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Product name
Anti-IKK beta antibody
See all IKK beta products (17) ...
Description
Rabbit polyclonal to IKK beta
Specificity
Recognise IKK beta and does not cross react with IKK alpha or IKK gamma.
Tested applications
IHC-P, WB, IHC-Pmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
Synthetic peptide corresponding to amino acids 662-680 of human IKK beta, which differs from correspondingmurine sequence by one amino acid.
Positive control
Whole celllysate from Jurkat cells.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
PBS with 0.02% sodium azide
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
Purified IgG was prepared by affinity chromatography.
Clonality
Polyclonal
Isotype
IgG
Research areas
Immunology >> Innate Immunity >> TLR Signaling
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Inflammatory mediators
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> NFkB Pathway
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Cell Biology >> Apoptosis >> Intracellular >> NFkB >> IKK
Applications
Show applications key
Our Abpromise guarantee covers the use of ab6146 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 2 Images
IHC-P
Application notes
IHC-P: Use at an assay dependent dilution.
western blotting - 1/500 - 1/1000
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Also phosphorylates NCOA3.
Tissue specificity
Highly expressed in heart, placenta, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis and peripheral blood.
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
Contains 1 protein kinase domain.
Post-translational
modifications
Upon cytokine stimulation, phosphorylated on Ser-177 and Ser-181 by MEKK1 and/or MAP3K14/NIK; which enhances activity. Once activated, autophosphorylates on the C-terminal serine cluster; which decreases activity and prevents prolonged activation of the inflammatory response.
Acetylation of Thr-180 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the I-kappa-B pathway.
Ubiquitinated. Monoubiquitination involves TRIM21 that leads to inhibition of Tax-induced NF-kappa-B signaling. According to PubMed:19675099, 'Ser-163' does not serve as a monoubiquitination site. According to PubMed:16267042, ubiquitination on 'Ser-163' modulates phosphorylation on C-terminal serine residues. Monoubiquitination by TRIM21 is dirupted by Yersinia yopJ.
Cellular localization
Cytoplasm. Membrane raft. Colocalized with DPP4 in membrane rafts.
Target information above from: UniProt accessionO14920
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- I kappa B kinase 2 antibody
- I kappa B kinase beta antibody
- I-kappa-B kinase 2 antibody
see all
Database links
- Entrez Gene: 3551 Human
- Entrez Gene: 16150 Mouse
- Entrez Gene: 84351 Rat
- Omim: 603258 Human
- SwissProt: O14920 Human
- SwissProt: O88351 Mouse
- SwissProt: Q9QY78 Rat
- Unigene: 597664 Human
see all
Anti-IKK beta antibody images:
Western blot - IKK beta antibody (ab6146)
Anti-IKK beta antibody (ab6146) at 1/500 dilution + Jurkat whole cell lysate
Observed band size : 87 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Paraffin-embedded sections) - IKK beta antibody (ab6146)
Immunohistochemical staining (paraffin embedded) of HeLa cells using anti-IKK beta at 10 µg/ml.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - IKK beta antibody (ab6146)
ab6146 staining IKK beta in human prostate adenocarcinoma tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using sodium citrate buffer. Samples were then blocked with 0.08% casein for 1 hour at 25°C followed by incubation with the primary antibody at a 1/1000 dilution for 1 hour at 25°C. An undiluted HRP-conjugated goat monoclonal was used as secondary antibody.
Image courtesy of Lewis MacKenzie by Abreview.
References for Anti-IKK beta antibody (ab6146)
ab6146 has not yet been referenced specifically in any publications.
Publishing research using ab6146? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"