Anti-IKK alpha (phospho S176 + S180) antibody (ab17943)
Overview
- Product nameAnti-IKK alpha (phospho S176 + S180) antibodySee all IKK alpha primary antibodies ...
- DescriptionRabbit polyclonal to IKK alpha (phospho S176 + S180)
- Tested applicationsICC/IF, WB more details
- Species reactivityReacts with: Human
Predicted to work with: Mouse - Immunogen
Synthetic phosphopeptide derived from the region of human IKKalpha that contains serine 176 and 180.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.05% Sodium Azide
Constituents: PBS (without Mg2+ and Ca2+), 1mg/ml BSA. pH 7.3 -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
-
Research Areas
Applications
Our Abpromise guarantee covers the use of ab17943 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ICC/IF | ICC/IF: Use at an assay dependent concentration. PubMed: 21887257 |
| WB | WB: Use a concentration of 0.5 - 1.5 µg/ml. Predicted molecular weight: 85 kDa. When looking at the phosphorylation of endogenous proteins, we recommend that IKKalpha first be immunoprecipitated. |
Target
- FunctionActs as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. Also phosphorylates NCOA3. Phosphorylates 'Ser-10' of histone H3 at NF-kappa-B-regulated promoters during inflammatory responses triggered by cytokines.
- Tissue specificityWidely expressed.
- Involvement in diseaseDefects in CHUK are the cause of cocoon syndrome (COCOS) [MIM:613630]; also known as fetal encasement syndrome. COCOS is a lethal syndrome characterized by multiple fetal malformations including defective face and seemingly absent limbs, which are bound to the trunk and encased under the skin.
- Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
Contains 1 protein kinase domain. - Post-translational
modificationsPhosphorylated by MAP3K14/NIK, AKT and to a lesser extent by MEKK1, and dephosphorylated by PP2A. Autophosphorylated.
Acetylation of Thr-179 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the I-kappa-B signaling pathway. - Cellular localizationCytoplasm. Nucleus. Shuttles between the cytoplasm and the nucleus.
-
Database links
- Entrez Gene: 1147 Human
- Entrez Gene: 1147 Human
- Omim: 600664 Human
- SwissProt: O15111 Human
- SwissProt: O15111 Human
- SwissProt: Q60680 Mouse
- Unigene: 198998 Human
- Unigene: 3996 Mouse
Target information above from: UniProt accession
O15111
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
.
-
Alternative names
- chuk antibodyCHUK1 antibodyConserved Helix Loop Helix Ubiquitous Kinase antibody
- Conserved helix loop ubiquitous kinase antibodyConserved helix-loop-helix ubiquitous kinase antibodyI Kappa B Kinase 1 antibodyI Kappa B Kinase Alpha antibodyI-kappa-B kinase 1 antibodyI-kappa-B kinase alpha antibodyIkappaB kinase antibodyIkB kinase alpha subunit antibodyIKBKA antibodyIKK 1 antibodyIKK A antibodyIKK a kinase antibodyIKK-A antibodyIKK-alpha antibodyIKK1 antibodyIKK1 antibodyIKKA antibodyIKKA antibodyIKKA_HUMAN antibodyInhibitor Of Kappa Light Polypeptide Gene Enhancer In B Cells antibodyInhibitor Of Nuclear Factor Kappa B Kinase Alpha Subunit antibodyInhibitor of nuclear factor kappa-B kinase subunit alpha antibodyNFKBIKA antibodyNFKBIKA antibodyNuclear Factor Kappa B Inhibitor Kinase Alpha antibodyNuclear factor NF kappa B inhibitor kinase alpha antibodyNuclear factor NF-kappa-B inhibitor kinase alpha antibodyNuclear factor NFkappaB inhibitor kinase alpha antibodyNuclear Factor Of Kappa Light Chain Gene Enhancer In B Cells Inhibitor antibodyTCF-16 antibodyTCF16 antibodyTCF16 antibodyTranscription factor 16 antibody
see all
References for Anti-IKK alpha (phospho S176 + S180) antibody (ab17943)
This product has been referenced in:
- Chung S et al. NF-?B Inducing Kinase, NIK Mediates Cigarette Smoke/TNFa-Induced Histone Acetylation and Inflammation through Differential Activation of IKKs. PLoS One 6:e23488 (2011). WB, ICC/IF ; Human . Read more (PubMed: 21887257) »
- Khan KA et al. Bcl-3-regulated transcription from major immediate-early promoter of human cytomegalovirus in monocyte-derived macrophages. J Immunol 182:7784-94 (2009). WB ; Human . Read more (PubMed: 19494302) »
