Overview

  • Product nameAnti-IL1 beta antibodySee all IL1 beta primary antibodies ...
  • Description
    Rabbit polyclonal to IL1 beta
  • SpecificityThis antibody is used in our Rat IL-1beta EDK and was tested against the following rat growth factors at 50ng/ml to determine if there was any cross reactivity: GM-CSF, IL1alpha, IL-2, IL-4, IL-10, SCF, & TNFalpha. No significant cross reactivity was detected. The antibody has not been tested against endogenous IL1beta, only recombinant protein and we cannot guarantee it will detect endogenous protein.
  • Tested applicationsWB, ELISA, Neutralising, IHC-Fr, IHC-P, IHC-FoFr more details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Highly pure (>98%) recombinant rIL-1b (rat Interleukin-1-beta).

  • Positive control
    • recombinant rat IL1 beta (lower limit of detection: 1.95 ng/lane)

Properties

Applications

Our Abpromise guarantee covers the use of ab9787 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB Use a concentration of 0.1 - 0.2 µg/ml.
ELISA Use a concentration of 0.5 µg/ml.
Neutralising Use at an assay dependent dilution. To yield one-half maximal inhibition [ND50] of the biological activity of rIL-1b (0.30 ng/ml), a concentration of 0.003 - 0.005 µg/ml of this antibody is required.
IHC-Fr Use at an assay dependent dilution. PubMed: 20187933
IHC-P Use at an assay dependent concentration. PubMed: 22315633
IHC-FoFr Use at an assay dependent dilution. PubMed: 20649973

Target

  • FunctionProduced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
  • Sequence similaritiesBelongs to the IL-1 family.
  • DomainThe similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function.
  • Cellular localizationSecreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.
  • Target information above from: UniProt accession P01584 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • Catabolin antibody
    • H1 antibody
    • Hematopoietin 1 antibody
    • IFN beta inducing factor antibody
    • IL 1 antibody
    • IL 1 beta antibody
    • IL 1B antibody
    • IL-1 beta antibody
    • IL1 BETA antibody
    • IL1B antibody
    • IL1B_HUMAN antibody
    • IL1F2 antibody
    • Interleukin 1 beta antibody
    • Interleukin 1 beta precursor antibody
    • Interleukin-1 beta antibody
    • LAF antibody
    • OAF antibody
    • Osteoclast activating factor antibody
    • OTTHUMP00000162031 antibody
    • Preinterleukin 1 beta antibody
    • Preinterleukin beta antibody
    • Pro interleukin 1 beta antibody
    see all

Anti-IL1 beta antibody images

  • All lanes : Anti-IL1 beta antibody (ab9787) at 0.2 µg/ml

    Lane 1 : MultiMark MultiColor Standard (Invitrogen)
    Lane 2 : 250 ng/lane
    Lane 3 : 125 ng/lane
    Lane 4 : 62.5 ng/lane
    Lane 5 : 31.25 ng/lane
    Lane 6 : 15.6 ng/lane
    Lane 7 : 7.8 ng/lane
    Lane 8 : 3.9 ng/lane
    Lane 9 : 1.95 ng/lane


    Observed band size : 17.3 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of rat endometrial tissue, staining IL1 beta with ab9787.

    Tissue was taken from pregnant rats with implantation failure. Antigen retrieval was by heat mediation in a citrate buffer (pH 6) followed by blocking with 1% BSA for 30 minutes. Sections were incubated with primary antibody (1/50) overnight at 4°C. An HRP-goat anti-rabbit IgG was used as the secondary antibody (1/200). Staining was detected using DAB.
  • Immunohistochemical analysis of rat brain tissue, staining IL1 beta with ab9787.

    Tissue was taken from rats with middle cerebral artery occlusion either untreated (left) or treated with specific MEK1/2 inhibitor U0126. Samples were fixed for 10 minutes in ice-cold acetone and then rehydrated in PBS containing 0.3% Triton X-100 for 15 minutes. The tissues were then permeabilized and blocked for 1 hour in blocking solution containing PBS, 0.3% Triton X-100, 1% BSA, and 5% normal donkey serum. Samples were incubated overnight at 4°C with primary antibody (1/400) and a Cy2-conjugated donkey anti-rabbit IgG was used as the secondary antibody.

References for Anti-IL1 beta antibody (ab9787)

This product has been referenced in:
  • Oliveira-Pelegrin GR  et al. Cellular bioenergetics changes in magnocellular neurons may affect copeptin expression in the late phase of sepsis. J Neuroimmunol 267:28-34 (2014). Read more (PubMed: 24360909) »
  • Gui J  et al. Effects of acupuncture on Th1, th2 cytokines in rats of implantation failure. Evid Based Complement Alternat Med 2012:893023 (2012). IHC-P ; Rat . Read more (PubMed: 22315633) »

See all 7 Publications for this product

Product Wall

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Spinal cord)
Specification Spinal cord
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization Yes - 0.3% Triton-X100 in blocking buffer
Blocking step normal goat serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
Username

Abcam user community

Verified customer

Submitted Dec 03 2012

Application Western blot
Sample Rat Cell lysate - whole cell (Macrophages and dentritic cells)
Loading amount 15 µg
Specification Macrophages and dentritic cells
Gel Running Conditions Reduced Denaturing (4-20%)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

Submitted Oct 05 2012


I am very pleased to hear you would like to accept our offer and test ab9787 in WB (endogenous protein). This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for IHC-P and inc...

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Thank you for your message.

My colleague Padamjeet is out of office today. I can confirm that the expiration date: 17-12-2012.

Apologiesfor this mistake. If you need any further assistance in the future, please do not hesitate to co...

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Expiration date: 17-08-2012

I am very pleased to hear you would like to accept our offer and test ab9787 in [Mouse]. This code will give you: 1 free [PRIMARY ANTIBODY/PROTEIN] OR VALUE OFF OF ORDER before the expiration date. To redeem th...

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Thank you for your enquiry.

As far as we are aware, ab9722 IL1 beta antibodyhas been tested and guaranteed in mouse but nottested in rat. the other ab9787IL1 beta antibody is tested and guaranteed for rat but not tested in mouse.

...

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Thank you for your reply.


Unfortunately I am not sure what the identity of the extra bands might be. The protein you are using is not 100% pure so possibly other contaminants or strong multimers? It is reassuring that the antibody is reco...

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Thank you for your reply.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number XXX.

To check the s...

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Thank you for your patience as I have investigated this issue further with the laboratory.


Based on your results and those obtained in our own laboratory it is not clear why this antibody is detecting a 23kDa band in your samples. I am ha...

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Thank you for contacting Abcam regarding ab9787.


I have reviewed the data you sent and I was hoping you would be able to send me some more information regarding the experimental setup and WB protocol.


In particularwhat are y...

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