Anti-IL1 beta antibody [11E5] (ab8320)

Overview

• Product nameAnti-IL1 beta antibody [11E5]See all IL1 beta primary antibodies ...
• Description
  Mouse monoclonal [11E5] to IL1 beta
• SpecificityDoes not inhibit the biological activity of Interleukin-1.
• Tested applicationsICC/IF, ELISA, IHC-P, IHC-Fr, Sandwich ELISA more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Recombinant beta human Interleukin-1

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPBS with 0.1% sodium azide, pH 7.4
• Concentration information loading...
• PurityProtein A purified
• Clonality Monoclonal
• Clone number11E5
• Myelomax63-Ag8.653
• IsotypeIgG2b
• Research Areas
  • Metabolism
  • Types of disease
  • Obesity

  • Cardiovascular
  • Atherosclerosis
  • Vascular Inflammation
  • Inflammatory mediators

  • Immunology
  • Innate Immunity
  • Cytokines
  • Interleukins

Applications

Target

• FunctionProduced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
• Sequence similaritiesBelongs to the IL-1 family.
• DomainThe similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function.
• Cellular localizationSecreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.

Target information above from: UniProt accession P01584 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 3553 Human
  • Omim: 147720 Human
  • SwissProt: P01584 Human
  • Unigene: 126256 Human

• Alternative names
  Catabolin antibodyH1 antibodyHematopoietin 1 antibody

  IFN beta inducing factor antibodyIL 1 antibodyIL 1 beta antibodyIL 1B antibodyIL-1 beta antibodyIL1 BETA antibodyIL1B antibodyIL1B_HUMAN antibodyIL1F2 antibodyInterleukin 1 beta antibodyInterleukin 1 beta precursor antibodyInterleukin-1 beta antibodyLAF antibodyOAF antibodyOsteoclast activating factor antibodyOTTHUMP00000162031 antibodyPreinterleukin 1 beta antibodyPreinterleukin beta antibodyPro interleukin 1 beta antibody

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Anti-IL1 beta antibody [11E5] images

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  Immunocytochemistry/ Immunofluorescence - Anti-IL1 beta antibody [11E5] (ab8320)This image is courtesy of an anonymous Abreview

  ab8320 staining IL1 beta in lung inflammatory cells from Rhesus monkey by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with Tween 20 and blocked with 10% serum for 2 hours at 21°C. Samples were incubated with primary antibody (1/25 in PBS + 10% serum + 1% Tween 20) for 18 hour at 4°C. An diluted Alexa Fluor®594-conjugated + 1.4nm Goat anti-mouse IgG polyclonal was used as the secondary antibody.

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  Immunohistochemistry (Frozen sections) - Anti-IL1 beta antibody [11E5] (ab8320)Image from Fernández-Figueroa EA et al., PLoS Negl Trop Dis. 2012;6(5):e1533. Epub 2012 May 22. Fig 3.; doi:10.1371/journal.pntd.0001533; May 22, 2012, PLoS Negl Trop Dis 6(5): e1533.
  Immunohistochemical analysis of frozen Human skin tissue taken from patients with localized cutaneous leishmaniasis (A, C), diffuse cutaneous leishmaniasis (E). Normal tissue (G) was used as a negative control.
  
  IL1 beta was stained using ab8320 at 1/100 dilution for 1 hour at room temperature. ab94705 Mouse and Rabbit Specific HRP/AEC Detection IHC Kit was used to detect staining. Hematoxylin was used as a counterstain.