Anti-IL1 Receptor I antibody [EP409Y] (ab40774)

  Western blot - IL1 Receptor I antibody [EP409Y] (ab40774)

Anti-IL1 Receptor I antibody [EP409Y] (ab40774) at 1/2000 dilution + Jurkat cell lysate at 10 µg

Predicted band size : 80 kDa
Observed band size : 75 kDa (why is the actual band size different from the predicted?)

  Flow Cytometry - IL1 Receptor I antibody [EP409Y] (ab40774)

Overlay histogram showing Jurkat cells stained with ab40774 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab40774, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a minimal signal in Jurkat cells fixed with methanol (5 min) used under the same conditions.

Please note that Abcam do not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.