Overview

  • Product nameAnti-IL1 beta antibody [11E5]
    See all IL1 beta primary antibodies
  • Description
    Mouse monoclonal [11E5] to IL1 beta
  • SpecificityDoes not inhibit the biological activity of Interleukin-1.
  • Tested applicationsSuitable for: ICC/IF, ELISA, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant beta human Interleukin-1

Properties

Applications

Our Abpromise guarantee covers the use of ab8320 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration. Can be paired for ELISA with Mouse monoclonal [5C10] to IL1 beta (HRP) (ab8321).
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Target

  • FunctionPotent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells.
  • Tissue specificityExpressed in activated monocytes/macrophages (at protein level).
  • Sequence similaritiesBelongs to the IL-1 family.
  • Post-translational
    modifications
    Activation of the IL1B precursor involves a CASP1-catalyzed proteolytic cleavage. Processing and secretion are temporarily associated.
  • Cellular localizationCytoplasm, cytosol. Lysosome. Secreted, exosome. Cytoplasmic vesicle, autophagosome. Secreted. The precursor is cytosolic. In response to inflammasome-activating signals, such as ATP for NLRP3 inflammasome or bacterial flagellin for NLRC4 inflammasome, cleaved and secreted. IL1B lacks any known signal sequence and the pathway(s) of its secretion is(are) not yet fully understood (PubMed:24201029). On the basis of experimental results, several unconventional secretion mechanisms have been proposed. 1. Secretion via secretory lysosomes: a fraction of CASP1 and IL1B precursor may be incorporated, by a yet undefined mechanism, into secretory lysosomes that undergo Ca(2+)-dependent exocytosis with release of mature IL1B (PubMed:15192144). 2. Secretory autophagy: IL1B-containing autophagosomes may fuse with endosomes or multivesicular bodies (MVBs) and then merge with the plasma membrane releasing soluble IL1B or IL1B-containing exosomes (PubMed:24201029). However, autophagy impacts IL1B production at several levels and its role in secretion is still controversial. 3. Secretion via exosomes: ATP-activation of P2RX7 leads to the formation of MVBs containing exosomes with entrapped IL1B, CASP1 and other inflammasome components. These MVBs undergo exocytosis with the release of exosomes. The release of soluble IL1B occurs after the lysis of exosome membranes (By similarity). 4. Secretion by microvesicle shedding: activation of the ATP receptor P2RX7 may induce an immediate shedding of membrane-derived microvesicles containing IL1B and possibly inflammasome components. The cytokine is then released in the extracellular compartment after microvesicle lysis (PubMed:11728343). 5. Release by translocation through permeabilized plasma membrane. This may occur in cells undergoing pyroptosis due to sustained activation of the inflammasome (By similarity). These mechanisms may not be not mutually exclusive.
  • Information by UniProt
  • Database links
  • Alternative names
    • Catabolin antibody
    • H1 antibody
    • IL 1 antibody
    • IL 1 beta antibody
    • IL-1 beta antibody
    • IL1 BETA antibody
    • IL1B antibody
    • IL1B_HUMAN antibody
    • IL1F2 antibody
    • Interleukin 1 beta antibody
    • Interleukin-1 beta antibody
    • OAF antibody
    • OTTHUMP00000162031 antibody
    • Preinterleukin 1 beta antibody
    • Pro interleukin 1 beta antibody
    see all

Anti-IL1 beta antibody [11E5] images

  • ab8320 staining IL1 beta in lung inflammatory cells from Rhesus monkey by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with Tween 20 and blocked with 10% serum for 2 hours at 21°C. Samples were incubated with primary antibody (1/25 in PBS + 10% serum + 1% Tween 20) for 18 hour at 4°C. An diluted Alexa Fluor®594-conjugated + 1.4nm Goat anti-mouse IgG polyclonal was used as the secondary antibody.

    See Abreview

  • Immunohistochemical analysis of frozen Human skin tissue taken from patients with localized cutaneous leishmaniasis (A, C), diffuse cutaneous leishmaniasis (E). Normal tissue (G) was used as a negative control.

    IL1 beta was stained using ab8320 at 1/100 dilution for 1 hour at room temperature. ab94705 Mouse and Rabbit Specific HRP/AEC Detection IHC Kit was used to detect staining. Hematoxylin was used as a counterstain.

References for Anti-IL1 beta antibody [11E5] (ab8320)

This product has been referenced in:
  • Cassol E  et al. CD14(+) macrophages that accumulate in the colon of African AIDS patients express pro-inflammatory cytokines and are responsive to lipopolysaccharide. BMC Infect Dis 15:430 (2015). Read more (PubMed: 26475133) »
  • Pan CY  et al. Epinecidin-1 has immunomodulatory effects, facilitating its therapeutic use in a mouse model of Pseudomonas aeruginosa sepsis. Antimicrob Agents Chemother 58:4264-74 (2014). Read more (PubMed: 24820078) »

See all 6 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Rhesus monkey Cell (Lung - inflammatory cells)
Specification Lung - inflammatory cells
Fixative Formaldehyde
Permeabilization Yes - Tween-20
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Mar 29 2013

I am glad that the Rhesus tissue worked well! Unfortunately the homology for ab8320 with sheep is only 60%, to low for any reactivity. Another IL1-beta antibody, ab45692 does have 88% homology but has only been tested for Western Blot. Please let me kn...

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Thank you very much for contacting us. To our knowledge, ab8320 has not been tested in rhesus monkey. The immunogen for this product is full length human IL1 beta, which has more than 90% homology with the Rhesus sequence. Based on this, I would predic...

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Gracias por contactarnos de nuevo.

Contáctanos tantas veces como sea necesario, no es ningún problema, estaré encantada de poder ayudar.

Para detectar IL1 beta por Sándwich ELISA, ab8320 es una buena opci...

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Thank you for your enquiry and your interest in our products. Recommended positive control for this antibody can be activated human lymphocytes. We hope this will help.

Thank you for your email. I would suggest starting with a range of dilutions, 1:50, 1:100, 1:200, and then optimize from there based on your results. If you have any more questions, please contact us again.

Yes, we this antibody is routinely used in this application, although we have not tested it ourselves.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"