Anti-IL1 beta antibody [AS10] (FITC) (ab16168)

Overview

• Product nameAnti-IL1 beta antibody [AS10] (FITC)See all IL1 beta primary antibodies ...
• Description
  Mouse monoclonal [AS10] to IL1 beta (FITC)
• ConjugationFITC
• SpecificityDoes not recognise human IL1 alpha or a panel of other human cytokines
• Tested applicationsFlow Cyt more details
• Species reactivity
  Reacts with: Human
  

  Does not react with

  Mouse
• Immunogen

  Unfortunately, this information is considered to be commercially sensitive

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.08% Sodium Azide
  Constituents: PBS
• Concentration information loading...
• PurityProtein A purified
• Purification notesAntibody purity > 95 % by SDS PAGE
• Clonality Monoclonal
• Clone numberAS10
• IsotypeIgG1
• Research Areas
  • Metabolism
  • Types of disease
  • Obesity

  • Cardiovascular
  • Atherosclerosis
  • Vascular Inflammation
  • Inflammatory mediators

  • Immunology
  • Innate Immunity
  • Cytokines
  • Interleukins

Applications

Target

• FunctionProduced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
• Sequence similaritiesBelongs to the IL-1 family.
• DomainThe similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function.
• Cellular localizationSecreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.

Target information above from: UniProt accession P01584 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 3553 Human
  • Omim: 147720 Human
  • SwissProt: P01584 Human
  • Unigene: 126256 Human

• Alternative names
  Catabolin antibodyH1 antibodyHematopoietin 1 antibody

  IFN beta inducing factor antibodyIL 1 antibodyIL 1 beta antibodyIL 1B antibodyIL-1 beta antibodyIL1 BETA antibodyIL1B antibodyIL1B_HUMAN antibodyIL1F2 antibodyInterleukin 1 beta antibodyInterleukin 1 beta precursor antibodyInterleukin-1 beta antibodyLAF antibodyOAF antibodyOsteoclast activating factor antibodyOTTHUMP00000162031 antibodyPreinterleukin 1 beta antibodyPreinterleukin beta antibodyPro interleukin 1 beta antibody

  see all

Anti-IL1 beta antibody [AS10] (FITC) images

• 

  Flow Cytometry-Anti-IL1 beta antibody [AS10] (FITC)(ab16168)
  Overlay histogram showing peripheral blood lymphocytes cells stained with ab16168 (red line). The cells were fixed with 4% paraformaldehydel (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16168, 0.5µg/1x10⁶ cells) for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 (0.5µg/1x10⁶ cells ). Acquisition of >5,000 events was performed.