IL-18 R beta Human ELISA Kit (ab113325)
- Product nameIL-18 R beta Human ELISA Kit
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity< 12 pg/ml
- Range12.3 pg/ml - 3000 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 75.78 68% - 85% Serum 80.9 71% - 89% Plasma 74.98 67% - 83%
- Species reactivityReacts with: Human
- Product overview
ab113325 IL-18 R beta Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IL-18 R beta in serum, plasma, cell culture supernatants. This assay employs an antibody specific for human IL-18 R beta coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-18 R beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IL-18 R beta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-18 R beta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
- Tested applicationsELISA more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 1 x 96 tests 20X Wash Buffer Concentrate Item B 1 x 25ml 300X HRP-Streptavidin Concentrate Item G 1 x 200µl Assay Diluent B Item E 1 x 15ml Assay Diluent D Item K 1 x 15ml Detection Antibody IL-18 R beta Item F 2 vials HRP-Streptavidin Concentrate 300x concentrated HRP-conjugated streptavidin Item G 1 x 200µl IL-18 R beta Microplate (12 strips x 8 wells) coated with anti-Human IL-18 R beta Item A 1 unit IL-18 R beta Standard Human (recombinant) 2 vials Standards Item C 2 vials Stop Solution Item I 1 x 8ml TMB One-Step Substrate Reagent Item H 1 x 12ml
- Research Areas
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 TIR domain.
- Accessory protein like AcPL IL 18RAcP
- IL 1R accessory protein like IL 1R7 IL 1RAcPL IL18Rbeta Interleukin 18 receptor accessory protein Accessory protein-likeAcPLCD218 antigen-like family member BCD218bCDw218bI18RA_HUMANIL-18 receptor accessory proteinIL-18R-betaIL-18RAcPIL-18RbetaIL-1R accessory protein-likeIL-1R-7IL-1R7IL-1RAcPLIL18RAPIL18RAPInterleukin-1 receptor 7Interleukin-18 receptor accessory proteinInterleukin-18 receptor accessory protein-likeInterleukin-18 receptor beta
Our Abpromise guarantee covers the use of ab113325 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for IL-18 R beta Human ELISA Kit (ab113325)
ab113325 has not yet been referenced specifically in any publications.