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IL1 beta Human Elipair Kit (Reagents for 5 x 96 Well Plates) (ab47351)

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Overview

  • Product nameIL1 beta Human Elipair Kit (Reagents for 5 x 96 Well Plates)See all IL1 beta kits ...
  • Tests
    5 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeELIPAIR
  • Sensitivity
    < 5 pg/ml
  • Range
    15.6 pg/ml - 500 pg/ml
  • Species reactivity
    Reacts with: Human
  • Product overview

    Intended Use

    This Eli-pair set is intended to measure human IL-1b in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.


    Principle of Method

    The IL-1b Eli-pair includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.

     

    Recognizes both natural and recombinant human IL1b

  • Tested applicationsELISA more details

Properties

  • RelevanceInterleukin 1 (IL1), originally known as lymphocyte activating factor (LAF), activates T cells and lymphocytes, which then proliferate and secrete interleukin 2. IL1 is primarily released from stimulated macrophages and monocytes, but also is released from several other cell types, and is thought to play a key role in inflammatory and immune responses. The two closely related agents, interleukin1 alpha (IL1 alpha) and interleukin1 beta (IL1 beta) bind to the same cell surface receptor, elicit nearly identical biological responses and share 25% homology in their amino acid sequence.
  • Cellular localizationSecreted; the lack of a specific hydrophobic segment in the precursor sequence suggests that IL1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.
    • Alternative names
        CatabolinIL1 BETAIL1B
        IL1F2interleukin 1, beta
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab47351 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA
    • Application notesELISA: Use at an assay dependent dilution.


      Not yet tested in other applications.
      Optimal dilutions/concentrations should be determined by the end user.

      • IL1 beta Human Elipair Kit (Reagents for 5 x 96 Well Plates) images

      • Eli-pair - IL1 beta Human Elipair Kit (Reagents for 5 x 96 Well Plates) (ab47351)
        Eli-pair - IL1 beta Human Elipair Kit (Reagents for 5 x 96 Well Plates) (ab47351)
        IL1 beta Standard curve ranging from 15.6 to 500 pg/ml

      References for IL1 beta Human Elipair Kit (Reagents for 5 x 96 Well Plates) (ab47351)

      ab47351 has not yet been referenced specifically in any publications.

      Publishing research using ab47351 ? Please let us know so that we can cite the reference in this datasheet

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      Submit an Abreview for ab47351 Submit a Question for ab47351

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      What species does the antibodies in this kit come from?

      Thank you for contacting Abcam.



      The antibodies of ab47351 are both monoclonals, raised in mice.



      If there is anything else I can help you with, please let me know.

      I have a few questions regarding the following kits:
      Ab48478 - IL6 Human Elipair Kit (Reagents for 5 x 96 Well Plates), 5x 96 testsAb47351 - Human IL1 beta Elipair Kit (Reagents for 5 x 96 Well Plates), 1kit

      1. Are the ELISA plates supplie...

      Read More

      Thank you for contacting us.

      1) Our Elipair kits only contain the reagents needed to perform the assay, i.e. they include both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and T...

      Read More

      Dear Tech support



      Attached is a customer question regarding kits ab48487, ab47351:


      According to the protocols we need 96-well polystyrene plates.

      Are these regular tissue culture treated plates? or specia...

      Read More

      Thank you for your enquiry.

      For ab47351 you can use 96 well ELISA plates. I can recommend to purchase ELISA plates specifically. to ensure they are of the higher quality required. I am sorry wedo not sell these from Abcam, I can recommendto do ...

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"