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Overview

  • Product nameIL1 alpha Human ELISA KitSee all IL1 alpha kits ...
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    < 2 pg/ml
  • Range
    0.82 pg/ml - 600 pg/ml
  • Recovery

    95 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 102.25 89% - 108%
    Serum 91.89 80% - 101%
    Plasma 92.46 82% - 102%

  • Species reactivity
    Reacts with: Human
  • Product overview

    ab100560 IL1 alpha Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL1 alpha in serum, plasma and cell culture supernatants. This assay employs an antibody specific for Human IL1 alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and IL1 alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL1 alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount IL1 alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsELISA more details

Properties

  • RelevanceIL1 alpha is a member of the interleukin 1 cytokine family. It is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. IL1 alpha is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease.
    • Alternative names
        Hematopoietin 1IL 1 alphaIl 1a
        IL1 IL1 ALPHAIL1AIL1F1 Ilia Interleukin 1 alphaInterleukin 1 alpha precursor Interleukin1 alpha OTTHUMP00000203692OTTMUSP00000016430Preinterleukin 1 Pro interleukin 1Pro interleukin 1 alphaProinterleukin 1RP23-160G19.8
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab100560 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      IL1 alpha Human ELISA Kit images

      • ELISA - IL1 alpha Human ELISA Kit (ab100560)
        ELISA - IL1 alpha Human ELISA Kit (ab100560)

      Protocols

      References for IL1 alpha Human ELISA Kit (ab100560)

      ab100560 has not yet been referenced specifically in any publications.

      Publishing research using ab100560 ? Please let us know so that we can cite the reference in this datasheet

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      Submit an Abreview for ab100560 Submit a Question for ab100560

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      Votes: Highest First

      Dear Sir/Madam, how about the antibodies? You would recommend from your stock ?

      Thank you for your reply.

      We do not have any antibodies against your target of interest that we can guarantee will work in bovine samples. However based on the homology information I provided in the previous email, I think there is a good chance ...

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      I am interested in getting antibody or ELISA that show reactivity with my bovine sample (or high chance to be reactive).(better if you can provide the mouse antibody) I want to try the following antibodies, 1) MMP 3 2 MMP 13 3 IL1a 4 IL1B Thanks, Anthony

      Thank you for contacting Abcam.
      I have searched through our catalogue, and I cannot find any ELISA kits that we can guarantee will work in bovine samples, but there are a few that might work for you.
      http://www.abcam.com/IL1-alpha-Human-ELISA-Kit-...

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"