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Overview

  • Product nameIKB beta protein (Tagged-His Tag)See all IKB beta proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 9-356 of Human IKB beta, with a N-terminal His tag, 50kDa, Uniprot: Q15653.
  • Expression hostE. coli

    • Properties

  • Purification notes"Purity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation."
  • FormLyophilised:reconstitution with 124 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Sequence notesKAADADEWCDSGLGSLGPDAAAPGGPGLGAELGPGLSWAP LVFGYVTEDGDTA LHLAVIHQHEPFLDFLLGFSAGTEY MDLQNDLGQTALHLAAILGETSTVEKLY AAGAGLCVAE RRGHTALHLACRVGAHACARALLQPRPRRPREAPDTYLAQ GPD RTPDTNHTPVALYPDSDLEKEEEESEEDWKLQLEA ENYEGHTPLHVAVIHKDV EMVRLLRDAGADLDKPEPTC GRSPLHLAVEAQAADVLELLLRAGANPAARMYG GRTPL GSAMLRPNPILARLLRAHGAPEPEGEDEKSGPCSSSSDSD SGDEGDEY DDIVVHSSRSQTRLPPTPASKPLPDDPRPV
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91705 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
  • Application notesSDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        I kappa B betaI-kappa-B-betaIkappaBbeta
        IKB betaIkB-BIkB-betaIKBBIKBB_HUMANIkBbetaNF kappa BIBNF-kappa-B inhibitor betaNF-kappa-BIBNFKBIBThyroid receptor interacting protein 9Thyroid receptor-interacting protein 9TR interacting protein 9TR-interacting protein 9TRIP-9TRIP9
      see all
  • FunctionInhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. However, the unphosphorylated form resynthesized after cell stimulation is able to bind NF-kappa-B allowing its transport to the nucleus and protecting it to further NFKBIA-dependent inactivation. Association with inhibitor kappa B-interacting NKIRAS1 and NKIRAS2 prevent its phosphorylation rendering it more resistant to degradation, explaining its slower degradation.
  • Tissue specificityExpressed in all tissues examined.
  • Sequence similaritiesBelongs to the NF-kappa-B inhibitor family.
    Contains 6 ANK repeats.
  • Post-translational
    modifications    Phosphorylated by RPS6KA1; followed by degradation. Interaction with NKIRAS1 and NKIRAS2 probably prevents phosphorylation.
  • Cellular localizationCytoplasm. Nucleus.

    • Target information above from: UniProt accession Q15653 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    IKB beta protein (Tagged-His Tag) images

    • SDS-PAGE - IKB beta protein (Tagged-His Tag) (ab91705)
      SDS-PAGE - IKB beta protein (Tagged-His Tag) (ab91705)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images colored control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for IKB beta protein (Tagged-His Tag) (ab91705)

    ab91705 has not yet been referenced specifically in any publications.

    Publishing research using ab91705 ? Please let us know so that we can cite the reference in this datasheet

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