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Products:Cell Biology >> Apoptosis >> Intracellular >> NFkB >> IKK
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Read our guarantee »Anti-IKK alpha antibody [Y463]
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Rabbit monoclonal [Y463] to IKK alpha
ICC, WB, IHC-P, Flow Cyt, IPmore details
Reacts with
Mouse, Rat, Human
A synthetic peptide corresponding to residues in the N terminus of human IKK alpha.
WB: Daudi cell lysate. IHC-P: Human stomach carcinoma.
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Concentration information loading...
Monoclonal
Y463
IgG
Immunology >> Innate Immunity >> TLR Signaling
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Inflammatory mediators
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> NFkB Pathway
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Cell Biology >> Apoptosis >> Intracellular >> NFkB >> IKK
Our Abpromise guarantee covers the use of ab32041 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC: 1/500.
WB: 1/10000 - 1/50000. Detects a band of approximately 88 kDa (predicted molecular weight: 85 kDa).
IHC-P: 1/50.
Flow Cyt: 1/50.
IP: 1/50.
Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. Also phosphorylates NCOA3. Phosphorylates 'Ser-10' of histone H3 at NF-kappa-B-regulated promoters during inflammatory responses triggered by cytokines.
Widely expressed.
Defects in CHUK are the cause of cocoon syndrome (COCOS) [MIM:613630]; also known as fetal encasement syndrome. COCOS is a lethal syndrome characterized by multiple fetal malformations including defective face and seemingly absent limbs, which are bound to the trunk and encased under the skin.
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
Contains 1 protein kinase domain.
Phosphorylated by MAP3K14/NIK, AKT and to a lesser extent by MEKK1, and dephosphorylated by PP2A. Autophosphorylated.
Acetylation of Thr-179 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the I-kappa-B signaling pathway.
Cytoplasm. Nucleus. Shuttles between the cytoplasm and the nucleus.
Target information above from: UniProt accessionO15111
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Western blot - IKK alpha antibody [Y463] (ab32041)
![Western blot - IKK alpha antibody [Y463] (ab32041)](/ps/datasheet/Images/32/ab32041/ab32041wb.jpg)
Anti-IKK alpha antibody [Y463] (ab32041) at 1/50000 dilution + Daudi cell lysate
Predicted band size : 85 kDa
Observed band size : 88 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Paraffin-embedded sections) - IKK alpha antibody [Y463] (ab32041)
![Immunohistochemistry (Paraffin-embedded sections) - IKK alpha antibody [Y463] (ab32041)](/ps/datasheet/Images/32/ab32041/ab32041_1a.jpg)
Ab32041, at a dilution of 1/50, staining IKK alpha in paraffin embedded human stomach carcinoma by Immunohistochemisty.
Flow Cytometry - IKK alpha antibody [Y463] (ab32041)
![Flow Cytometry - IKK alpha antibody [Y463] (ab32041)](/ps/datasheet/images/32/ab32041/IKK-alpha-Primary-antibodies-ab32041-3.jpg)
Overlay histogram showing HeLa cells stained with ab32041 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32041, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
ab32041 has not yet been referenced specifically in any publications.
Publishing research using ab32041? Please let us know so that we can cite the reference in this datasheet
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![Immunohistochemistry (Paraffin-embedded sections) - IKK alpha antibody [Y463] (ab32041)](/ps/datasheet/Images/32/ab32041/ab32041_1a.jpg)
Ab32041, at a dilution of 1/50, staining IKK alpha in paraffin embedded human stomach carcinoma by Immunohistochemisty.
![Flow Cytometry - IKK alpha antibody [Y463] (ab32041)](/ps/datasheet/images/32/ab32041/IKK-alpha-Primary-antibodies-ab32041-3.jpg)
Overlay histogram showing HeLa cells stained with ab32041 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32041, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (
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