Anti-IKK gamma antibody (ab63255)
- Product nameAnti-IKK gamma antibodySee all IKK gamma primary antibodies ...
- DescriptionRabbit polyclonal to IKK gamma
- Tested applicationsWB, ELISA, ICC/IF more details
- Species reactivityReacts with: Human
Predicted to work with: Mouse
synthesized non phosphopeptide derived from human IKK gamma around the phosphorylation site of serine 85
- Positive control
- extracts from HepG2 cells treated with Anisomycin, HeLa cells
- Storage instructionsStore at -20°C. Stable for 12 months at -20°C
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
- Concentration information loading...
- PurityImmunogen affinity purified
- Clonality Polyclonal
Our Abpromise guarantee covers the use of ab63255 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||WB: 1/500 - 1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 47 kDa).|
|ICC/IF||ICC/IF: 1/500 - 1/1000.|
- FunctionRegulatory subunit of the IKK core complex which phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Also considered to be a mediator for TAX activation of NF-kappa-B. Could be implicated in NF-kappa-B-mediated protection from cytokine toxicity (By similarity). Essential for viral activation of IRF3.
- Tissue specificityHeart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
- Involvement in diseaseDefects in IKBKG are the cause of ectodermal dysplasia anhidrotic with immunodeficiency X-linked (EDAID) [MIM:300291]; also known as hypohidrotic ectodermal dysplasia with immunodeficiency (HED-ID). Is a form of ectoderma dysplasia, a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. Characterized by absence of sweat glands, sparse scalp hair, rare conical teeth and immunological abnormalities resulting in severe infectious diseases.
Defects in IKBKG are the cause of ectodermal dysplasia anhidrotic with immunodeficiency-osteopetrosis-lymphedema (OLEDAID) [MIM:300301].
Defects in IKBKG are a cause of immunodeficiency NEMO-related without anhidrotic ectodermal dysplasia (NEMOID) [MIM:300584]; also called immunodeficiency without anhidrotic ectodermal dysplasia, isolated immunodeficiency or pure immunodeficiency. Patients manifest immunodeficiency not associated with other abnormalities, and resulting in increased infection susceptibility. Patients suffer from multiple episodes of infectious diseases.
Defects in IKBKG are the cause of susceptibility to X-linked familial atypical micobacteriosis type 1 (AMCBX1) [MIM:300636]; also known as X-linked disseminated atypical mycobacterial infection type 1 or X-linked susceptibility to mycobacterial disease type 1. AMCBX1 is the X-linked recessive form of mendelian susceptibility to mycobacterial disease (MSMD). MSMD is a congenital syndrome resulting in predisposition to clinical disease caused by weakly virulent mycobacterial species, such as bacillus Calmette-Guerin vaccines and non-tuberculous, environmental mycobacteria. Patients are also susceptible to the more virulent species Mycobacterium tuberculosis.
Defects in IKBKG are the cause of recurrent isolated invasive pneumococcal disease type 2 (IPD2) [MIM:300640]. Recurrent invasive pneumococcal disease (IPD) is defined as two episodes of IPD occurring at least 1 month apart, whether caused by the same or different serotypes or strains. Recurrent IPD occurs in at least 2% of patients in most series, making IPD the most important known risk factor for subsequent IPD.
Defects in IKBKG are the cause of incontinentia pigmenti (IP) [MIM:308300]; formerly designed familial incontinentia pigmenti type II (IP2). IP is a genodermatosis usually prenatally lethal in males. In affected females, it causes abnormalities of the skin, hair, eyes, nails, teeth, skeleton, heart, and central nervous system. The prominent skin signs occur in four classic cutaneous stages: perinatal inflammatory vesicles, verrucous patches, a distinctive pattern of hyperpigmentation and dermal scarring.
- Sequence similaritiesContains 1 C2HC-type zinc finger.
- DomainThe leucine-zipper domain and the C2HC-type zinc-finger are essential for polyubiquitin binding and for the activation of IRF3.
modificationsPhosphorylation at Ser-68 attenuates aminoterminal homodimerization.
Polyubiquitinated on Lys-285 through 'Lys-63'; the ubiquitination is mediated by NOD2 and RIPK2 and probably plays a role in signaling by facilitating interactions with ubiquitin domain-containing proteins and activates the NF-kappa-B pathway. Polyubiquitinated on Lys-399 through 'Lys-63'; the ubiquitination is mediated by BCL10, MALT1 and TRAF6 and probably plays a role in signaling by facilitating interactions with ubiquitin domain-containing proteins and activates the NF-kappa-B pathway. Monoubiquitinated on Lys-277 and Lys-309; promotes nuclear export. Linear polyubiquitinated on Lys-285; the head-to-tail polyubiquitination is mediated by the LUBAC complex. Linear polyubiquitinated on Lys-309; the head-to-tail polyubiquitination is mediated by the LUBAC complex.
Sumoylated on Lys-277 and Lys-309 by SUMO1; the modification results in phosphorylation of Ser-85 by ATM leading to a replacement of the sumoylation by mono-ubiquitination on these residues.
- Cellular localizationCytoplasm. Nucleus. Sumoylated NEMO accumulates in the nucleus in response to genotoxic stress.
- IkB kinase associated protein 1 antibody
- IkB kinase subunit gamma antibody
- Inhibitor of nuclear factor kappa B kinase subunit gamma antibody
- AMCBX1 antibody
- FIP 3 antibody
- FIP-3 antibody
- FIP3 antibody
- Fip3p antibody
- I kappa B kinase gamma antibody
- I-kappa-B kinase subunit gamma antibody
- IkB kinase gamma subunit antibody
- IkB kinase subunit gamma antibody
- IkB kinase-associated protein 1 antibody
- Ikbkg antibody
- IKK-gamma antibody
- IKKAP1 antibody
- IKKG antibody
- Incontinentia pigmenti antibody
- Inhibitor of kappa light polypeptide gene enhancer in B cells, kinase gamma antibody
- Inhibitor of nuclear factor kappa-B kinase subunit gamma antibody
- IP antibody
- IP1 antibody
- IP2 antibody
- IPD2 antibody
- NEMO antibody
- NEMO_HUMAN antibody
- NF kappa B essential modifier antibody
- NF kappa B essential modulator antibody
- NF-kappa-B essential modifier antibody
- NF-kappa-B essential modulator antibody
- ZC2HC9 antibody
Anti-IKK gamma antibody images
All lanes : Anti-IKK gamma antibody (ab63255) at 1/500 dilution
Lane 1 : extracts from HepG2 cells, treated with Anisomycin (0.5uM, 5hours), without immunising peptide
Lane 2 : extracts from HepG2 cells, treated with Anisomycin (0.5uM, 5hours), with immunising peptide
Predicted band size : 47 kDa
Observed band size : 40 kDa (why is the actual band size different from the predicted?)
Immunofluorescence analysis of IKK gamma expression in HeLa cells, using 1/500 ab63255. left image: untreated sample. Right image: sample treated with immunising peptide.
References for Anti-IKK gamma antibody (ab63255)
ab63255 has not yet been referenced specifically in any publications.