IL10 + IL4 Human Fluorospot Kit (ab46663)
- Product nameIL10 + IL4 Human Fluorospot KitSee all IL10 + IL4 kits ...
- Tests10 x 96 well plate
- Sample typeCell culture supernatant, Cell culture extracts
- Assay typeELISPOT
- Species reactivityReacts with: Human
- Product overview
The ELISPOT assay is designed to enumerate cytokine producing cells in a single cell suspension. This method has the advantage of requiring a minimum of in-vitro manipulations allowing cytokine production analysis as close as possible to in-vivo conditions in a highly specific way. This technique is designed to determine the frequency of cytokine producing cells under a given stimulation, and the follow-up of such frequency during a treatment and/or a pathological state. Elispot assay constitutes an ideal tool in the TH1 / TH2 response, vaccine development, viral infection monitoring and treatment, cancerology, infectious diseases, autoimmune diseases and transplantation.
The Elispot assay is based on sandwich immuno-enzyme technology. Cell secreted cytokines or soluble molecules are captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured cytokines are revealed by tracer antibodies and appropriate conjugates.
After cell stimulation, locally produced cytokines are captured by IL-10 and IL-4 specific monoclonal antibodies. After cell lysis, trapped cytokine molecules are revealed by a secondary anti- IL-10 FITC conjugated antibody and a biotinylated anti-IL-4 antibody. Those are in turn recognised by anti-FITC green fluorescent dye and streptavidin-phycoerythrin conjugates. PVDF-bottomed-well plates are then read under a UV light beam. Green fluorescent spots indicate IL-10 production while IL-4 is revealed by red spots. Yellow spots will indicate dual cytokine producing cells.
- Tested applicationsFluorospot more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 10 x 96 tests 96 PVDF-bottomed-well plates 1 unit Anti-FITC antibody green fluorescence conjugate. 2 vials Biotinylated detection antibody for IL-4 (resuspend in 0.55mL). 2 x 0.55ml Bovine Serum albumin. 2 x 1g Capture antibody for IL-10 2 x 0.5ml Capture antibody for IL-4 2 x 0.5ml FITC conjugated detection antibody for IL-10 (resuspend in 0.55mL). 2 x 0.55ml Fluorenscence buffer 2 x 2.5ml Streptavidin-phycoerythrin conjugate. 2 vials
- Research Areas
- B cell stimulatory factor 1BinetrakinBSF1
- CSIFInterleukin 10Interleukin 4PitrakinraTGIF
Our Abpromise guarantee covers the use of ab46663 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for IL10 + IL4 Human Fluorospot Kit (ab46663)
ab46663 has not yet been referenced specifically in any publications.