32 and 36 is prerequisite to recognition by UBE2D3 leading to polyubiquitination and subsequent degradation.
Sumoylated; sumoylation requires the presence of the nuclear import signal.
Monoubiquitinated at Lys-21 and/or Lys-22 by UBE2D3. Ubiquitin chain elongation is then performed by CDC34 in cooperation with the SCF(FBXW11) E3 ligase complex, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. The resulting polyubiquitinatin leads to protein degredation. Also ubiquitinated by SCF(BTRC) following stimulus-dependent phosphorylation at Ser-32 and Ser-36.
Deubiquitinated by porcine reproductive and respiratory syndrome virus Nsp2 protein, which thereby interefers with NFKBIA degradation and impairs subsequenbt NF-kappa-B activation.
Cellular localization
Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export.
Target information above from: UniProt accessionP25963
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- I kappa B alpha antibody
- I-kappa-B-alpha antibody
- IkappaBalpha antibody
see all
Database links
- Entrez Gene: 282291 Cow
- Entrez Gene: 4792 Human
- Entrez Gene: 18035 Mouse
- Entrez Gene: 25493 Rat
- Omim: 164008 Human
- SwissProt: P25963 Human
- SwissProt: Q9Z1E3 Mouse
- SwissProt: Q63746 Rat
see all
Anti-IKB alpha (phospho S32 + S36) antibody images:
Immunohistochemistry (Paraffin-embedded sections) - IKB alpha (phospho S32 + S36) antibody (ab47752)
Immunohistochemical analysis of paraffin embedded breast carcinoma using ab47752 at a 1/50 dilution.
Left image: Untreated
Right image: Treated with synthesized phosphopeptide
Western blot - IKB alpha (phospho S32 + S36) antibody (ab47752)
Anti-IKB alpha (phospho S32 + S36) antibody (ab47752) at 1/500 dilution + Lysate was prepared from human Huh-7 cells at 15000 cells
Secondary
HRP-conjugated sheep polyclonal to rabbit IgG at 1/20000 dilution
Performed under reducing conditions.
Predicted band size : 36 kDa
Observed band size : 36 kDa
Exposure time : 3 minutes
This image is a courtesy of Anonymous Abreview
References for Anti-IKB alpha (phospho S32 + S36) antibody (ab47752)
ab47752 has not yet been referenced specifically in any publications.
Publishing research using ab47752? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"