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Overview

  • Product nameIL-12 p40 Human ELISA Kit
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    < 10 pg/ml
  • Range
    12.29 pg/ml - 3000 pg/ml
  • Recovery

    100 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 127.6 120% - 134%
    Serum 99.73 91% - 108%
    Plasma 91.11 85% - 98%

  • Species reactivity
    Reacts with: Human
  • Product overview

    ab113324 IL-12 p40 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IL-12 p40 in serum, plasma, cell culture supernatants. This assay employs an antibody specific for human IL-12 p40 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-12 p40 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IL-12 p40 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-12 p40 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsELISA more details

Properties

  • Storage instructionsStore at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    20X Wash Buffer Concentrate Item B 1 x 25ml
    500X HRP-Streptavidin Concentrate Item G 1 x 200µl
    Assay Diluent B Item E 1 x 15ml
    Assay Diluent C Item L 1 x 30ml
    Detection Antibody Item F 2 vials
    IL-12 p40 Microplate (12 strips x 8 wells) coated with anti-human IL-12 p40 1 unit
    IL-12 p40 Standard Human (recombinant) 2 vials
    Stop Solution Item I 1 x 8ml
    TMB One-Step Substrate Reagent Item H 1 x 12ml
  • Research Areas
  • FunctionCytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.
    Associates with IL23A to form the IL-23 interleukin, an heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to an heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.
  • Involvement in diseaseDefects in IL12B are a cause of mendelian susceptibility to mycobacterial disease (MSMD) [MIM:209950]; also known as familial disseminated atypical mycobacterial infection. This rare condition confers predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections, with the exception of Salmonella which infects less than 50% of these individuals. The pathogenic mechanism underlying MSMD is the impairment of interferon-gamma mediated immunity, whose severity determines the clinical outcome. Some patients die of overwhelming mycobacterial disease with lepromatous-like lesions in early childhood, whereas others develop, later in life, disseminated but curable infections with tuberculoid granulomas. MSMD is a genetically heterogeneous disease with autosomal recessive, autosomal dominant or X-linked inheritance.
    Genetic variations in IL12B are a cause of susceptibility to psoriasis type 11 (PSORS11) [MIM:612599]. Psoriasis is a common, chronic inflammatory disease of the skin with multifactorial etiology. It is characterized by red, scaly plaques usually found on the scalp, elbows and knees. These lesions are caused by abnormal keratinocyte proliferation and infiltration of inflammatory cells into the dermis and epidermis.
  • Sequence similaritiesBelongs to the type I cytokine receptor family. Type 3 subfamily.
    Contains 1 fibronectin type-III domain.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
  • Post-translational
    modifications    Known to be C-mannosylated in the recombinant protein; it is not yet known for sure if the wild-type protein is also modified.
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession P29460 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        40 kD Subunit CLMFCLMF 2
        CLMF p40CLMF p40CLMF2Cytotoxic lymphocyte maturation factor 2Cytotoxic lymphocyte maturation factor 2 p40Cytotoxic lymphocyte maturation factor 40 kDa subunitCytotoxic lymphocyte maturation factor 40 kDa subunitHGNC:5970IL 12 subunit p40IL 12BIL-12 subunit p40IL-12 subunit p40IL-12BIL-12BIL12 subunit p40IL12BIL12BIL12B_HUMANIL12B_HUMANInterleukin 12 beta chainInterleukin 12 p40Interleukin 12 subunit betaInterleukin 12B Interleukin-12 subunit betaInterleukin-12 subunit betaInterleukin12BNatural killer cell stimulatory factor 2 Natural killer cell stimulatory factor 40 kD subunitNK cell stimulatory factor chain 2NK cell stimulatory factor chain 2NKSFNKSF 2NKSF2NKSF2p40Subunit P40T cell stimulating factor TcMFTSF
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab113324 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      IL-12 p40 Human ELISA Kit images

      • ELISA - IL-12 p40 Human ELISA Kit - 1 x 96 well plate (ab113324)
        ELISA - IL-12 p40 Human ELISA Kit - 1 x 96 well plate (ab113324)

      Protocols

      References for IL-12 p40 Human ELISA Kit (ab113324)

      ab113324 has not yet been referenced specifically in any publications.

      Publishing research using ab113324 ? Please let us know so that we can cite the reference in this datasheet

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      Submit an Abreview for ab113324 Submit a Question for ab113324

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      Dear vendor,

      I have a couple of technical questions:

      Ab113324; the Antibodies bind only to p40 subunit or also to p70?

      Ab64709; the Antibodies bind only to p19 subunit or also to p40?

      In other words I am trying ...

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      Thank you for enquiry.

      I am pleased to confirm the following for you:

      ab64709 IL23 Human ELISA Kit: the antibodies included in this kit have been raised against the p19 subunit.

      ab113324 IL-12 p40 Human ELISA Kit: the anti...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"