Anti-IL1 beta antibody (ab34837)

Overview

• Product nameAnti-IL1 beta antibodySee all IL1 beta primary antibodies ...
• Description
  Rabbit polyclonal to IL1 beta
• SpecificityThis antibody primarily recognizes the 17kDa mature form of IL1 beta. It also recognizes 10% of the precursor, 31kDa form of IL1 beta in cell lysates. The antibody does not react with IL1 alpha.
• Tested applicationsELISA, RIA, WB, IP, IHC-P, IHC-Fr, Neutralising more details
• Species reactivity
  Reacts with: Dog, Human
  Predicted to work with: Non Human Primates

  Does not react with

  Mouse, Rat, Rabbit
• Immunogen

  Recombinant IL1 beta (Human) produced in E.coli. The MW of the recombinant 153 amino acid IL1 beta was 17 kDa with the N terminal amino acid at position alanine 117. This cleavage site is generated by the IL1 beta converting enzyme (ICE, Capase 1).

• Positive controlSupernatants or cell lysates from cultures of LPS stimulated human peripheral blood mononuclear cells. (PBMCs were stimulated for 24 hours with 1% human serum and 10ng/ml E.coli derived LPS.)

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.01% Sodium Azide
  Constituents: Whole serum
• PurityWhole antiserum
• Purification notesThis antibody is sterile filtered and has been heated at 56°C for 30 minutes. Endotoxin content: <10pg/µl by LAL method.
• Primary antibody notes For Western blotting, heating the sample in SDS with or without reducing agents will facilitate denaturing of the 31,000 MW IL1 beta precursor molecule, which often migrates as a 35,000 kDa band. This is due to the unfolding of the denatured precursor IL1 beta exposing epitopes not exposed in the natural state.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Types of disease
  • Obesity

  • Cardiovascular
  • Atherosclerosis
  • Vascular Inflammation
  • Inflammatory mediators

  • Immunology
  • Innate Immunity
  • Cytokines
  • Interleukins

Applications

Target

• FunctionProduced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
• Sequence similaritiesBelongs to the IL-1 family.
• DomainThe similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function.
• Cellular localizationSecreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.

Target information above from: UniProt accession P01584 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 403974 Dog
  • Entrez Gene: 3553 Human
  • Omim: 147720 Human
  • SwissProt: Q28292 Dog
  • SwissProt: P01584 Human
  • Unigene: 33592 Dog
  • Unigene: 126256 Human

• Alternative names
  Catabolin antibodyH1 antibodyHematopoietin 1 antibody

  IFN beta inducing factor antibodyIL 1 antibodyIL 1 beta antibodyIL 1B antibodyIL-1 beta antibodyIL1 BETA antibodyIL1B antibodyIL1B_HUMAN antibodyIL1F2 antibodyInterleukin 1 beta antibodyInterleukin 1 beta precursor antibodyInterleukin-1 beta antibodyLAF antibodyOAF antibodyOsteoclast activating factor antibodyOTTHUMP00000162031 antibodyPreinterleukin 1 beta antibodyPreinterleukin beta antibodyPro interleukin 1 beta antibody

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