Anti-IKK alpha antibody (ab63537)

Overview

• Product nameAnti-IKK alpha antibodySee all IKK alpha primary antibodies ...
• Description
  Rabbit polyclonal to IKK alpha
• SpecificityThis antibody detects endogenous levels of total IKK alpha and IKK beta protein.
• Tested applicationsWB, IHC-P, ELISA more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Synthesized non-phosphopeptide derived from human IKK alpha/IKK beta around the phosphorylation site of serine 176 (Q-G-S^P-L-C).

• Positive controlJurkat cell lysate or human skeletal muscle tissue.

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 50% Glycerol, PBS (without Mg²⁺ and Ca²⁺), 150mM Sodium chloride, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Signal Transduction
  • Protein Phosphorylation
  • Ser / Thr Kinases
  • Other Kinases

  • Signal Transduction
  • Signaling Pathway
  • Nuclear Signaling
  • NFkB Pathway

  • Cardiovascular
  • Atherosclerosis
  • Vascular Inflammation
  • Inflammatory mediators

  • Immunology
  • Innate Immunity
  • TLR Signaling

  • Cell Biology
  • Apoptosis
  • Intracellular
  • NFkB
  • IKK

Applications

Target

• FunctionActs as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. Also phosphorylates NCOA3. Phosphorylates 'Ser-10' of histone H3 at NF-kappa-B-regulated promoters during inflammatory responses triggered by cytokines.
• Tissue specificityWidely expressed.
• Involvement in diseaseDefects in CHUK are the cause of cocoon syndrome (COCOS) [MIM:613630]; also known as fetal encasement syndrome. COCOS is a lethal syndrome characterized by multiple fetal malformations including defective face and seemingly absent limbs, which are bound to the trunk and encased under the skin.
• Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
  Contains 1 protein kinase domain.
• Post-translational
  modificationsPhosphorylated by MAP3K14/NIK, AKT and to a lesser extent by MEKK1, and dephosphorylated by PP2A. Autophosphorylated.
  Acetylation of Thr-179 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the I-kappa-B signaling pathway.
• Cellular localizationCytoplasm. Nucleus. Shuttles between the cytoplasm and the nucleus.

Target information above from: UniProt accession O15111 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 1147 Human
  • Entrez Gene: 1147 Human
  • Omim: 600664 Human
  • SwissProt: O15111 Human
  • SwissProt: O15111 Human
  • Unigene: 198998 Human

• Alternative names
  chuk antibodyCHUK1 antibodyConserved Helix Loop Helix Ubiquitous Kinase antibody

  Conserved helix loop ubiquitous kinase antibodyConserved helix-loop-helix ubiquitous kinase antibodyI Kappa B Kinase 1 antibodyI Kappa B Kinase Alpha antibodyI-kappa-B kinase 1 antibodyI-kappa-B kinase alpha antibodyIkappaB kinase antibodyIkB kinase alpha subunit antibodyIKBKA antibodyIKK 1 antibodyIKK A antibodyIKK a kinase antibodyIKK-A antibodyIKK-alpha antibodyIKK1 antibodyIKKA antibodyIKKA_HUMAN antibodyInhibitor Of Kappa Light Polypeptide Gene Enhancer In B Cells antibodyInhibitor Of Nuclear Factor Kappa B Kinase Alpha Subunit antibodyInhibitor of nuclear factor kappa-B kinase subunit alpha antibodyNFKBIKA antibodyNuclear Factor Kappa B Inhibitor Kinase Alpha antibodyNuclear factor NF kappa B inhibitor kinase alpha antibodyNuclear factor NF-kappa-B inhibitor kinase alpha antibodyNuclear factor NFkappaB inhibitor kinase alpha antibodyNuclear Factor Of Kappa Light Chain Gene Enhancer In B Cells Inhibitor antibodyTCF-16 antibodyTCF16 antibodyTranscription factor 16 antibody

  see all

Anti-IKK alpha antibody images

• 

  Western blot - IKK alpha antibody (ab63537)
  All lanes : Anti-IKK alpha antibody (ab63537) at 1/500 dilution
  
  Lane 1 : Extracts from Jurkat cells (5-30ug), without immunising peptide.
  Lane 2 : Extracts from Jurkat cells (5-30ug), with immunising peptide (5-10ug).
  
  
  Predicted band size : 85 kDa
  Observed band size : 80 kDa (why is the actual band size different from the predicted?)
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - IKK alpha antibody (ab63537)
  Ab63537 (1/50-1/100) staining human IKK alpha/IKK beta in human skeletal muscle tissue by immunohistochemistry using formalin fixed, paraffin embedded tissue.
  
  The left hand panel is without immunising peptide, whereas the right hand panel is in the presence of immunising peptide.