Anti-IKK alpha (phospho S176 + S180) antibody (ab17943)
- Product nameAnti-IKK alpha (phospho S176 + S180) antibodySee all IKK alpha primary antibodies ...
- DescriptionRabbit polyclonal to IKK alpha (phospho S176 + S180)
- Tested applicationsICC/IF, WB more details
- Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic phosphopeptide derived from the region of human IKKalpha that contains serine 176 and 180.
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.05% Sodium Azide
Constituents: PBS (without Mg2+ and Ca2+), 1mg/ml BSA. pH 7.3
- Concentration information loading...
- PurityImmunogen affinity purified
- Clonality Polyclonal
Our Abpromise guarantee covers the use of ab17943 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||ICC/IF: Use at an assay dependent concentration. PubMed: 21887257|
|WB||WB: Use a concentration of 0.5 - 1.5 µg/ml. Predicted molecular weight: 85 kDa. When looking at the phosphorylation of endogenous proteins, we recommend that IKKalpha first be immunoprecipitated.|
- FunctionActs as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. Also phosphorylates NCOA3. Phosphorylates 'Ser-10' of histone H3 at NF-kappa-B-regulated promoters during inflammatory responses triggered by cytokines.
- Tissue specificityWidely expressed.
- Involvement in diseaseDefects in CHUK are the cause of cocoon syndrome (COCOS) [MIM:613630]; also known as fetal encasement syndrome. COCOS is a lethal syndrome characterized by multiple fetal malformations including defective face and seemingly absent limbs, which are bound to the trunk and encased under the skin.
- Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
Contains 1 protein kinase domain.
modificationsPhosphorylated by MAP3K14/NIK, AKT and to a lesser extent by MEKK1, and dephosphorylated by PP2A. Autophosphorylated.
Acetylation of Thr-179 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the I-kappa-B signaling pathway.
- Cellular localizationCytoplasm. Nucleus. Shuttles between the cytoplasm and the nucleus.
- chuk antibodyCHUK1 antibodyConserved Helix Loop Helix Ubiquitous Kinase antibody
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References for Anti-IKK alpha (phospho S176 + S180) antibody (ab17943)
This product has been referenced in:
- Chung S et al. NF-?B Inducing Kinase, NIK Mediates Cigarette Smoke/TNFa-Induced Histone Acetylation and Inflammation through Differential Activation of IKKs. PLoS One 6:e23488 (2011). WB, ICC/IF ; Human . Read more (PubMed: 21887257) »
- Khan KA et al. Bcl-3-regulated transcription from major immediate-early promoter of human cytomegalovirus in monocyte-derived macrophages. J Immunol 182:7784-94 (2009). WB ; Human . Read more (PubMed: 19494302) »