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Overview

  • Product nameIL1 alpha Rat ELISA KitSee all IL1 alpha kits ...
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    < 15 pg/ml
  • Range
    11 pg/ml - 8000 pg/ml
  • Recovery

    81 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 87.48 76% - 95%
    Serum 79.95 69% - 88%
    Plasma 75.73 68% - 84%

  • Species reactivity
    Reacts with: Rat
  • Product overview

    ab113350 Rat IL1 alpha ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat IL1 alpha in serum, plasma and cell culture supernatants. This assay employs an antibody specific for IL1 alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and IL1 alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat IL1 alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL1 alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applicationsELISA more details

Properties

  • RelevanceIL1 alpha is a member of the interleukin 1 cytokine family. It is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. IL1 alpha is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease.
    • Alternative names
        Hematopoietin 1IFLIFN
        IFN alphaIFN-alpha-1/13IFNA@IFNA1IFNA1_HUMANIFNA13IFNAP22IL 1 alphaIl 1a IL1 IL1 ALPHAIL1AIL1F1 Ilia Interferon AlphaInterferon alpha 1Interferon alpha 1/13Interferon alpha 1/13 precursorInterferon alpha 13Interferon alpha DInterferon alpha leukocyteInterferon alpha-1/13Interferon alpha-DInterferon leukocyteInterleukin 1 alphaInterleukin 1 alpha precursor Interleukin1 alpha LeIF DMGC138207MGC138505MGC138507OTTHUMP00000203692OTTMUSP00000016430Preinterleukin 1 Pro interleukin 1Pro interleukin 1 alphaProinterleukin 1RP23-160G19.8
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab113350 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      IL1 alpha Rat ELISA Kit images

      • ELISA - IL1 alpha Rat ELISA Kit (ab113350)
        ELISA - IL1 alpha Rat ELISA Kit (ab113350)
        Standard curve

      Protocols

      References for IL1 alpha Rat ELISA Kit (ab113350)

      ab113350 has not yet been referenced specifically in any publications.

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