Overview

  • Product nameAnti-IL1 beta antibodySee all IL1 beta primary antibodies ...
  • Description
    Rabbit polyclonal to IL1 beta
  • Tested applicationsWB, IHC-P more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chimpanzee, Macaque Monkey
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human IL1 beta.

  • Positive control
    • This antibody gave a positive signal in Jurkat and MOLT4 whole cell lysates. IHC-P: human spleen FFPE tissue sections.

Properties

Applications

Our Abpromise guarantee covers the use of ab82558 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 30 kDa).
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionProduced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
  • Sequence similaritiesBelongs to the IL-1 family.
  • DomainThe similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function.
  • Cellular localizationSecreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.
  • Target information above from: UniProt accession P01584 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • Catabolin antibody
    • H1 antibody
    • IL 1 antibody
    • IL 1 beta antibody
    • IL-1 beta antibody
    • IL1 BETA antibody
    • IL1B antibody
    • IL1B_HUMAN antibody
    • IL1F2 antibody
    • Interleukin 1 beta antibody
    • Interleukin-1 beta antibody
    • OAF antibody
    • OTTHUMP00000162031 antibody
    • Preinterleukin 1 beta antibody
    • Pro interleukin 1 beta antibody
    see all

Anti-IL1 beta antibody images

  • All lanes : Anti-IL1 beta antibody (ab82558) at 1 µg/ml

    Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 2 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 30 kDa
    Observed band size : 17 kDa (why is the actual band size different from the predicted?)


    Exposure time : 20 minutes
  • IHC image of IL1 beta staining in human spleen formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab82558, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-IL1 beta antibody (ab82558)

ab82558 has not yet been referenced specifically in any publications.

Product Wall

Merci pour ces précisions. Comme convenu j'ai procédé à l'envoi d'une unité de ab82558. Le numéro de commande de remplacement gratuit de ce produit est *********. Vous recevrez prochainement un mail de confirmation comprenant les détails d'expédition. ...

Read More

Suite à votre appel, j'ai regardé les différents anti-IL1 que nous avons dans notre catalogue. Après élimination des anticorps testés avec les protéines recombinantes et non avec des lysats, j'ai retenu les références suivantes : - ab82558 : bande ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"