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Recombinant beta human Interleukin-1
Our Abpromise guarantee covers the use of ab8320 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent dilution. Can be paired for ELISA with Mouse monoclonal [5C10] to IL1 beta (HRP) (ab8321).|
|IHC-P||Use at an assay dependent dilution.|
|IHC-Fr||Use at an assay dependent dilution.|
|Sandwich ELISA||Use at an assay dependent dilution. Can be paired for Sandwich ELISA with Mouse monoclonal [5C10] to IL1 beta (HRP) (ab8321). Can be used as Capture antibody with ab8321. For 2 site enzyme immunoassay, we recommend to use a mixture of this antibody and ab8319 for coating, in conjunction with ab8321 for labeling.|
ab8320 staining IL1 beta in lung inflammatory cells from Rhesus monkey by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with Tween 20 and blocked with 10% serum for 2 hours at 21°C. Samples were incubated with primary antibody (1/25 in PBS + 10% serum + 1% Tween 20) for 18 hour at 4°C. An diluted Alexa Fluor®594-conjugated + 1.4nm Goat anti-mouse IgG polyclonal was used as the secondary antibody.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"