Overview
- Product nameAnti-IKK alpha + IKK beta antibodySee all IKK alpha + IKK beta primary antibodies ...
- DescriptionRabbit polyclonal to IKK alpha + IKK beta
- Specificityab62481 detects endogenous levels of total IKK-a/ IKK-ß protein only when phosphorylated at serine 180/181.
- Tested applicationsIHC-P, ELISA more details
- Species reactivityReacts with: Human
Predicted to work with: Mouse - Immunogen
Synthetic non-phosphopeptide (Human) derived from around the phosphorylation site of serine 180/181 (C-T-SP-F-V).
Properties
- FormLiquid
- Storage instructionsStore at -20°C. Stable for 12 months at -20°C
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 -
Concentration information loading... - PurityImmunogen affinity purified
- Purification notesThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab62481 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| IHC-P | |
| ELISA |
IHC-P: 1/50 - 1/100.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
- RelevanceIKK is a serine protein kinase, and the IKK complex contains alpha and beta subunits (IKK alpha and IKK beta). IKK alpha and IKK beta interact with each other and both are essential for NFkB activation.
- Cellular localizationCytoplasmic and Nuclear
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Database links
- Entrez Gene: 1147 Human
- Entrez Gene: 3551 Human
- Entrez Gene: 12675 Mouse
- Entrez Gene: 16150 Mouse
- Omim: 600664 Human
- Omim: 603258 Human
- SwissProt: O15111 Human
- SwissProt: O14920 Human
- SwissProt: Q60680 Mouse
- SwissProt: O88351 Mouse
- Unigene: 198998 Human
- Unigene: 413513 Human
see all
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Alternative names
- CHUK antibodyConserved helix loop helix ubiquitous kinase antibodyI kappa B kinase 1 antibody
- I kappa B kinase 2 antibodyI Kappa B kinase alpha antibodyI Kappa B kinase beta antibodyIkappaB kinase antibodyIkBKA antibodyIkBKB antibodyIKK alpha antibodyIKK B antibodyIKK beta antibodyIKK1 antibodyIKK2 antibodyIKKA antibodyIKKB antibodyInhibitor of nuclear factor kappa B kinase subunit alpha antibodyInhibitor of nuclear factor kappa B kinase subunit beta antibodyNFKBIKA antibodyNFKBIKB antibodyNuclear factor NF kappa B inhibitor kinase alpha antibodyNuclear factor NF kappa B inhibitor kinase beta antibodyTCF16 antibody
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Anti-IKK alpha + IKK beta antibody images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - IKK alpha + IKK beta antibody (ab62481)Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue using ab62181 (1/50 dilution)
1. Without peptide
2. With peptide
References for Anti-IKK alpha + IKK beta antibody (ab62481)
ab62481 has not yet been referenced specifically in any publications.
