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Overview

  • Product nameAnti-IL1 beta antibodySee all IL1 beta primary antibodies ...
  • Description
    Rabbit polyclonal to IL1 beta
  • SpecificityAb53175 detects endogenous levels of fragment of activated IL1 beta resulting from cleavage adjacent to Asp116. Ab53175 also detects the uncleaved form in WB.
  • Tested applicationsWB, IHC-P, ELISA more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide derived from human IL1 beta.

  • Positive controlHuman lung carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab53175 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: Use at an assay dependent dilution.
IHC-P IHC-P: Use at an assay dependent dilution.
ELISA ELISA: 1/10000.

Target

  • FunctionProduced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
  • Sequence similaritiesBelongs to the IL-1 family.
  • DomainThe similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function.
  • Cellular localizationSecreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.

    • Target information above from: UniProt accession P01584 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        Catabolin antibodyH1 antibodyHematopoietin 1 antibody
        IFN beta inducing factor antibodyIL 1 antibodyIL 1 beta antibodyIL 1B antibodyIL-1 beta antibodyIL1 BETA antibodyIL1B antibodyIL1B_HUMAN antibodyIL1F2 antibodyInterleukin 1 beta antibodyInterleukin 1 beta precursor antibodyInterleukin-1 beta antibodyLAF antibodyOAF antibodyOsteoclast activating factor antibodyOTTHUMP00000162031 antibodyPreinterleukin 1 beta antibodyPreinterleukin beta antibodyPro interleukin 1 beta antibody
      see all

    Anti-IL1 beta antibody images

    • Immunohistochemistry (Paraffin-embedded sections) - IL1 beta antibody - Cleaved Asp116 (ab53175)
      Immunohistochemistry (Paraffin-embedded sections) - IL1 beta antibody - Cleaved Asp116 (ab53175)
      ab53175 at 1/50 dilution staining IL1 beta in human lung carcinoma by Immunohistochemsitry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.

    References for Anti-IL1 beta antibody (ab53175)

    ab53175 has not yet been referenced specifically in any publications.

    Publishing research using ab53175 ? Please let us know so that we can cite the reference in this datasheet

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    Thank you so much for the replacement. It worked nicely at a 1/100 dilution for the IHC. I am going to place an order for another one now, as we will try it for IF.

    That is good news, thanks for the update. If you have any trouble with the IF stain, please contact us. Good luck!

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"