IL-27 Human ELISA Kit (ab83698)

Overview

• Product nameIL-27 Human ELISA KitSee all IL27 kits ...
• Precision

  Intra-assay

  Sample	n	Mean	SD	CV%
  A	9	343pg/ml	12.9	4.8%
  B	9	127pg/ml	8.1	5.1%
  C	9	427pg/ml	15.8	3.8%

  Inter-assay

  Sample	n	Mean	SD	CV%
  A	9	343pg/ml	29	8.5%
  B	9	157pg/ml	17	10.8%
  C	9	427pg/ml	38	9%

• Tests
  2 x 96 well plate
• Sample type
  Cell culture supernatant, Serum, Plasma
• Assay typeSandwich
• Sensitivity
  < 12.8 pg/ml
• Range
  31.25 pg/ml - 1000 pg/ml
• Recovery

  95 %
  

  Sample specific recovery

  Sample type	Average %	Range
  Serum	110	103% - 118%

• Assay time
  3h 45m
• Species reactivity
  Reacts with: Human
  
• Product overview

  INTENDED USE

  The IL-27 ELISA is to be used for the in-vitro quantitative determination of IL-27 in supernatants, buffered solutions, or serum and plasma samples. The assay will recognize both natural and recombinant IL-27. This kit has been configured for research use only and is not to be used in diagnostic procedures.

  PRINCIPLE OF THE METHOD

  The IL-27 Kit is a solid phase sandwich Enzyme Linked-Immuno- Sorbent Assay (ELISA). A monoclonal antibody specific for IL-27 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-27 concentrations and unknowns are pipetted into these wells. During the first incubation, IL-27 antigen is added to wells. After washing, a biotinylated monoclonal antibody specific for IL-27 is incubated. Then the enzyme ( streptavidin-peroxydase ) is added. After incubation and washing to remove all unbound enzyme, a substrate solution which actes on the bound enzyme is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IL-27 present in the samples.

   

  The assay recognizes both natural and recombinant human IL-27.

  
  
  RANGE: 31.25 pg / ml - 1000 pg / ml
  
  SENSITIVITY: < 12.8 pg / ml

  
  
  
  To define specificity of this ELISA, several proteins were tested for cross reactivity. There was no cross reactivity observed for any protein tested (IL-6, IL-6R, gp130, IL-10, IL-12, IL-12p40, IL23, ICAM-1).

• Notes

  SAFETY

  • For research use only.
  • Handling of reagents, serum or plasma specimens should be in accordance with local safety procedures , e.g.

    CDC/NIH Health manual : " Biosafety in Microbiological and Biomedical Laboratories" 1984.

  • Avoid any skin contact with H₂SO₄ and TMB. In case of contact, wash thoroughly with water.
  • Do not eat, drink, smoke or apply cosmetics where kit reagents are used.
  • Do not pipette by mouth.
  • The stop solution in this kit contains 9.8% sulphuric acid.

• Tested applicationsELISA more details

Properties

• Storage instructionsStore at +4°C. Please refer to protocols.

• Components	2 x 96 tests
  8 strips	12 units
  Biotinylated anti IL-27	2 x 0.4ml
  Biotinylated Antibody Diluent	1 x 13ml
  Chromogen TMB	1 x 24ml
  H2SO4:Stop Reagent	2 x 11ml
  HRP Diluent	1 x 23ml
  Standard Diluent: buffer	2 x 30ml
  Standard: 1000 pg/ml	4 vials
  Streptavidin-HRP	4 x 5µl
  Washing Buffer (200x Concentrated)	2 x 10ml

• Research Areas
  • Cancer
  • Tumor immunology
  • Cytokines
  • Interleukins

  • Kits/ Lysates/ Other
  • Kits
  • ELISA Kits
  • ELISA Kits
  • Cytokines and cytokine receptors ELISA kits

  • Immunology
  • Innate Immunity
  • Cytokines
  • Interleukins

Applications

IL-27 Human ELISA Kit images

• 

  ELISA - Human IL-27 ELISA Kit - 2 x 96 Well Plates (ab83698)
  Typical IL-27 standard curve ranging from 1000 to 31.25 pg/mL.

Protocols

• IL-27 ELISA Kit Protocol