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Overview

  • Product nameIL1 beta Human ELISA KitSee all IL1 beta kits ...
  • Tests
    1 x 96 well plate
  • Sample type
    Serum, Plasma, Cell culture extracts
  • Assay typeSandwich
  • Sensitivity
    = 3 pg/ml
  • Range
    3.9 pg/ml - 250 pg/ml
  • Assay time
    5h 00m
  • Species reactivity
    Reacts with: Human
  • Product overview

    IL1 beta has a wide spectrum of inflammatory, metabolic, haemopoietic, and immunological properties. IL1 beta plays a significant role in hippocampal synaptic function, and is a potential genetic marker as indicator of gastric cancer risk. High plasma level of IL1 beta is associated with rheumatoid and osteoarthritic joint disease, infectious gastroenteritis, neurodegeneration, and breast cancer. High gingival crevicular fluid levels of IL1 beta is related to type 2 diabetes.

    ab108865 IL1 beta Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the detection of Human IL1 beta in saliva, plasma, serum, and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures Human IL1 beta in less than 5 hours. A murine monoclonal antibody specific for Human IL1 beta has been pre-coated onto a 96-well microplate with removable strips. IL1 beta in standards and samples is sandwiched by the immobilized polyclonal antibody and biotinylated polyclonal antibody specific for Human IL1 beta, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The colour development is stopped and the intensity of the colour is measured.

  • Tested applicationsSandwich ELISA more details

Properties

  • FunctionProduced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
  • Sequence similaritiesBelongs to the IL-1 family.
  • DomainThe similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function.
  • Cellular localizationSecreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.

    • Target information above from: UniProt accession P01584 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        CatabolinH1Hematopoietin 1
        IFN beta inducing factor IL 1 IL 1 beta IL 1BIL-1 betaIL1 IL1 BETAIL1BIL1B_HUMANIL1F2 Interleukin 1 beta Interleukin 1 beta precursor Interleukin-1 betaLAFOAFOsteoclast activating factor Preinterleukin beta Pro interleukin 1 beta
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab108865 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      Sandwich ELISA sELISA

      IL1 beta Human ELISA Kit images

      • Sandwich ELISA - IL1 beta Human ELISA Kit (ab108865)
        Sandwich ELISA - IL1 beta Human ELISA Kit (ab108865)

      Protocols

      References for IL1 beta Human ELISA Kit (ab108865)

      ab108865 has not yet been referenced specifically in any publications.

      Publishing research using ab108865 ? Please let us know so that we can cite the reference in this datasheet

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      Submit an Abreview for ab108865 Submit a Question for ab108865

      Displaying 1 - 2 of 2 results for Abreviews and Q&A

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      Votes: Highest First

      Hola,
      Estoy buscando kits de ELISA para detección de citocinas (en particular, IL-1 beta, IL-17 e IFN gamma, pero si van bien, luego podrían ser más), pero necesitaría que detectaran citocinas tanto humanas como de rat&oac...

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      Gracias por contactarnos.


      He querido comprobar la posibilidad de presentar reactividad cruzada entre ratón y humano en los kits contra las citoquinas que mencionas.

      Tenemos varios kits contra dichas citoquinas especí...

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      I have a few questions regarding the following kits:
      Ab48478 - IL6 Human Elipair Kit (Reagents for 5 x 96 Well Plates), 5x 96 testsAb47351 - Human IL1 beta Elipair Kit (Reagents for 5 x 96 Well Plates), 1kit

      1. Are the ELISA plates supplie...

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      Thank you for contacting us.

      1) Our Elipair kits only contain the reagents needed to perform the assay, i.e. they include both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and T...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"