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Overview

  • Product nameIL-18 R beta Human ELISA Kit
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    < 12 pg/ml
  • Range
    12.3 pg/ml - 3000 pg/ml
  • Recovery

    77 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 75.78 68% - 85%
    Serum 80.9 71% - 89%
    Plasma 74.98 67% - 83%

  • Species reactivity
    Reacts with: Human
  • Product overview

    ab113325 IL-18 R beta Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IL-18 R beta in serum, plasma, cell culture supernatants. This assay employs an antibody specific for human IL-18 R beta coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-18 R beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IL-18 R beta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-18 R beta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsELISA more details

Properties

  • Storage instructionsStore at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    20X Wash Buffer Concentrate Item B 1 x 25ml
    300X HRP-Streptavidin Concentrate Item G 1 x 200µl
    Assay Diluent B Item E 1 x 15ml
    Assay Diluent D Item K 1 x 15ml
    Detection Antibody IL-18 R beta Item F 2 vials
    HRP-Streptavidin Concentrate 300x concentrated HRP-conjugated streptavidin Item G 1 x 200µl
    IL-18 R beta Microplate (12 strips x 8 wells) coated with anti-Human IL-18 R beta Item A 1 unit
    IL-18 R beta Standard Human (recombinant) 2 vials
    Standards Item C 2 vials
    Stop Solution Item I 1 x 8ml
    TMB One-Step Substrate Reagent Item H 1 x 12ml
  • Research Areas
  • FunctionRequired for the high affinity binding of interleukin 18 (IL-18) to its receptor complex (By similarity). Together with IL18R1 mediates IL-18-dependent activation of NF-kappa-B and JNK.
  • Tissue specificityStrongly expressed in peripheral blood leukocytes, spleen, lung, and, to a lesser extent, in colon, but not in any other tissue tested. Strongly expressed in T-cells polarized with IL-12. Specifically coexpressed with IL18R1 in Th1 cells.
  • Sequence similaritiesBelongs to the interleukin-1 receptor family.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 TIR domain.
  • Cellular localizationMembrane.

    • Target information above from: UniProt accession O95256 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        Accessory protein like AcPL IL 18RAcP
        IL 1R accessory protein like IL 1R7 IL 1RAcPL IL18Rbeta Interleukin 18 receptor accessory protein Accessory protein-likeAcPLCD218 antigen-like family member BCD218bCDw218bI18RA_HUMANIL-18 receptor accessory proteinIL-18R-betaIL-18RAcPIL-18RbetaIL-1R accessory protein-likeIL-1R-7IL-1R7IL-1RAcPLIL18RAPIL18RAPInterleukin-1 receptor 7Interleukin-18 receptor accessory proteinInterleukin-18 receptor accessory protein-likeInterleukin-18 receptor beta
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab113325 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      IL-18 R beta Human ELISA Kit images

      • ELISA - IL-18 R beta Human ELISA Kit - 1 x 96 wells (ab113325)
        ELISA - IL-18 R beta Human ELISA Kit - 1 x 96 wells (ab113325)

      Protocols

      References for IL-18 R beta Human ELISA Kit (ab113325)

      ab113325 has not yet been referenced specifically in any publications.

      Publishing research using ab113325 ? Please let us know so that we can cite the reference in this datasheet

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