IL-17A Human ELISA Kit (ab83692)

Overview

• Product nameIL-17A Human ELISA KitSee all IL17 kits ...
• Detection methodColorimetric
• Precision

  Intra-assay

  Sample	n	Mean	SD	CV%
  A	3	49pg/ml	3.8	7.7%
  B	3	36pg/ml	2.3	6.4%
  C	3	31pg/ml	0.6	1.9%

  Inter-assay

  Sample	n	Mean	SD	CV%
  A	8	50pg/ml	7.8	14.5%
  B	8	37pg/ml	3	8.2%
  C	8	31pg/ml	2.1	6.6%

• Tests
  2 x 96 well plate
• Sample type
  Cell culture supernatant, Serum, Plasma
• Assay typeSandwich
• Sensitivity
  < 3 pg/ml
• Range
  3.12 pg/ml - 100 pg/ml
• Recovery

  105 %
  

  Sample specific recovery

  Sample type	Average %	Range
  Serum	121	113% - 130%

• Assay time
  3h 45m
• Assay durationMultiple steps standard assay
• Species reactivity
  Reacts with: Human
  
• Product overview

  INTENDED USE

  The IL-17A ELISA is to be used for the in-vitro quantitative determination of IL-17A in supernatant, buffered solutions, or serum and plasma samples. The assay will recognize natural IL-17A. This kit has been configured for research use only and is not to be used in diagnostic procedures.

  PRINCIPLE OF THE METHOD

  The IL-17A is a solid phase sandwich Enzyme Linked-Immuno- Sorbent Assay (ELISA). A monoclonal antibody specific for IL-17A has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-17A concentrations and unknowns are pipetted into these wells.

  During the first incubation, IL-17A antigen is added to wells. After washing, a biotinylated monoclonal antibody specific for IL-17A is incubated. Then the enzyme (streptavidin-peroxydase) is added. After incubation and washing to remove all unbound enzyme, a substrate solution which actes on the bound enzyme is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IL-17A present in the samples.

   

  The assay recognizes natural and recombinant human IL-17A.

  
  
  RANGE: 3.12 pg/ml - 100 pg/ml
  
  SENSITIVITY: < 3 pg/ml

  
  
  To define specificity of this ELISA, several proteins were tested for cross reactivity. There was no cross reactivity observed for any protein tested (IL-1b, IL-6, IL-23, IFNg, IL-17B, IL-17D, IL-17E, IL-17F).
  
  The minimum detectable dose of IL-17A is less than 3 pg/ml. This has been determined by adding 2 standard deviations to the mean optical density obtained when the zero standard was assayed 35 times.

• Notes

  SAFETY

  • For research use only.
  • Handling of reagents, serum or plasma specimens should be in accordance with local safety procedures , e.g.

    CDC/NIH Health manual : " Biosafety in Microbiological and Biomedical Laboratories" 1984.

  • Avoid any skin contact with H₂SO₄ and TMB. In case of contact, wash thoroughly with water.
  • Do not eat, drink, smoke or apply cosmetics where kit reagents are used.
  • Do not pipette by mouth.
  • The stop solution in this kit contains 9.8% sulphuric acid.

• Tested applicationsELISA more details
• PlatformMicroplate

Properties

• Storage instructionsStore at +4°C. Please refer to protocols.

• Components	Identifier	2 x 96 tests
  10X Standard Diluent Buffer	Black	1 x 25ml
  200X Washing Buffer	White	2 x 10ml
  Biotinylated anti IL-17A		2 x 0.4ml
  Biotinylated Antibody Diluent	Red	1 x 13ml
  Chromogen TMB		1 x 24ml
  HRP Diluent	Red	1 x 23ml
  IL17A precoated strips		12 units
  IL17A Standard: 100 pg/ml		4 vials
  Stop Reagent	Black	2 x 11ml
  Streptavidin-HRP		4 x 5µl

• Research Areas
  • Kits/ Lysates/ Other
  • Kits
  • ELISA Kits
  • ELISA Kits
  • Cytokines and cytokine receptors ELISA kits

  • Kits/ Lysates/ Other
  • Kits
  • ELISA Kits
  • ELISA Kits
  • Interleukin ELISA kits

Applications

IL-17A Human ELISA Kit images

• 

  ELISA - Human IL-17A ELISA Kit - 2 x 96 Well Plates (ab83692)
  Typical IL-17A standard curve ranging from 100 to 3.125 pg/mL

Protocols

• IL17A ELISA Kit Protocol