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Product name
Anti-IL1 beta antibody [8516.311]
See all IL1 beta products (28) ...
Description
Mouse monoclonal [8516.311] to IL1 beta
Specificity
By ELISA and immunoblotting, the antibody shows < 5% cross-reactivity with rhIL1 alpha, rmIL1 beta. In addition the antibody shows no cross-reactivity with rmIl1 alpha, rhIL1 ralpha, rmIL1 ralpha, and rrIL1 ralpha. The antibody will not neutralize the biological activity of rhIL1 alpha, rmIL1 alpha or rmIL1 beta.
Tested applications
ELISA, WB, Neutralisingmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant human IL1 beta (rhIL1 beta), expressed in E. coli.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Purification notes
The antibody is purified by Protein A affinity chromatography.
Clonality
Monoclonal
Clone number
8516.311
Isotype
IgG1
Research areas
Metabolism >> Types of disease >> Obesity
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Inflammatory mediators
Immunology >> Innate Immunity >> Cytokines >> Interleukins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab10749 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 31 kDa.
Neut: Use at an assay dependent dilution.
Target
Function
Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
Sequence similarities
Belongs to the IL-1 family.
Domain
The similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function.
Cellular localization
Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.
Target information above from: UniProt accessionP01584
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Catabolin antibody
- H1 antibody
- Hematopoietin 1 antibody
see all
Database links
- Entrez Gene: 3553 Human
- Omim: 147720 Human
- SwissProt: P01584 Human
- Unigene: 126256 Human
References for Anti-IL1 beta antibody [8516.311] (ab10749)
This product has been referenced in:
- Lucchesi W et al. Differential gene regulation by Epstein-Barr virus type 1 and type 2 EBNA2. J Virol 82:7456-66 (2008). WB, ELISA; Human.Read more (PubMed: 18480445) »
- Pioli PA et al. Lipopolysaccharide-induced IL-1 beta production by human uterine macrophages up-regulates uterine epithelial cell expression of human beta-defensin 2. J Immunol 176:6647-55 (2006). ELISA; Human.Read more (PubMed: 16709823) »
See all 2 publications for this product
Publishing research using ab10749? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"