IL1 Receptor I protein (Fc Chimera) (ab83575)
Constituents: 10% Trehalose, 1% Human serum albumin
Our Abpromise guarantee covers the use of ab83575 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab83575 migrates as a broad band between 65 and 100 kDa in SDS-PAGE due to post-translation modifications, in particular glycosylation. This compares with unmodified IL1 Receptor I-Fc that has a predicted molecular mass of 63.6 kDa. ab83575 consists of 0-36% carbohydrate by weight.
ab83575 separates into a number of isoforms with a pI between 5 and 8 in 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified IL1 Receptor I-Fc that has a predicted pI of 7.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
- Antigen CD121aCD121 antigen-like family member ACD121A
- CD121a antigenD2S1473IL 1IL 1R 1IL 1R alphaIL 1RT1IL-1R-1IL-1R-alphaIL-1RT-1IL-1RT1IL1 inhibitorIL1RIL1R1IL1R1_HUMANIL1RAIL1RNIL1RT1Interleukin 1 receptor alpha type 1Interleukin 1 receptor antagonist protein (IRAP)Interleukin 1 receptor type IInterleukin receptor 1Interleukin-1 receptor alphaInterleukin-1 receptor type 1Interleukin-1 receptor type IP80
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 TIR domain.
IL1 Receptor I protein (Fc Chimera) images
Densitometry of protein isoforms visualised by 2-DE. Spot trains are normal in human proteins and highlight the presence of multiple isoforms which differ according to their level of post-translational modification. The triangle indicates the theoretical MW and pI of the protein.
1D SDS-PAGE of ab83575 before and after treatment with glycosidases to remove oligosaccharides.
Lane 1 – MW markers; Lane 2 – ab83575; Lane 3 – ab83575 treated with PNGase F to remove potential N-linked glycans; Lane 4 – ab83575 treated with a glycosidase cocktail to remove potential N- and O-linked glycans. 10 µg protein loaded per lane.Drop in MW after treatment with PNGase F indicates presence of N-linked glycans. Additional bands in lane 3 and lane 4 are glycosidase enzymes.
References for IL1 Receptor I protein (Fc Chimera) (ab83575)
ab83575 has not yet been referenced specifically in any publications.