IL1 alpha Human ELISA kit (ab108864)
Overview
- Product nameIL1 alpha Human ELISA kitSee all IL1 alpha kits ...
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity= 1 pg/ml
- Range0.98 pg/ml - 250 pg/ml
- Assay time5h 00m
- Species reactivityReacts with: Human
- Product overview
Interleukin-1 alpha (IL1 alpha) is a member of the IL1 superfamily containing ILl alpha, ILl beta, and IL1Ra receptor antagonist. IL1 alpha is known as hematopoietin (IL1F1) and ILl beta as catabolin (IL1F2). IL1 alpha and ILl beta are corresponding to two different isoelectric forms, acidic pI 5 and neutral pI 7, respectively. IL1 alpha has a molecular mass of 17 kDa and consist of 159 amino acids having 26-33% homology with ILl beta. They are produced mainly by macrophages and monocytes, processed and released during apoptosis, and bind with high affinity to specific receptors on target cells. While only the mature form of ILl beta has biological activity, both the pro and mature forms of IL1 alpha are active. IL1 alpha and beta are pro-inflammatory cytokine involved in immune responses, inflammatory reactions, and hematopoiesis. IL1 alpha is an epidemal cytokine that is constitutively produced by epithelial cells and plays important role in maintenance of skin barrier function. The polymorphism of increased IL1 production in patients is associated with rheumatoid arthritis, coagulation, solid tumors, leukemias, Alzheimer’s disease, autoimmune disorders, and myocardial infarction.
ab108864 IL1 alpha Human ELISA kit is designed for detection of human IL1 alpha in plasma, serum, and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique, which measures IL1 alpha in 5 hours. A murine monoclonal antibody specific for IL1 alpha has been pre-coated onto a microplate. IL1 alpha in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for IL1 alpha, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
- Tested applicationsSandwich ELISA more details
Properties
- Storage instructionsStore at +4°C. Please refer to protocols.
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Components 1 x 96 tests Biotinylated IL1 alpha Antibody (100x): 1 x 80µl Chromogen Substrate 1 x 8ml Diluent Concentrate (10x) 1 x 30ml IL1 alpha Microplate 1 pack IL1 alpha Standard 1 unit Stop Solution 1 x 12ml Streptavidin-Peroxidase Conjugate (SP Conjugate) 1 x 80µl Wash Buffer Concentrate (20x) 1 x 30ml -
Research Areas
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Alternative names
- Hematopoietin 1IL 1 alphaIl 1a
- IL1 IL1 ALPHAIL1AIL1F1 Ilia Interleukin 1 alphaInterleukin 1 alpha precursor Interleukin1 alpha OTTHUMP00000203692OTTMUSP00000016430Preinterleukin 1 Pro interleukin 1Pro interleukin 1 alphaProinterleukin 1RP23-160G19.8
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- Entrez Gene: 3552 Human
- Omim: 147760 Human
- SwissProt: P01583 Human
Applications
Our Abpromise guarantee covers the use of ab108864 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Sandwich ELISA | sELISA |
IL1 alpha Human ELISA kit images
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Sandwich ELISA - IL1 alpha Human ELISA kit - 1 x 96 well plate (ab108864)
Protocols
References for IL1 alpha Human ELISA kit (ab108864)
ab108864 has not yet been referenced specifically in any publications.
