Overview

  • Product nameAnti-IL1 beta antibodySee all IL1 beta primary antibodies ...
  • Description
    Rabbit polyclonal to IL1 beta
  • SpecificityThis antibody primarily recognizes the 17kDa mature form of IL1 beta. It also recognizes 10% of the precursor, 31kDa form of IL1 beta in cell lysates. The antibody does not react with IL1 alpha.
  • Tested applicationsELISA, RIA, WB, IP, IHC-P, IHC-Fr, Neutralising more details
  • Species reactivity
    Reacts with: Dog, Human
    Predicted to work with: Non Human Primates

    Does not react with

    Mouse, Rat, Rabbit
  • Immunogen

    Recombinant IL1 beta (Human) produced in E.coli. The MW of the recombinant 153 amino acid IL1 beta was 17 kDa with the N terminal amino acid at position alanine 117. This cleavage site is generated by the IL1 beta converting enzyme (ICE, Capase 1).

  • Positive control
    • Supernatants or cell lysates from cultures of LPS stimulated human peripheral blood mononuclear cells. (PBMCs were stimulated for 24 hours with 1% human serum and 10ng/ml E.coli derived LPS.)
  • General notes


    For Western blotting, heating the sample in SDS with or without reducing agents will facilitate denaturing of the 31,000 MW IL1 beta precursor molecule, which often migrates as a 35,000 kDa band. This is due to the unfolding of the denatured precursor IL1 beta exposing epitopes not exposed in the natural state.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferConstituents: Whole serum, 0.42% Potassium phosphate, 0.88% Sodium chloride
  • PurityWhole antiserum
  • Purification notesThis antibody is sterile filtered and has been heated at 56°C for 30 minutes. Endotoxin content: <10pg/µl by LAL method.
  • Primary antibody notes For Western blotting, heating the sample in SDS with or without reducing agents will facilitate denaturing of the 31,000 MW IL1 beta precursor molecule, which often migrates as a 35,000 kDa band. This is due to the unfolding of the denatured precursor IL1 beta exposing epitopes not exposed in the natural state.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab34837 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ELISA 1/500 - 1/5000.
RIA 1/5000.
WB Use 2µg for 106 cells. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).
IP 1/400 - 1/800.
IHC-P Use at an assay dependent dilution.
IHC-Fr 1/200 - 1/400.
Neutralising 1/200. Incubate with antiserum for at least 4 hours before testing. A control of similarly diluted normal rabbit IgG is recommended.

Target

  • FunctionProduced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
  • Sequence similaritiesBelongs to the IL-1 family.
  • DomainThe similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function.
  • Cellular localizationSecreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.
  • Target information above from: UniProt accession P01584 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • Catabolin antibody
    • H1 antibody
    • Hematopoietin 1 antibody
    • IFN beta inducing factor antibody
    • IL 1 antibody
    • IL 1 beta antibody
    • IL 1B antibody
    • IL-1 beta antibody
    • IL1 BETA antibody
    • IL1B antibody
    • IL1B_HUMAN antibody
    • IL1F2 antibody
    • Interleukin 1 beta antibody
    • Interleukin 1 beta precursor antibody
    • Interleukin-1 beta antibody
    • LAF antibody
    • OAF antibody
    • Osteoclast activating factor antibody
    • OTTHUMP00000162031 antibody
    • Preinterleukin 1 beta antibody
    • Preinterleukin beta antibody
    • Pro interleukin 1 beta antibody
    see all

References for Anti-IL1 beta antibody (ab34837)

ab34837 has not yet been referenced specifically in any publications.

Product Wall


We have 3 IL-1B antibodies that have been tested in IHC-P and WB with human samples and that as well state to recognize the active/mature form:

ab2105, ab34837, ab106015
http://www.abcam.com/il1-beta-antibody-ab2105.html
http://ww...

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Thank you very much for taking the time to send further information and images.

Having reviewed the protocol details, and after all the optimisation attempts I the antibody should have given the expected staining.

My suggestions, in...

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Thank you very much for your interest our products. To our knowledge, ab34837 has not been tested in horse. However by participating in our AbTrial program you can now use our products in an untested application or species without financial risk.
...

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Thank you for contacting us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

Often it is possible to make suggestions that help resolve problems. We will happily offer technical support and in the...

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Thank you for your inquiry.

Since the antibody is produce against whole-protein immunogen, it is expected to cross react with similar proteins, but it is not tested specifically against the forms you described.

Regarding the blockin...

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Thank you for your telephone enquiry earlier this afternoon.

I can confirm that both these antibodies can be kept in the fridge at 4oC overnight until your colleagues return tomorrow.They will then need to store as directed:

For a...

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Merci pour ces précisions. Comme convenu j'ai procédé à l'envoi d'une unité de ab82558. Le numéro de commande de remplacement gratuit de ce produit est *********. Vous recevrez prochainement un mail de confirmation comprenant les détails d'expédition. ...

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Suite à votre appel, j'ai regardé les différents anti-IL1 que nous avons dans notre catalogue. Après élimination des anticorps testés avec les protéines recombinantes et non avec des lysats, j'ai retenu les références suivantes : - ab82558 : bande ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"