Anti-IKK alpha antibody (ab47453)

Overview

• Product nameAnti-IKK alpha antibodySee all IKK alpha primary antibodies ...
• Description
  Rabbit polyclonal to IKK alpha
• SpecificityThis antibody detects endogenous levels of total IKK alpha protein.
• Tested applicationsWB, IHC-P, ELISA more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Rat
• Immunogen

  Synthesized non phosphopeptide derived from human IKK alpha around the phosphorylation site of Threonine 23.

• Positive controlWB: Extracts from 293 cells and extracts from MDA-MB-435 cells. IHC-P: Colon carcinoma.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 50% Glycerol, PBS (without Mg²⁺ and Ca²⁺), 150mM Sodium chloride, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesThe antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Signal Transduction
  • Protein Phosphorylation
  • Ser / Thr Kinases
  • Other Kinases

  • Signal Transduction
  • Signaling Pathway
  • Nuclear Signaling
  • NFkB Pathway

  • Cardiovascular
  • Atherosclerosis
  • Vascular Inflammation
  • Inflammatory mediators

  • Immunology
  • Innate Immunity
  • TLR Signaling

  • Cell Biology
  • Apoptosis
  • Intracellular
  • NFkB
  • IKK

Applications

Target

• FunctionActs as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. Also phosphorylates NCOA3. Phosphorylates 'Ser-10' of histone H3 at NF-kappa-B-regulated promoters during inflammatory responses triggered by cytokines.
• Tissue specificityWidely expressed.
• Involvement in diseaseDefects in CHUK are the cause of cocoon syndrome (COCOS) [MIM:613630]; also known as fetal encasement syndrome. COCOS is a lethal syndrome characterized by multiple fetal malformations including defective face and seemingly absent limbs, which are bound to the trunk and encased under the skin.
• Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
  Contains 1 protein kinase domain.
• Post-translational
  modificationsPhosphorylated by MAP3K14/NIK, AKT and to a lesser extent by MEKK1, and dephosphorylated by PP2A. Autophosphorylated.
  Acetylation of Thr-179 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the I-kappa-B signaling pathway.
• Cellular localizationCytoplasm. Nucleus. Shuttles between the cytoplasm and the nucleus.

Target information above from: UniProt accession O15111 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 1147 Human
  • Entrez Gene: 1147 Human
  • Entrez Gene: 309361 Rat
  • Omim: 600664 Human
  • SwissProt: O15111 Human
  • SwissProt: O15111 Human
  • Unigene: 198998 Human

• Alternative names
  chuk antibodyCHUK1 antibodyConserved Helix Loop Helix Ubiquitous Kinase antibody

  Conserved helix loop ubiquitous kinase antibodyConserved helix-loop-helix ubiquitous kinase antibodyI Kappa B Kinase 1 antibodyI Kappa B Kinase Alpha antibodyI-kappa-B kinase 1 antibodyI-kappa-B kinase alpha antibodyIkappaB kinase antibodyIkB kinase alpha subunit antibodyIKBKA antibodyIKK 1 antibodyIKK A antibodyIKK a kinase antibodyIKK-A antibodyIKK-alpha antibodyIKK1 antibodyIKKA antibodyIKKA_HUMAN antibodyInhibitor Of Kappa Light Polypeptide Gene Enhancer In B Cells antibodyInhibitor Of Nuclear Factor Kappa B Kinase Alpha Subunit antibodyInhibitor of nuclear factor kappa-B kinase subunit alpha antibodyNFKBIKA antibodyNuclear Factor Kappa B Inhibitor Kinase Alpha antibodyNuclear factor NF kappa B inhibitor kinase alpha antibodyNuclear factor NF-kappa-B inhibitor kinase alpha antibodyNuclear factor NFkappaB inhibitor kinase alpha antibodyNuclear Factor Of Kappa Light Chain Gene Enhancer In B Cells Inhibitor antibodyTCF-16 antibodyTCF16 antibodyTranscription factor 16 antibody

  see all

Anti-IKK alpha antibody images

• 

  Western blot - IKK alpha antibody (ab47453)
  All lanes : Anti-IKK alpha antibody (ab47453) at 1 µg
  
  Lane 1 : The extracts from 293 cells treated with EGF.
  Lane 2 : The extracts from MDA-MB-435 cells treated with EGF.
  Lane 3 : The extracts from MDA-MB-435 cells, untreated.
  
  
  Predicted band size : 85 kDa
  Observed band size : 84 kDa (why is the actual band size different from the predicted?)
  
• 

  Immunohistochemistry (Paraffin-embedded sections) - IKK alpha antibody (ab47453)
  Ab47453, at dilution of 1/50, staining IKK alpha in paraffin embedded colon carcinoma tissue by Immunohistochemistry.
  Left image: Untreated.
  Right image: Treated with synthesized peptide.