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Product name
Anti-IL1 beta antibody
See all IL1 beta products (28) ...
Description
Rabbit polyclonal to IL1 beta
Tested applications
WB, ELISA, Neutralising, ICC, IHC-Fr, ICC/IFmore details
Cross reactivity
Reacts with
Mouse
Immunogen
Highly pure (>98%) recombinant mIL-1b (mouse Interleukin-1-beta).
Properties
Form
Lyophilised:
Reconstitute with 200µl of sterile water.
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
PBS, pH 7.4, no preservative, sterile filtered
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
unknown
Light chain type
unknown
Research areas
Metabolism >> Types of disease >> Obesity
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Inflammatory mediators
Immunology >> Innate Immunity >> Cytokines >> Interleukins
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Immunocytochemistry/ Immunofluorescence - IL1 beta antibody (ab9722)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab9722 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution. To detect mIL-1b by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-1b is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
ELISA: Use at an assay dependent dilution. To detect mIL-1b by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mIL-1b.
Neut: Use at an assay dependent dilution. To yield one-half maximal inhibition [ND50] of the biological activity of mIL-1b (50 pg/ml), a concentration of 100 - 150 ng/ml of this antibody is required.
ICC: 1/100.
IHC-Fr: Use at an assay dependent dilution. PubMed: 18420712Acetone fixed.
ICC/IF: Use at an assay dependent concentration.
Target
Function
Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
Sequence similarities
Belongs to the IL-1 family.
Domain
The similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function.
Cellular localization
Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.
Target information above from: UniProt accessionP01584
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Catabolin antibody
- H1 antibody
- Hematopoietin 1 antibody
see all
Database links
- Entrez Gene: 16176 Mouse
- SwissProt: P10749 Mouse
- Unigene: 222830 Mouse
Anti-IL1 beta antibody images:
Immunocytochemistry/ Immunofluorescence - IL1 beta antibody (ab9722)
ab9722 staining IL1 beta in murine bone marrow-derived macrophages by Immunocytochemistry/ Immunofluorescence. Cells are immortalised murine bone marrow-derived macrophages stably transfected with GFP-LC3 (green) to visualise autophagosomes. The cells were fixed in paraformaldehyde, permeabilised in 0.01% Triton X-100 and then blocked using 5% serum for 1 hour at 20°C. Samples were then incubated with primary antibody at 1/200 for 1 hour at 20°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 568 (red) used undiluted.
Image courtesy of James Harris by Abreview.
References for Anti-IL1 beta antibody (ab9722)
This product has been referenced in:
- Cortes-Canteli M et al. CCAAT/enhancer binding protein {beta} deficiency provides cerebral protection following excitotoxic injury. J Cell Sci 121:1224-34 (2008). WB, ICC/IF; Mouse.Read more (PubMed: 18388310) »
- Dixon LJ et al. Caspase-1-mediated regulation of fibrogenesis in diet-induced steatohepatitis. Lab Invest 92:713-23 (2012). WB; Mouse.Read more (PubMed: 22411067) »
See all 12 publications for this product
Publishing research using ab9722? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"