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IKB epsilon protein (Tagged-His Tag) (ab91869)

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Overview

  • Product nameIKB epsilon protein (Tagged-His Tag)
  • Protein descriptionRecombinant fragment, corresponding to amino acids 319-500 of Human IKB epsilon with N terminal His tag; 182 amino acids, 22kDa (19.7kDa predicted from sequence). UniProt ID = O00221.
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 92 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Sequence notesSRALQDRHGDTALHVACQRQHLACARCLLEGRPEPGRGTS HSLDLQLQNWQGLACLHIAT LQKNQPLMELLLRNGADI DVQEGTSGKTALHLAVETQERGLVQFLLQAGAQVDARMLN GC TPLHLAAGRGLMGISSTLCKAGADSLLRNVEDETPQ DLTEESLVLLPFDDLKISGKLLLCTD
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91869 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
  • Application notesSDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        I kappa B epsilonI-kappa-B-epsilonIkappaBepsilon
        IkB EIkB-EIkB-epsilonIKBEIKBE_HUMANMGC72568NF kappa B inhibitor epsilonNF kappa BIENF-kappa-B inhibitor epsilonNF-kappa-BIENFkappa BIENFkappaB inhibitor epsilonNFKBIENuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor epsilonOTTHUMP00000016522Slc35b2solute carrier family 35, member B2
      see all
  • FunctionInhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. Inhibits DNA-binding of NF-kappa-B p50-p65 and p50-c-Rel complexes.
  • Tissue specificityHighly expressed in spleen, testis and lung, followed by kidney, pancreas, heart, placenta and brain. Also expressed in granulocytes and macrophages.
  • Sequence similaritiesBelongs to the NF-kappa-B inhibitor family.
    Contains 6 ANK repeats.
  • Post-translational
    modifications    Serine phosphorylated; followed by proteasome-dependent degradation.
  • Cellular localizationCytoplasm.

    • Target information above from: UniProt accession O00221 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    IKB epsilon protein (Tagged-His Tag) images

    • SDS-PAGE - IKB epsilon protein (Tagged-His Tag) (ab91869)
      SDS-PAGE - IKB epsilon protein (Tagged-His Tag) (ab91869)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for IKB epsilon protein (Tagged-His Tag) (ab91869)

    ab91869 has not yet been referenced specifically in any publications.

    Publishing research using ab91869 ? Please let us know so that we can cite the reference in this datasheet

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