Human IL-9 ELISA Kit (ab100577)
Key features and details
- Sensitivity: 0.1 ng/ml
- Range: 0.04 ng/ml - 30 ng/ml
- Sample type: Cell culture supernatant, Plasma
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
-
Product name
Human IL-9 ELISA Kit
See all IL-9 kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 0.1 ng/ml -
Range
0.04 ng/ml - 30 ng/ml -
Recovery
98 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 102.1 90% - 110% Plasma 82.4 67% - 98% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s IL-9 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-9 in plasma and cell culture supernatants. (Human IL-9 concentration is pretty low in normal plasma, it may not be detected in this assay).
This assay employs an antibody specific for Human IL-9 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-9 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-9 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-9 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
We have not been able to detect endogenous Human IL-9 in normal serum with ab100577, only in serum spiked with Human IL-9.
-
Notes
Optimization may be required with urine samples.
The standard is affinity purified.
-
Platform
Microplate
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Human IL-9 2 vials IL-9 Microplate (12 x 8 wells) 1 unit Recombinant Human IL-9 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
-
Function
Supports IL-2 independent and IL-4 independent growth of helper T-cells. -
Sequence similarities
Belongs to the IL-7/IL-9 family. -
Cellular localization
Secreted. - Information by UniProt
-
Alternative names
- Cytokine P40
- Homolog of mouse T cell and mast cell growth factor 40
- HP40
see all -
Database links
- Entrez Gene: 3578 Human
- Omim: 146931 Human
- SwissProt: P15248 Human
- Unigene: 960 Human
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab100577 has not yet been referenced specifically in any publications.