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IL1 Receptor I protein (Fc Chimera) (ab83575)

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Overview

  • Product nameIL1 Receptor I protein (Fc Chimera)See all IL1 Receptor I proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 18-333 of Human IL1 Receptor I fused to the Fc region of human IgG1 (aa 93-330) expressed in modified human 293 cells; migrates as a broad band between 65 and 100 kDa. Swiss ID = P14778.
  • Expression hostHEK 293 cells

    • Properties

  • Purity> 95 % by SDS-PAGE
  • FormLyophilised:It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
  • Storage instructionsShipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 10% Trehalose, 1% Human serum albumin
  • Concentration information loading...
  • Sequence notesTheoretical Sequence: LEADKCKEREEKIILVSSANEIDVRPCPL NPNEHKGTITWYKDDSKTP VSTEQASRIHQHKEKLWFV PAKVEDSGHYYCVVRNSSYCLRIKISAKF VENEPNLCY NAQAIFKQKLPVAGDGGLVCPYMEFFKNENNELPKLQWY KDCKPLLLDNIHFSGVKDRLIVMNVAEKHRGNYTCHASY TYLGKQYPI TRVIEFITLEENKPTRPVIVSPANETMEV DLGSQIQLICNVTGQLSDI AYWKWNGSVIDEDDPVLGE DYYSVENPANKRRSTLITVLNISEIESRF YKHPFTCFA KNTHGIDAAYIQLIYPVTNRSSNTKVDKKVEPKSCDKTH TCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVV VDVSHEDPE VKFNWYVDGVEVHNAKTKPREEQYNSTYR VVSVLTVLHQDWLNGKEYK CRVSNKALPAPIEKTISKA KGQPREPQVYTLPPSRDELTKNQVSLTCL VKGFYPSDI AVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSR WQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab83575 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
  • Application notesSDS-PAGE: Use at an assay dependent dilution.

    ab83575 migrates as a broad band between 65 and 100 kDa in SDS-PAGE due to post-translation modifications, in particular glycosylation. This compares with unmodified IL1 Receptor I-Fc that has a predicted molecular mass of 63.6 kDa. ab83575 consists of 0-36% carbohydrate by weight.

    ab83575 separates into a number of isoforms with a pI between 5 and 8 in 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified IL1 Receptor I-Fc that has a predicted pI of 7.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        Antigen CD121aCD121 antigen-like family member ACD121A
        CD121a antigenD2S1473IL 1IL 1R 1IL 1R alphaIL 1RT1IL-1R-1IL-1R-alphaIL-1RT-1IL-1RT1IL1 inhibitorIL1RIL1R1IL1R1_HUMANIL1RAIL1RNIL1RT1Interleukin 1 receptor alpha type 1Interleukin 1 receptor antagonist protein (IRAP)Interleukin 1 receptor type IInterleukin receptor 1Interleukin-1 receptor alphaInterleukin-1 receptor type 1Interleukin-1 receptor type IP80
      see all
  • FunctionReceptor for interleukin-1 alpha (IL-1A), beta (IL-1B), and interleukin-1 receptor antagonist protein (IL-1RA). Binding to the agonist leads to the activation of NF-kappa-B. Signaling involves formation of a ternary complex containing IL1RAP, TOLLIP, MYD88, and IRAK1 or IRAK2.
  • Sequence similaritiesBelongs to the interleukin-1 receptor family.
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 TIR domain.
  • Cellular localizationMembrane.

    • Target information above from: UniProt accession P14778 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    IL1 Receptor I protein (Fc Chimera) images

    • Functional Studies - IL1 Receptor I protein (Fc Chimera) (ab83575)
      Functional Studies - IL1 Receptor I protein (Fc Chimera) (ab83575)
      Densitometry of protein isoforms visualised by 2-DE. Spot trains are normal in human proteins and highlight the presence of multiple isoforms which differ according to their level of post-translational modification. The triangle indicates the theoretical MW and pI of the protein.
    • SDS-PAGE - IL1 Receptor I protein (Fc Chimera) (ab83575)
      SDS-PAGE - IL1 Receptor I protein (Fc Chimera) (ab83575)
      1D SDS-PAGE of ab83575 before and after treatment with glycosidases to remove oligosaccharides.
      Lane 1 – MW markers; Lane 2 – ab83575; Lane 3 – ab83575 treated with PNGase F to remove potential N-linked glycans; Lane 4 – ab83575 treated with a glycosidase cocktail to remove potential N- and O-linked glycans. 10 µg protein loaded per lane.

      Drop in MW after treatment with PNGase F indicates presence of N-linked glycans. Additional bands in lane 3 and lane 4 are glycosidase enzymes.

    References for IL1 Receptor I protein (Fc Chimera) (ab83575)

    ab83575 has not yet been referenced specifically in any publications.

    Publishing research using ab83575 ? Please let us know so that we can cite the reference in this datasheet

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