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Recombinant IL1 beta (Human) produced in E.coli. The MW of the recombinant 153 amino acid IL1 beta was 17 kDa with the N terminal amino acid at position alanine 117. This cleavage site is generated by the IL1 beta converting enzyme (ICE, Capase 1).
For Western blotting, heating the sample in SDS with or without reducing agents will facilitate denaturing of the 31,000 MW IL1 beta precursor molecule, which often migrates as a 35,000 kDa band. This is due to the unfolding of the denatured precursor IL1 beta exposing epitopes not exposed in the natural state.
Our Abpromise guarantee covers the use of ab34837 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||1/500 - 1/5000.|
|WB||Use 2µg for 106 cells. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).|
|IP||1/400 - 1/800.|
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||1/200 - 1/400.|
|Neutralising||1/200. Incubate with antiserum for at least 4 hours before testing. A control of similarly diluted normal rabbit IgG is recommended.|
ab34837 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"