Overview

  • Product nameAnti-IL1 beta antibodySee all IL1 beta primary antibodies ...
  • Description
    Rabbit polyclonal to IL1 beta
  • SpecificityPrimarily directed against mature 17kDa human IL-1beta. In ELISA formats and other immunoreactive assays, the antibody will recognize 10% of the non-denatured (native) 31kDa IL-1beta precursor containing samples, but will detect all of the 17kDa mature molecule. Also detects primate IL-1beta in the same formats using similar dilutions. The antiserum does not recognize human IL-1alpha.
  • Tested applicationsWB, IP, ELISA, IHC-P, RIA, IHC-Fr, Functional Studies, Flow Cyt, Neutralising, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Non Human Primates
  • Immunogen

    Recombinant 153 aa human IL-1beta produced in E.coli. MW of recombinant IL-1beta 17kDa, with the N-terminal amino acid at position alanine 117. This is the cleavage site generated by the IL-1beta converting enzyme (ICE, capase-1).

  • General notesImmunohistochemistry 1/100 to 1/200 (paraffin or cryofixation can be used; 1/100 for staining intracellular IL-1beta). ELISA 1/200 to 1/1000 (this antibody is best used as the second antibody with a monoclonal as a capture antibody). Radio immunoassay 1/8000 Neutralization of IL-1beta activity in bioassays 1/100 ( >4 hours incubation, normal rabbit IgG as negative control) FACS analysis - caution, F(c) domain of rabbit IgG may interact with cells non-specifically.

Properties

Applications

Our Abpromise guarantee covers the use of ab2105 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.

1/1000 - 1/2000 (antigen - supernatants or lysates of 2 x 106 endotoxin-stimulated human PBMC. Denatured 31kDa precursor IL-1beta will be recognized, but often migrates as a 35 kDa band).

IP Use at an assay dependent concentration. 1/400 - 1/800 (pre-clearing with a non-specific rabbit IgG is helpful to reduce background).
ELISA 1/500 - 1/2000.
IHC-P 1/100 - 1/200.
RIA 1/8000.
IHC-Fr 1/100 - 1/200.
Functional Studies Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. F(c) domain of rabbit IgG may interact with cells non-specifically!
Neutralising 1/100. Neutralization of IL-1beta activity in bioassays 1/100 ( >4 hours incubation, normal rabbit IgG as negative control). ab2105 does not neutralize the biological activity of murine, rat or rabbit IL1 beta.
ICC/IF Use at an assay dependent concentration.

Target

  • FunctionProduced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
  • Sequence similaritiesBelongs to the IL-1 family.
  • DomainThe similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function.
  • Cellular localizationSecreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.
  • Information by UniProt
  • Database links
  • Alternative names
    • Catabolin antibody
    • H1 antibody
    • Hematopoietin 1 antibody
    • IFN beta inducing factor antibody
    • IL 1 antibody
    • IL 1 beta antibody
    • IL 1B antibody
    • IL-1 beta antibody
    • IL1 BETA antibody
    • IL1B antibody
    • IL1B_HUMAN antibody
    • IL1F2 antibody
    • Interleukin 1 beta antibody
    • Interleukin 1 beta precursor antibody
    • Interleukin-1 beta antibody
    • LAF antibody
    • OAF antibody
    • Osteoclast activating factor antibody
    • OTTHUMP00000162031 antibody
    • Preinterleukin 1 beta antibody
    • Preinterleukin beta antibody
    • Pro interleukin 1 beta antibody
    see all

Anti-IL1 beta antibody images

  • ab2105 staining IL1 beta in Human monocytes by Flow Cytometry. Cells were fixed with paraformaldehyde and permeabilized with 0.1% saponin in PBS. The sample was incubated with the primary antibody (1/500 in 0.1% saponin + 1% FCS in PBS) for 45 minutes at 4°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG (1/100) was used as the secondary antibody.

    1 - unstimulated monocytes. 2 - monocytes stimulated with LPS.

    See Abreview

  • All lanes : Anti-IL1 beta antibody (ab2105) at 1/1000 dilution

    Lane 1 : Human peripheral blood mononuclear cell lysate - PBMC's unstimulated
    Lane 2 : Human peripheral blood mononuclear cell lysate - monocytes unstimulated
    Lane 3 : Human peripheral blood mononuclear cell lysate - monocytes stimulated with LPS
    Lane 4 : Human peripheral blood mononuclear cell lysate - positive control (rh IL-1 beta protein)

    Lysates/proteins at 25 µg per lane.

    Secondary
    HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/2000 dilution

    Performed under reducing conditions.

    Observed band size : 35 kDa (why is the actual band size different from the predicted?)


    Exposure time : 10 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

  • Lane 1 :
    Lanes 2 - 5 : Anti-IL1 beta antibody (ab2105) at 1/1000 dilution

    Lane 1 : Ladder (top to bottom: p35, p25, p20, p17, p11)
    Lane 2 : Human THP-1 leukemic monocyte cell lysate, no LPS at 20 µg
    Lane 3 : Human THP-1 leukemic monocyte supernatant, no LPS at 20 µg
    Lane 4 : Human THP-1 leukemic monocyte cell lysate, LPS stimulation at 20 µg
    Lane 5 : Human THP-1 leukemic monocyte supernatant, LPS stimulation at 20 µg

    Secondary
    Lanes 2 - 5 : IRDye® 800CW-conjugated Donkey anti-rabbit IgG monoclonal at 1/5000 dilution


    Performed under reducing conditions.

    Observed band size : 17,30 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds

    This image is courtesy of an anonymous Abreview

    Both mature and pro-IL-1beta bands, p17 and p31 were detected by ab2105 in supernatant from THP-1 cells stimulated with LPS. Only pro-IL-1beta band (p31) was detected in supernatant from THP-1 cells not stimulated with LPS.

    See Abreview

  • ab2105 at 1/20 staining human liver tissue sections by IHC-P. The cells were paraformaldehyde fixed and a heat mediated antigen retrieval step was performed. The tissue was incubated with the tissue overnight at room temperature. A biotinylated donkey anti-rabbit IgG was used as the secondary antibody and this was detected using streptavidin HRP.

    See Abreview

  • ab2105 staining IL1 beta in human keratinocytes by Immunocytochemistry/ Immunofluorescence. Cells were fixed in methanol, permeabilized in 0,1% Saponin / PBS and then blocked using 4% BSA for 30 minutes at 25°C. Samples were then incubated with ab2105 at a 1/100 dilution for 2 hours at 4°C. An Alexa-fluor 488 conjugated goat anti-rabbit polyclonal was used as the secondary antibody at a 1/100 dilution.

    See Abreview

  • ab2105 staining IL1 beta in rat alveolar macrophage tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

    Tissue was fixed with paraformaldehyde and blocked with blocking agent for 30 minutes at room temperature; antigen retrieval was by heat mediation with a citrate buffer. Samples were incubated with primary antibody (1/100 in diluent) for 2 hours. An HRP-conjugated rabbit monoclonal IgG (1/500) was used as the secondary antibody.

    See Abreview

References for Anti-IL1 beta antibody (ab2105)

This product has been referenced in:
  • Doitsh G  et al. Cell death by pyroptosis drives CD4 T-cell depletion in HIV-1 infection. Nature 505:509-14 (2014). Read more (PubMed: 24356306) »
  • Zhen J  et al. AIM2 mediates inflammation-associated renal damage in hepatitis B virus-associated glomerulonephritis by regulating caspase-1, IL-1ß, and IL-18. Mediators Inflamm 2014:190860 (2014). WB, IHC-P ; Human . Read more (PubMed: 24701032) »

See all 13 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Sample Human Tissue sections (lung)
Specification lung
Permeabilization Yes - 0,1% Saponin / PBS
Fixative Methanol
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Submitted Oct 14 2013

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6,0
Sample Human Tissue sections (skin)
Specification skin
Permeabilization Yes - 0,1% Saponin/PBS
Fixative Paraformaldehyde
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Submitted Oct 11 2013

Application ELISA
Sample Human Cell (supernatens from macrophages)
Specification supernatens from macrophages
Type Sandwich (Capture)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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Submitted Oct 08 2013

Application Flow Cytometry
Fixation Paraformaldehyde
Permeabilization Yes - 0,1% Saponin / PBS
Sample Human Cell (human monocytes)
Specification human monocytes
Gating Strategy no gate
Preparation Cell harvesting/tissue preparation method: isolated by gradient centrifugation and followed by magnetic isolation
Sample buffer: 0,1% Saponin /1% FCS/ PBS
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Submitted Oct 08 2013

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Non-Denaturing (Native) (5-16% gel)
Sample Human Cell lysate - whole cell (peripheral blood mononuclear cells)
Specification peripheral blood mononuclear cells
Treatment see below
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Oct 07 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (12%)
Sample Human Cell lysate - whole cell (THP-1 human leukemic monocyte)
Specification THP-1 human leukemic monocyte
Treatment 100 nM PMA for 24 hrs, 100 ng/ml LPS for 4 hrs
Blocking step Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 22°C
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Submitted Aug 12 2013


We have 3 IL-1B antibodies that have been tested in IHC-P and WB with human samples and that as well state to recognize the active/mature form:

ab2105, ab34837, ab106015
http://www.abcam.com/il1-beta-antibody-ab2105.html
http://ww...

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Vielen Dank für Ihre Anfrage die Herr Hryca uns in den technischen Dienst weitergeleitet hat.

Ich habe geschaut, und wir haben viele möglichen positiven Kontrollen für Sie. Können Sie mir bitte noch sagen, welche Art von p...

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According to our records, ab2105 was proving difficult to use in WB and we were in contact in order to help resolve the issue.

Looking at our correspondence, it appears that we are awaiting more details in order to help us better understand ...

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Thank you for contacting us.

To our knowledge, customised protocols are not required for this product. Any general WB protcol will work.

The dilution 1/100-1/200 are recommended for guidelines only. If you observe high background try ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"