Anti-IKK alpha antibody (ab4111)

Overview

  • Product nameAnti-IKK alpha antibodySee all IKK alpha primary antibodies ...
  • Description
    Rabbit polyclonal to IKK alpha
  • SpecificityRecognises human IKK alpha and does not cross react with IKK beta or IKK gamma.
  • Tested applicationsWB, ICC/IF more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to amino acids 716-734 of human IKK alpha (1,2), which differs from correspondingmurine sequence by four amino acids.

  • Positive control
    • Whole cell lysate from HeLa or Jurkat cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab4111 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
ICC/IF
  • Application notesICC/IF: Use at a concentration of 1 µg/ml.
    WB: At 1/1000-1/2000 dilution a 85kDa band should be detected.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionActs as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. Also phosphorylates NCOA3. Phosphorylates 'Ser-10' of histone H3 at NF-kappa-B-regulated promoters during inflammatory responses triggered by cytokines.
    • Tissue specificityWidely expressed.
    • Involvement in diseaseDefects in CHUK are the cause of cocoon syndrome (COCOS) [MIM:613630]; also known as fetal encasement syndrome. COCOS is a lethal syndrome characterized by multiple fetal malformations including defective face and seemingly absent limbs, which are bound to the trunk and encased under the skin.
    • Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Phosphorylated by MAP3K14/NIK, AKT and to a lesser extent by MEKK1, and dephosphorylated by PP2A. Autophosphorylated.
      Acetylation of Thr-179 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the I-kappa-B signaling pathway.
    • Cellular localizationCytoplasm. Nucleus. Shuttles between the cytoplasm and the nucleus.
    • Target information above from: UniProt accession O15111 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
    • Alternative names
      • chuk antibody
      • CHUK1 antibody
      • Conserved Helix Loop Helix Ubiquitous Kinase antibody
      • Conserved helix loop ubiquitous kinase antibody
      • Conserved helix-loop-helix ubiquitous kinase antibody
      • I Kappa B Kinase 1 antibody
      • I Kappa B Kinase Alpha antibody
      • I-kappa-B kinase 1 antibody
      • I-kappa-B kinase alpha antibody
      • IkappaB kinase antibody
      • IkB kinase alpha subunit antibody
      • IKBKA antibody
      • IKK 1 antibody
      • IKK A antibody
      • IKK a kinase antibody
      • IKK-A antibody
      • IKK-alpha antibody
      • IKK1 antibody
      • IKKA antibody
      • IKKA_HUMAN antibody
      • Inhibitor Of Kappa Light Polypeptide Gene Enhancer In B Cells antibody
      • Inhibitor Of Nuclear Factor Kappa B Kinase Alpha Subunit antibody
      • Inhibitor of nuclear factor kappa-B kinase subunit alpha antibody
      • NFKBIKA antibody
      • Nuclear Factor Kappa B Inhibitor Kinase Alpha antibody
      • Nuclear factor NF kappa B inhibitor kinase alpha antibody
      • Nuclear factor NF-kappa-B inhibitor kinase alpha antibody
      • Nuclear factor NFkappaB inhibitor kinase alpha antibody
      • Nuclear Factor Of Kappa Light Chain Gene Enhancer In B Cells Inhibitor antibody
      • TCF-16 antibody
      • TCF16 antibody
      • Transcription factor 16 antibody
      see all

    Anti-IKK alpha antibody images

    • Anti-IKK alpha antibody (ab4111) at 1/1000 dilution + HeLa whole cell lysate

      Observed band size : 85 kDa (why is the actual band size different from the predicted?)
    • ab4111 at 1µg/ml staining IKK alpha in Jurkat cells by ICC/IF

    • Immunofluorescence of IKK alpha in Jurkat cells using ab4111 at 10 ug/ml.

    References for Anti-IKK alpha antibody (ab4111)

    ab4111 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Sample Human Cell lysate - whole cell (HeLa cell extract)
    Loading amount 50000 cells
    Specification HeLa cell extract
    Gel Running Conditions Reduced Denaturing
    Blocking step Pierce TBS Super Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Username

    Dr. Mahesh Mathrubutham

    Verified customer

    Submitted Nov 21 2007

    All the information we have for our products is on the datasheets. As well as reviewing the applications section to determine whether ICC/IHC has been tested, I also suggest that you review the cross reactivity section. For example, this antibody -- ab...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"