Anti-IKK alpha antibody (ab4112)
- Product nameAnti-IKK alpha antibodySee all IKK alpha primary antibodies ...
- DescriptionRabbit polyclonal to IKK alpha
- SpecificityRecognises human IKK alpha and does not cross react with IKK beta or IKK gamma.
- Tested applicationsWB, ICC/IF more details
- Species reactivityReacts with: Human
Synthetic peptide corresponding to amino acids 699-715 of human IKK alpha (1,2), which differs from correspondingmurine sequence by one amino acid.
- Positive controlWhole cell lysate from HeLa cells.
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPBS with 0.02% sodium azide
- Concentration information loading...
- PurityIgG fraction
- Purification notesPurified IgG was prepared from rabbit antiserum by affinity chromatography.
- Clonality Polyclonal
Our Abpromise guarantee covers the use of ab4112 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||WB: 1/500 - 1/1000. Predicted molecular weight: 85 kDa.|
|ICC/IF||ICC/IF: Use a concentration of 10 µg/ml.|
- FunctionActs as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. Also phosphorylates NCOA3. Phosphorylates 'Ser-10' of histone H3 at NF-kappa-B-regulated promoters during inflammatory responses triggered by cytokines.
- Tissue specificityWidely expressed.
- Involvement in diseaseDefects in CHUK are the cause of cocoon syndrome (COCOS) [MIM:613630]; also known as fetal encasement syndrome. COCOS is a lethal syndrome characterized by multiple fetal malformations including defective face and seemingly absent limbs, which are bound to the trunk and encased under the skin.
- Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
Contains 1 protein kinase domain.
modificationsPhosphorylated by MAP3K14/NIK, AKT and to a lesser extent by MEKK1, and dephosphorylated by PP2A. Autophosphorylated.
Acetylation of Thr-179 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the I-kappa-B signaling pathway.
- Cellular localizationCytoplasm. Nucleus. Shuttles between the cytoplasm and the nucleus.
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Anti-IKK alpha antibody images
ab4112 at 10µg/ml staining IKK alpha in Hela cells by ICC/IF
Anti-IKK alpha antibody (ab4112) at 1/500 dilution + Hela whole cell lysate
Predicted band size : 85 kDa
Observed band size : 85 kDa
References for Anti-IKK alpha antibody (ab4112)
ab4112 has not yet been referenced specifically in any publications.