Overview

  • Product nameAnti-IL1 beta antibodySee all IL1 beta primary antibodies ...
  • Description
    Rabbit polyclonal to IL1 beta
  • Tested applicationsWB, ELISA, Neutralising, IHC-P, ICC, IHC-Fr, IHC-FoFr, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Highly pure (>98%) recombinant mIL-1b (mouse Interleukin-1-beta).

  • Positive control
    • FFPE mouse kidney tissue sections

Properties

Applications

Our Abpromise guarantee covers the use of ab9722 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. To detect mIL-1b by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-1b is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
ELISA Use at an assay dependent dilution. To detect mIL-1b by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mIL-1b.
Neutralising Use at an assay dependent dilution. To yield one-half maximal inhibition [ND50] of the biological activity of mIL-1b (50 pg/ml), a concentration of 100 - 150 ng/ml of this antibody is required.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/100.
IHC-Fr Use at an assay dependent dilution. PubMed: 18420712Acetone fixed.
IHC-FoFr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

  • FunctionProduced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
  • Sequence similaritiesBelongs to the IL-1 family.
  • DomainThe similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function.
  • Cellular localizationSecreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.
  • Information by UniProt
  • Database links
  • Alternative names
    • Catabolin antibody
    • H1 antibody
    • Hematopoietin 1 antibody
    • IFN beta inducing factor antibody
    • IL 1 antibody
    • IL 1 beta antibody
    • IL 1B antibody
    • IL-1 beta antibody
    • IL1 BETA antibody
    • IL1B antibody
    • IL1B_HUMAN antibody
    • IL1F2 antibody
    • Interleukin 1 beta antibody
    • Interleukin 1 beta precursor antibody
    • Interleukin-1 beta antibody
    • LAF antibody
    • OAF antibody
    • Osteoclast activating factor antibody
    • OTTHUMP00000162031 antibody
    • Preinterleukin 1 beta antibody
    • Preinterleukin beta antibody
    • Pro interleukin 1 beta antibody
    see all

Anti-IL1 beta antibody images

  • ab9722 staining IL1 beta in murine bone marrow-derived macrophages by Immunocytochemistry/ Immunofluorescence. Cells are immortalised murine bone marrow-derived macrophages stably transfected with GFP-LC3 (green) to visualise autophagosomes. The cells were fixed in paraformaldehyde, permeabilised in 0.01% Triton X-100 and then blocked using 5% serum for 1 hour at 20°C. Samples were then incubated with primary antibody at 1/200 for 1 hour at 20°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 568 (red) used undiluted.

    See Abreview

  • ab9722 staining IL1 beta in rat brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections).

    Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/100 in diluent) for 10 hours at 25°C. An AlexaFluor®555-conjugated donkey anti-goat IgG polyclonal (1/300) was used as the secondary antibody.

    See Abreview

  • ab9722 staining IL1 beta (green) in murine macrophage cells by Immunocytochemistry/ Immunofluorescence.

    Cells were fixed with formaldehyde, permeabilized with 0.1% Triton X-100 + 3% BSA and blocked with 3% BSA for 3 hours at 25°C. Samples were incubated with primary antibody (1/100 in 1% BSA in PBS) for 1 hour at 25°C. A FITC-conjugated goat anti-rabbit polyclonal IgG (1/100) was used as the secondary antibody. Nuclei were stained with DAPI (blue).
  • IHC image of IL1 beta staining in mouse kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9722, 1µg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • Immunohistochemical analysis of PFA perfusion fixed frozen mouse brain, staining IL1 beta (green) with ab9722 at 0.5 µg/ml. A peroxidase conjugated secondary antibody was used and staining was detected using a fluorescein Tyramide Signal Amplification (TSA™) reagent.

References for Anti-IL1 beta antibody (ab9722)

This product has been referenced in:
  • Sui Y  et al. IKKß links vascular inflammation to obesity and atherosclerosis. J Exp Med 211:869-86 (2014). Mouse . Read more (PubMed: 24799533) »
  • Phillips KL  et al. Interleukin-1 receptor antagonist deficient mice provide insights into pathogenesis of human intervertebral disc degeneration. Ann Rheum Dis N/A:N/A (2013). IHC ; Mouse . Read more (PubMed: 23396662) »

See all 18 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (RAW264.7)
Specification RAW264.7
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton + 2% BSA in PBS
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 24°C
Username

Dr. Mahesh Shivananjappa

Verified customer

Submitted Apr 12 2013

Thank you for contacting us. There is no MSDS for ab9722, as it contains only PBS and no hazardous chemicals.

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Macrophages cell line)
Specification Macrophages cell line
Fixative Formaldehyde
Permeabilization Yes - 0.1% triton-X100, in 3%BSA in PBS
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Dr. ananoumous ananoumous

Verified customer

Submitted Sep 11 2012

Thank you for your enquiry.

As far as we are aware, ab9722 IL1 beta antibodyhas been tested and guaranteed in mouse but nottested in rat. the other ab9787IL1 beta antibody is tested and guaranteed for rat but not tested in mouse.

...

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Application Immunocytochemistry
Sample Mouse Cultured Cells (RAW267.4)
Specification RAW267.4
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Abcam user community

Verified customer

Submitted Jul 06 2012

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Brain)
Specification Brain
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jul 04 2012

Thank you for your reply, and I apologize that I didn't properly caption the image that I sent.

The Western blot was run on murine recombinant mature IL1 beta, not on cell lysates. The lab does not test this antibody with cell lysates.
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Thank you for your call last week and for your patience while I have been in touch with the lab.

The lab tests this antibody with recombinant murine IL1 beta, and a strong band around 17 kDa is seen in addition to a band around 34 kDa that is...

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Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (RAW267.4 Macrophage)
Specification RAW267.4 Macrophage
Fixative Paraformaldehyde
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Abcam user community

Verified customer

Submitted Jun 07 2012

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"