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IL-2 Human Elispot Kit (Reagents for 5 x 96 Well Plates) (ab46546)

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Overview

  • Product nameIL-2 Human Elispot Kit (Reagents for 5 x 96 Well Plates)See all IL2 kits ...
  • Tests
    5 x 96 well plate
  • Sample type
    Cell culture supernatant, Cell culture extracts
  • Assay typeELISPOT
  • Species reactivity
    Reacts with: Human, Monkey
  • Product overview

    The ELISPOT assay is designed to enumerate cytokine producing cells in a single cell suspension. This method has the advantage of requiring a minimum of in-vitro manipulations allowing cytokine production analysis as close as possible to in-vivo conditions in a highly specific way. This technique is designed to determine the frequency of cytokine producing cells under a given stimulation, and the follow-up of such frequency during a treatment and/or a pathological state. Elispot assay constitutes an ideal tool in the TH1 / TH2 response, vaccine development, viral infection monitoring and treatment, cancerology, infectious diseases, autoimmune diseases and transplantation.

    Principle

    The Elispot assay is based on sandwich immuno-enzyme technology. Cell secreted cytokines or soluble molecules are captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured cytokines are revealed by tracer antibodies and appropriate conjugates.

    After cell stimulation, locally produced cytokines are captured by a specific monoclonal antibody. After cell lysis, trapped cytokine molecules are revealed by a secondary biotinylated detection antibody, which is in turn recognised by streptavidin conjugated to alkaline phosphatase. PVDF-bottomed-well plates are then incubated with BCIP/NBT substrate. Colored "purple" spots indicate cytokine production by individual cells.

     

    Recognizes natural human IL2

  • Tested applicationsMeasuring secreted proteins from cells more details

Properties

  • RelevanceHuman interleukin 2 (IL2) is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. IL2 has been shown to have antitumor effects in some studies. This is probably mediated by cytotoxic effector cells.
  • Cellular localizationSecreted
    • Alternative names
        AldesleukinIL2Interleukin 2
        LymphokineT Cell Growth FactorTCGF
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab46546 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISPOT ELISPOT

      References for IL-2 Human Elispot Kit (Reagents for 5 x 96 Well Plates) (ab46546)

      ab46546 has not yet been referenced specifically in any publications.

      Publishing research using ab46546 ? Please let us know so that we can cite the reference in this datasheet

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      Submit an Abreview for ab46546 Submit a Question for ab46546

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      1) Sind die Platte im Kit ab46546 mit dabei?
      2) Kann man die PVDF-Membranen mit einem AID-Reader lesen (Fluorospotkits IL2, IFNg)?

      Vielen Dank für Ihren Anruf.

      1) Im IL2 ELISpot Kitab46546 sind nur die Reagenzien für den ELISpot enthalten, keine Platten. Den IL2 ELISpotKitMIT Platten haben wir alsab62927 erhältlich:

      http://www.abcam.com/index.html?d...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"